
CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 0000-00-00
Cited: 0
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Yefeng HU, Juan WANG, Jie XU, Zheng MA, Andreas BECHTHOLD, Xiaoping YU. Effects of S-adenosylmethionine on production of secondary metabolites in Streptomyces diastatochromogenes 1628[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2100115 @article{title="Effects of S-adenosylmethionine on production of secondary metabolites in Streptomyces diastatochromogenes 1628", %0 Journal Article TY - JOUR
S-腺苷甲硫氨酸对淀粉酶产色链霉菌1628次级代谢产物产量的影响创新点:SAM是甲基的重要供体,可为次级代谢产物提供前体,同时也可以作为信号增强次级代谢产物合成基因的表达。因此,本研究首次探究了SAM与S. diastatochromogenes1628次级代谢产物合成的相关性。 方法:以S. diastatochromogenes1628为对象,通过体外添加SAM以及在S. diastatochromogenes1628体内过表达、敲除和回补SAM编码基因metKsd,考察S. diastatochromogenes1628次级代谢产物产量的变化。 结论:由于渗透性差,外源添加SAM对S. diastatochromogenes1628合成次级代谢产物(丰加霉素和三种四烯大环内酯类抗生素:四霉素A、四霉素P和四烯菌素B)没有影响;敲除、回补和过表达基因metKsd证实增加胞内SAM可提高S. diastatochromogenes1628三种四烯大环内酯类抗生素的产量,且可促进四烯大环内酯类化合物合成关键基因的表达,而胞内SAM浓度的变化对丰加霉素的产量和及其关键基因的表达均无明显影响。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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