CLC number: TS253.1
On-line Access: 2020-08-04
Received: 2020-04-02
Revision Accepted: 2020-06-02
Crosschecked: 2020-07-20
Cited: 0
Clicked: 2860
Jin Zhang, Huan-Huan Li, Yi-Fan Chen, Li-Hong Chen, Hong-Gang Tang, Fan-Bin Kong, Yun-Xin Yao, Xu-Ming Liu, Qian Lan, Xiao-Fan Yu. Microencapsulation of immunoglobulin Y: optimization with response surface morphology and controlled release during simulated gastrointestinal digestion[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2000172 @article{title="Microencapsulation of immunoglobulin Y: optimization with response surface morphology and controlled release during simulated gastrointestinal digestion", %0 Journal Article TY - JOUR
免疫球蛋白Y的微胶囊化:响应面法优化及其模拟胃肠道控释创新点:以壳寡糖为涂层,采用两步法制备包埋IgY的微胶囊;采用响应面法全面优化了微胶囊的配方工艺;对IgY的体外控释进行了系统的评价. 方法:通过两步离子凝胶法制备包埋IgY的微胶囊,采用响应面法优化微胶囊配方工艺,通过包埋率、负载率、平均粒径、微观形貌、色度和球形度等指标表征微胶囊,采用模拟胃肠道消化体系体外评价IgY的释放动力学和免疫活性保持率. 结论:采用响应面法优化的微胶囊配方工艺为海藻酸钠浓度1.56%(15.6 g/L)、壳寡糖浓度0.61%(6.1 g/L)和IgY/海藻酸钠比率62.44%(质量比),由此制备的微胶囊包埋率达65.19%,负载率达33.75%,平均粒径为588.75 μm.壳寡糖涂层通过填充效应和静电相互作用使微胶囊表面更加光滑和连续,从而显著降低了模拟胃肠道消化过程中IgY的释放速率.微胶囊中IgY的模拟胃部控释和模拟肠道控释分别符合零级动力学方程和一级动力学方程(R2>0.99).微胶囊在模拟胃肠道消化4 h后的IgY免疫活性保持率达84.37%,远高于未进行微胶囊化保护的IgY(5.33%). 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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