JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

Menstrual blood-derived mesenchymal stem cells differentiate into functional hepatocyte-like cells

Author(s): Xiao-zhou Mou, Jian Lin, Jin-yang Chen, Yi-fei Li, Xiao-xing Wu, Bing-yu Xiang, Cai-yun Li, Ju-ming Ma, Charlie Xiang
Affiliation(s): State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more
Corresponding email(s): majm117@sina.com, cxiang@zju.edu.cn
Key Words: Menstrual blood-derived mesenchymal stem cell (MenSC), Differentiation, Hepatocyte, Intrasplenic transplantation, Partial hepatectomy

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Xiao-zhou Mou, Jian Lin, Jin-yang Chen, Yi-fei Li, Xiao-xing Wu, Bing-yu Xiang, Cai-yun Li, Ju-ming Ma, Charlie Xiang. Menstrual blood-derived mesenchymal stem cells differentiate into functional hepatocyte-like cells[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B1300081

@article{title="Menstrual blood-derived mesenchymal stem cells differentiate into functional hepatocyte-like cells",
author="Xiao-zhou Mou, Jian Lin, Jin-yang Chen, Yi-fei Li, Xiao-xing Wu, Bing-yu Xiang, Cai-yun Li, Ju-ming Ma, Charlie Xiang",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B1300081"
}

%0 Journal Article
%T Menstrual blood-derived mesenchymal stem cells differentiate into functional hepatocyte-like cells
%A Xiao-zhou Mou
%A Jian Lin
%A Jin-yang Chen
%A Yi-fei Li
%A Xiao-xing Wu
%A Bing-yu Xiang
%A Cai-yun Li
%A Ju-ming Ma
%A Charlie Xiang
%J Journal of Zhejiang University SCIENCE B
%P 961-972
%@ 1673-1581
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/jzus.B1300081"

TY - JOUR
T1 - Menstrual blood-derived mesenchymal stem cells differentiate into functional hepatocyte-like cells
A1 - Xiao-zhou Mou
A1 - Jian Lin
A1 - Jin-yang Chen
A1 - Yi-fei Li
A1 - Xiao-xing Wu
A1 - Bing-yu Xiang
A1 - Cai-yun Li
A1 - Ju-ming Ma
A1 - Charlie Xiang
J0 - Journal of Zhejiang University Science B
SP - 961
EP - 972
%@ 1673-1581
Y1 - in press
PB - Zhejiang University Press & Springer
ER -
doi="https://doi.org/10.1631/jzus.B1300081"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Orthotopic liver transplantation (OLT) is the only proven effective treatment for both end-stage and metabolic liver diseases. hepatocyte transplantation is a promising alternative for OLT, but the lack of available donor livers has hampered its clinical application. hepatocyte-like cells (HLCs) differentiated from many multi-potential stem cells can help repair damaged liver tissue. Yet almost suitable cells currently identified for human use are difficult to harvest and involve invasive procedures. Recently, a novel mesenchymal stem cell derived from human menstrual blood (MenSC) has been discovered and obtained easily and repeatedly. In this study, we examined whether the MenSCs are able to differentiate into functional HLCs in vitro. After three weeks of incubation in hepatogenic differentiation medium containing hepatocyte growth factor (HGF), fibroblast growth factor-4 (FGF-4), and oncostain M (OSM), cuboidal HLCs were observed, and cells also expressed hepatocyte-specific marker genes including albumin (ALB), α-fetoprotein (AFP), cytokeratin 18/19 (CK18/19), and cytochrome P450 1A1/3A4 (CYP1A1/3A4). Differentiated cells further demonstrated in vitro mature hepatocyte functions such as urea synthesis, glycogen storage, and indocyanine green (ICG) uptake. After intrasplenic transplantation into mice with 2/3 partial hepatectomy, the MenSC-derived HLCs were detected in recipient livers and expressed human ALB protein. We also showed that MenSC-derived HLC transplantation could restore the serum ALB level and significantly suppressed transaminase activity of liver injury animals. In conclusion, MenSCs may serve as an ideal, easily accessible source of material for tissue engineering and cell therapy of liver tissues.

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