CLC number: R73-3
On-line Access: 2019-04-01
Received: 2018-06-07
Revision Accepted: 2018-11-15
Crosschecked: 2019-03-02
Cited: 0
Clicked: 3641
Hui Pan, Bao-hui Wang, Zhou-bin Li, Xing-guo Gong, Yong Qin, Yan Jiang, Wei-li Han. Mitochondrial superoxide anions induced by exogenous oxidative stress determine tumor cell fate: an individual cell-based study[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B1800319 @article{title="Mitochondrial superoxide anions induced by exogenous oxidative stress determine tumor cell fate: an individual cell-based study", %0 Journal Article TY - JOUR
外源性氧化应激诱导的线粒体超氧阴离子决定肿瘤细胞命运:一项基于单个细胞的研究创新点:氧自由基(ROS)涉及多种生物现象,包括有益和有害两个方面.ROS的定量检测和反应网络的评估结果令人期待.但ROS半衰期很短且反应过程很快,因此,我们通过多种手段克服了检测和评估的困难. 方法:利用改进的微流控和成像技术测定ROS水平,构建氧反应网络.通过调控线粒体胞浆Ca2+水平、线粒体Ca2+摄取、细胞内ROS自扩增以及内在凋亡途径,确定线粒体在外源氧化压力模式中扮演的角色. 结论:研究结果表明1 mmol/L H2O2引起细胞O2•−水平的快速增加,从而导致细胞氧化能力增加和还原能力降低.此外,研究还证实了内质网中储存的Ca2+是H2O2诱导的线粒体Ca2+爆发的主要来源.外源氧化压力反应涉及细胞器间Ca2+信号的传递、ROS自身扩增、线粒体功能紊乱和半胱天冬酶依赖性凋亡途径.线粒体在外源性氧化应激影响细胞命运方面发挥着关键作用. 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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