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On-line Access: 2024-12-30

Received: 2024-08-19

Revision Accepted: 2024-10-13

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Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B

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An integrative combination of gas-liquid-phase plasma 4 mutagenesis and high-throughput screening to enhance 5 eicosapentaenoic acid production by Schizochytrium sp.


Author(s):  Chao YU, Jialin ZHU, Jinyong WU, Xiangsong CHEN, Shuhuan LU, Xiangyu LI, Sa ZHAO, Weiwei ZHU, Min SHU, Mianbin WU, Jianming YAO

Affiliation(s):  University of Science and Technology of China, Hefei 230026, China; more

Corresponding email(s):  jmy63@ipp.ac.cn, wumb@zju.edu.cn

Key Words:  Eicosapentaenoic acid (EPA); Schizochytrium sp.; Mutagenesis; High-throughput screening; Near-infrared spectroscopy


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Abstract: 
Dietary consumption of eicosapentaenoic acid (EPA) offers diverse health benefits like regulation of blood triglycerides and prevention of cardiovascular diseases. EPA is naturally synthesized by Schizochytrium sp.; however, its low production level limit its potential for industrial application. The goal of this study was to increase EPA productivity in Schizochytrium sp. by gas-liquid-phase plasma mutagenesis (GLPP) combined with a high-throughput screening method. First, a diverse array of mutants was generated through GLPP mutagenesis. Next, the mutants with elevated EPA productivity were identified through near-infrared spectroscopy. Notably, the M7-25 mutant demonstrated the highest and most consistent EPA production. After the culture medium was optimized, the EPA titer increased from 0.45 to 1.70 g/L. Finally, a cofermentation strategy using ammonia and glucose feeding was employed, and the EPA titer reached 2.08 g/L in a 7 L fermenter. To date, this is the highest EPA titer reported in Schizochytrium sp. using mutagenesis technology, indicating its great market potential for industrial production.

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