JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE A

Accepted manuscript available online (unedited version)

A two-photon polymerization based microfluidic biochip incorporating a herringbone microchannel and deterministic lateral displacement design for efficient capture of circulating tumor cells

Author(s): Xinyi LIANG^1, ², Changwei QIN^1, ², Kaiqiang LI³, An REN^1, ², Jiarui HU^1, ², Weikang LV^1, ², Lunan KE^1, ², Zhen WANG³, Mengfei YU⁴, Xiuxiu JIANG⁵, Huayong YANG^1, ², Xiaobin XU⁶, Liang MA^1, ²
Affiliation(s): ¹State Key Laboratory of Fluid Power and Mechatronic Systems, Zhejiang University, Hangzhou 310058, China ²School of Mechanical Engineering, Zhejiang University, Hangzhou 310058, China ³Center for Laboratory Medicine, Allergy center, Department of Transfusion medicine, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou 310014, China The Affiliated Stomatologic Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China ⁵Zhejiang Provincial Clinical Research Center for Gynecology, Zhejiang Key Laboratory of Maternal and Infant Health, Department of Family Planning, Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China ⁶School of Materials Science of Engineering, Tongji University, Shanghai 201804, China
Corresponding email(s): Liang MA: liangma@zju.edu.cn Xiaobin XU: xiaobinxu@tongji.edu.cn
Key Words: Circulating tumor cell; Cell sorting; Microfluidic chip

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Xinyi LIANG^1,², Changwei QIN^1,², Kaiqiang LI³, An REN^1,², Jiarui HU^1,², Weikang LV^1,², Lunan KE^1,², Zhen WANG³, Mengfei YU⁴, Xiuxiu JIANG⁵, Huayong YANG^1,², Xiaobin XU⁶, Liang MA^1,². A two-photon polymerization based microfluidic biochip incorporating a herringbone microchannel and deterministic lateral displacement design for efficient capture of circulating tumor cells[J]. Journal of Zhejiang University Science A,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.A2500379

@article{title="A two-photon polymerization based microfluidic biochip incorporating a herringbone microchannel and deterministic lateral displacement design for efficient capture of circulating tumor cells",
author="Xinyi LIANG^1,², Changwei QIN^1,², Kaiqiang LI³, An REN^1,², Jiarui HU^1,², Weikang LV^1,², Lunan KE^1,², Zhen WANG³, Mengfei YU⁴, Xiuxiu JIANG⁵, Huayong YANG^1,², Xiaobin XU⁶, Liang MA^1,²",
journal="Journal of Zhejiang University Science A",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.A2500379"
}

%0 Journal Article
%T A two-photon polymerization based microfluidic biochip incorporating a herringbone microchannel and deterministic lateral displacement design for efficient capture of circulating tumor cells
%A Xinyi LIANG^1
%A²
%A Changwei QIN^1
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%A Kaiqiang LI³
%A An REN^1
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%A Jiarui HU^1
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%A Weikang LV^1
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%A Lunan KE^1
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%A Zhen WANG³
%A Mengfei YU⁴
%A Xiuxiu JIANG⁵
%A Huayong YANG^1
%A²
%A Xiaobin XU⁶
%A Liang MA^1
%A²
%J Journal of Zhejiang University SCIENCE A
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%@ 1673-565X
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/jzus.A2500379"

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A1 - Lunan KE^{1
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A1 - Zhen WANG³
A1 - Mengfei YU⁴
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doi="https://doi.org/10.1631/jzus.A2500379"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Circulating tumor cells (CTCs) are cells that become detached from a primary tumor and enter the vascular or lymphatic system. These cells contain nearly the entire genetic information of the primary tumor. Enrichment and detection of CTCs play a crucial role in prognostications and risk assessments of tumor metastasis and recurrence, evaluation of efficacy and potential medications for precision tumor therapy, and detection of dynamic biomarkers during tumor treatment. Current methods of CTC capture often face the challenge of balancing capture rate and purity. To address these issues, we propose a microfluidic biochip based on the principle of immunoaffinity, which incorporates a herringbone microchannel and deterministic lateral displacement (DLD) technology for the capture of CTCs. By manipulating the internal structural design of the microfluidic chip, we optimized the flow field within the chip, thereby enhancing the contact frequency between cells and aptamers, and ultimately improving the capture rate. The proposed chip demonstrated a capture efficiency of approximately 91.87% for human breast cancer cells (MCF7), with a release rate of 77.5%. The relative viability of the released cells was approximately 94.08%. Notably, the specificity of the aptamers towards tumor cell surface antigens enables high-purity capture. Additionally, the use of DNA enzymes to digest aptamers facilitates the release of high-activity CTCs, offering a method to simultaneously achieve high capture rate, purity, and activity enrichment.

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