CLC number:
On-line Access: 2021-11-16
Received: 2021-04-20
Revision Accepted: 2021-06-15
Crosschecked: 0000-00-00
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Citations: Bibtex RefMan EndNote GB/T7714
Jianwei CHANG, Chong XIE, Pei WANG, Zhenxin GU, Yongbin HAN, Runqiang YANG. Red light enhances folate accumulation in wheat seedlings[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2100266 @article{title="Red light enhances folate accumulation in wheat seedlings", %0 Journal Article TY - JOUR
红光促进小麦芽苗中叶酸积累创新点:在小麦芽苗生长过程中使用不同的光谱(白色、红色、蓝色、绿色和黄色)处理,发现了刺激叶酸合成的最佳光谱和强度,通过研究叶酸分布、叶酸生物合成中前体、酶和基因表达,进一步阐明了红光调控小麦芽苗叶酸积累的机制。 方法:小麦种子在25 °C下避光发芽2天后,第3天开始用30 µmol/(m2?s)的LED白光、红光(655 nm峰值)、蓝光(445 nm峰值)、绿光(520 nm峰值)和黄光(595 nm峰值)照射培养4天。光周期为16小时光照和8小时黑暗。在光照强度实验中,光照强度设置为0、15、30、45或60 µmol/(m2·s)。通过测定叶酸含量找出刺激叶酸合成的最佳光谱和强度。进一步研究最佳光照下小麦芽苗中叶酸分布、叶酸生物合成中前体、酶和基因表达。 结论:发芽和光处理增加了小麦芽苗叶酸的积累,30 µmol/(m2·s)红光照射下叶酸积累最多。红光促进小麦芽苗叶酸的积累主要表现在叶片中,尤其增加5-CH3-THF含量。此外,红光上调了叶酸合成相关基因表达,导致叶酸合成关键酶活性和前体含量的增加。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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