CLC number:
On-line Access: 2021-05-07
Received: 2020-08-07
Revision Accepted: 2020-12-23
Crosschecked: 0000-00-00
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Citations: Bibtex RefMan EndNote GB/T7714
https://orcid.org/0000-0003-0947-7086
Guan WANG, Mingyue HAO, Qiong LIU, Yanlong JIANG, Haibin HUANG, Guilian YANG, Chunfeng WANG. Protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in HUVEC cells[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2000441 @article{title="Protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in HUVEC cells", %0 Journal Article TY - JOUR
重组植物乳杆菌对H2O2诱导HUVEC细胞产生氧化应激损伤的保护作用创新点:成功构建非抗性筛选标记的新功能植物乳杆菌NC8-pSIP409-alr-ACEIP,其具有安全、方便、无毒副作用,且以食疗方式达到降血压的优点。研究NC8-pSIP409-alr-ACEIP对相关蛋白血管紧张素II(AngII)、血管紧张素转化酶2(ACE2)、B淋巴细胞瘤-2(Bcl-2)、BCL2关联X蛋白(Bax)与氧化应激相关因子的变化情况,为阐明NC8-pSIP409-alr-ACEIP保护血管内皮细胞损伤的作用机制提供了基础的理论依据。 方法:首次构建以双基因标记的非抗性筛选锚定为表达载体的新功能型植物乳杆菌(NC8-pSIP409-alr-ACEIP),使用不同浓度的过氧化氢(H2O2)诱导人脐静脉内皮细胞(HUVEC)细胞,构建氧化应激损伤细胞模型(Hy-HUVEC),通过MTT、流式细胞术等方法检测Hy-HUVEC的凋亡,通过蛋白质印迹法(western blot)检测AngII、ACE2、诱导型一氧化氮合酶(iNOS)、尼克酰胺腺嘌呤二核苷酸磷酸氧化酶(gp91phox)、Bcl-2和Bax的蛋白表达情况,同时检测氧化应激相应指标活性氧(ROS)、线粒体膜电位(MMP)、丙二醛(MDA)和超氧化物歧化酶(SOD)的变化情况。 结论:NC8-pSIP409-alr-ACEIP对H2O2诱导的HUVEC细胞氧化应激损伤具有保护作用,其作用机制与增强抗氧化能力和降低由线粒体凋亡通路引发的细胞凋亡有关。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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