JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

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Single-cell RNA sequencing guided reactive oxygen species scavenging hydrogel design for regeneration of osteoporotic bone

Author(s): Wei HU^1, Tianyi FEI^2, Zhichao LIU², Yude DING¹, Mengfei YU², Fan YANG¹, Feiya ZHAO¹
Affiliation(s): ¹Center for Plastic & Reconstructive Surgery, Department of Stomatology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou 310014, China ²Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou 310017, China
Corresponding email(s): Feiya ZHAO, zhaofeiya@hmc.edu.cn Fan YANG, yangfan@hmc.edu.cn
Key Words: Osteoporosis; Single-cell RNA sequencing; Reactive oxygen species (ROS); Curcumin (CCM); Bone regeneration

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Wei HU^1*, Tianyi FEI^2*, Zhichao LIU², Yude DING¹, Mengfei YU², Fan YANG¹, Feiya ZHAO¹. Single-cell RNA sequencing guided reactive oxygen species scavenging hydrogel design for regeneration of osteoporotic bone[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2500254

@article{title="Single-cell RNA sequencing guided reactive oxygen species scavenging hydrogel design for regeneration of osteoporotic bone",
author="Wei HU^1*, Tianyi FEI^2*, Zhichao LIU², Yude DING¹, Mengfei YU², Fan YANG¹, Feiya ZHAO¹",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B2500254"
}

%0 Journal Article
%T Single-cell RNA sequencing guided reactive oxygen species scavenging hydrogel design for regeneration of osteoporotic bone
%A Wei HU^1*
%A Tianyi FEI^2*
%A Zhichao LIU²
%A Yude DING¹
%A Mengfei YU²
%A Fan YANG¹
%A Feiya ZHAO¹
%J Journal of Zhejiang University SCIENCE B
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%@ 1673-1581
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/jzus.B2500254"

TY - JOUR
T1 - Single-cell RNA sequencing guided reactive oxygen species scavenging hydrogel design for regeneration of osteoporotic bone
A1 - Wei HU^1*
A1 - Tianyi FEI^2*
A1 - Zhichao LIU²
A1 - Yude DING¹
A1 - Mengfei YU²
A1 - Fan YANG¹
A1 - Feiya ZHAO¹
J0 - Journal of Zhejiang University Science B
SP -
EP -
%@ 1673-1581
Y1 - in press
PB - Zhejiang University Press & Springer
ER -
doi="https://doi.org/10.1631/jzus.B2500254"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: The pathological microenvironment of osteoporosis poses a substantial clinical challenge for bone defect regeneration. Through single-cell RNA sequencing (scRNA-seq) analysis, we identified a reactive oxygen species (ROS)-overloading osteoblast subpopulation as a critical pathological feature of osteoporotic niches. Guided by scRNA-seq analysis, we engineered a microenvironment-adaptive hydrogel system through precise integration of antioxidant curcumin-encapsulated zeolitic imidazolate framework-8 nanoparticles (CCM@ZIF-8 NPs) within photo-crosslinkable alginate methacrylate (AlgMA) hydrogel (AlgMA/CCM@ZIF-8). This engineered design exhibited dual functions: effectively scavenging ROS in bone marrow-derived mesenchymal stem cells (BMSCs) while simultaneously suppressing osteoclast differentiation. The osteo-regenerative superiority of the AlgMA/CCM@ZIF-8 nanocomposite hydrogel was conclusively demonstrated in bone defect models of osteoporotic mice. This scRNA-seq-informed engineering strategy offers a promising approach for developing pathophysiology-adapted biomaterials to promote regenerative repair in osteoporotic bone defects.

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