CLC number:
On-line Access: 2022-04-11
Received: 2021-08-02
Revision Accepted: 2021-11-03
Crosschecked: 2022-04-19
Cited: 0
Clicked: 1999
Citations: Bibtex RefMan EndNote GB/T7714
Zilan XIAO, Jianping DENG, Xiaojun ZHOU, Liyan ZHU, Xiaochan HE, Jingwu ZHENG, Deping GUO, Jingze ZHANG. Shoot rot of Zizania latifolia and the first record of its pathogen Pantoea ananatis in China[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2100682 @article{title="Shoot rot of Zizania latifolia and the first record of its pathogen Pantoea ananatis in China", %0 Journal Article TY - JOUR
茭白鞘腐病及其病原菌菠萝泛菌在中国的首次报道创新点:首次明确了引起茭白鞘腐病的病原菌是菠萝泛菌,揭示了菠萝泛菌在茭白组织中与寄主互作的超微结构特性,并研发了特异性引物可用于对引起该病害的病原菌进行早期检测和病害诊断。 方法:通过病原菌16S rRNA序列及结合多基因(atpD、gyrB、infB和rpoB)序列的系统发育分析,以及致病性试验结果,进行了病原菌鉴定;通过扫描电子显微镜,对病原菌与寄主互作的超微结构特征进行观察;分析了病原菌属内种间同源基因的碱基差异,设计出检测病原菌的专化性引物,并进行验证。 结论:本文系统观察和描述了发生在浙江地区茭白鞘腐病的症状,并通过系统发育分析和致病试验首次鉴定了引起引茭白鞘腐病的病原菌是菠萝泛菌。超微结构观察认为,菠萝泛菌主要定殖于寄主叶鞘中的维管束组织,形成生物膜,是涉及致病性;并通过导管分子多孔板在导管中传播。设计的病原菌专化性引物对pagyrB-F/R可用于对引起该病害的病原菌进行早期检测和病害诊断。这为该病害的防治提供了病原菌种的重要信息。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
Reference[1]AnnPJ, HuangJH, WangIT, et al., 2006. Pythiogeton zizaniae, a new species causing basal stalk rot of water bamboo in Taiwan. Mycologia, 98(1):116-120. [2]AsselinJAE, BonaseraJM, BeerSV, 2016. PCR primers for detection of Pantoea ananatis, Burkholderia spp. and Enterobacter sp. from onion. Plant Dis, 100(4):836-846. [3]AzadHR, HolmesGJ, CookseyDA, 2000. A new leaf blotch disease of sudangrass caused by Pantoea ananas and Pantoea stewartii. Plant Dis, 84(9):973-979. [4]BradyC, CleenwerckI, VenterS, et al., 2008. Phylogeny and identification of Pantoea species associated with plants, humans and the natural environment based on multilocus sequence analysis (MLSA). Syst Appl Microbiol, 31(6-8):447-460. [5]BraunEJ, 1982. Ultrastructural investigation of resistant and susceptible maize inbreds infected with Erwinia Stewartii. Phytopathology, 72(1):159-166. [6]CarrEA, BonaseraJM, ZaidAM, et al., 2010. First report of bulb disease of onion caused by Pantoea ananatis in New York. Plant Dis, 94(7):916. [7]CastiblancoLF, SundinGW, 2016. New insights on molecular regulation of biofilm formation in plant-associated bacteria. J Integr Plant Biol, 58(4):362-372. [8]CotaLV, CostaRV, SilvaDD, et al., 2010. First report of pathogenicity of Pantoea ananatis in sorghum (Sorghum bicolor) in Brazil. Austral Plant Dis Notes, 5(1):120-122. [9]CotherEJ, ReinkeR, McKenzieC, et al., 2004. An unusual stem necrosis of rice caused by Pantoea ananas and the first record of this pathogen on rice in Australia. Austral Plant Pathol, 33(4):495-503. [10]CoutinhoTA, VenterSN, 2009. Pantoea ananatis: an unconventional plant pathogen. Mol Plant Pathol, 10(3):325-335. [11]DarribaD, TaboadaGL, DoalloR, et al., 2012. jModelTest 2: more models, new heuristics and parallel computing. Nat Methods, 9(8):772. [12]de MaayerP, ChanWY, RubagottiE, et al., 2014. Analysis of the Pantoea ananatis pan-genome reveals factors underlying its ability to colonize and interact with plant, insect and vertebrate hosts. BMC Genomics, 15:404. [13]DengJP, ZhangJZ, HuMH, 2015. Occurrence regularity and control of Jiaobai rust in Dayang Town, Jinyun County. J Chang Jiang Veget, (17):51-53 (in Chinese). [14]FigueiredoJEF, Paccola-MeirellesLD, 2012. Simple, rapid and accurate PCR-based detection of Pantoea ananatis in maize, sorghum and Digitaria sp. J Plant Pathol, 94(3):663-667. [15]GitaitisR, WalcottR, CulpepperS, et al., 2002. Recovery of Pantoea ananatis, causal agent of center rot of onion, from weeds and crops in Georgia, USA. Crop Prot, 21(10):983-989. [16]GitaitisRD, GayJD, 1997. First report of a leaf blight, seed stalk rot, and bulb decay of onion by Pantoea ananas in Georgia. Plant Dis, 81(9):1096. [17]HallTA, 1999. Bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser, 41:95-98. [18]KatohK, StandleyDM, 2013. MAFFT multiple sequence alignment software Version 7: improvements in performance and usability. Mol Biol Evol, 30(4):772-780. [19]KoutsoudisMD, TsaltasD, MinogueTD, et al., 2006. Quorum-sensing regulation governs bacterial adhesion, biofilm development, and host colonization in Pantoea stewartii subspecies stewartii. Proc Natl Acad Sci USA, 103(15):5983-5988. [20]KumarS, StecherG, TamuraK, 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for bigger datasets. Mol Biol Evol, 33(7):1870-1874. [21]LaneDJ, 1991. 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (Eds.), Nucleic Acid Techniques in Bacterial Systematics. Wiley, Chichester, p.115-175. [22]LaneDJ, PaceB, OlsenGJ, et al., 1985. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci USA, 82(20):6955-6959. [23]LiXL, OjaghianMR, ZhangJZ, et al., 2017. A new species of Scopulariopsis and its synergistic effect on pathogenicity of Verticillium dahliae on cotton plants. Microbiol Res, 201:12-20. [24]NisikadoY, 1929. Studies on the Helminthosporium diseases of Gramineae in Japan. Ber Ohara Inst Landw Forsch, 4(1):111-126. [25]Paccola-MeirellesLD, FerreiraAS, MeirellesWF, et al., 2001. Detection of a bacterium associated with a leaf spot disease of maize in Brazil. J Phytopathol, 149(5):275-279. [26]Sheibani-TezerjiR, NaveedM, JehlMA, et al., 2015. The genomes of closely related Pantoea ananatis maize seed endophytes having different effects on the host plant differ in secretion system genes and mobile genetic elements. Front Microbiol, 6:440. [27]ShoemakerRA, 2006. Nomenclature of Drechslera and Bipolaris, grass parasites segregated from ‘Helminthosporium’. Can J Plant Pathol, 28(S1):S212-S220. [28]SilvestroD, MichalakI, 2012. raxmlGUI: a graphical front-end for RAxML. Org Divers Evol, 12(4):335-337. [29]StallRE, AlexanderLJ, HallCB, 1969. Effect of tobacco mosaic virus and bacterial infections on occurrence of graywall of tomato. Proc Fla State Hortic Soc, 82:157-161. [30]TalaveraG, CastresanaJ, 2007. Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst Biol, 56(4):564-577. [31]TurnerS, PryerKM, MiaoVPW, alet, 1999. Investigating deep phylogenetic relationships among cyanobacteria and plastids by small subunit rRNA sequence analysis. J Eukaryot Microbiol, 46(4):327-338. [32]TysonGE, StojanovicBJ, KuklinskiRF, et al., 1985. Scanning electron microscopy of Pierce’s disease bacterium in petiolar xylem of grape leaves. Phytopathology, 75(3):264-269. [33]VaidyaG, LohmanDJ, MeierR, 2011. SequenceMatrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics, 27(2):171-180. [34]WellsJM, ShengWS, CeponisMJ, et al., 1987. Isolation and characterization of strains of Erwinia ananas from honeydew melons. Phytopathology, 77(3):511-514. [35]XiaoZL, HydeKD, ZhangJZ, 2015. Synonymy of two species of Bipolaris from aquatic crops of Poaceae. Mycotaxon, 130(1):131-143. [36]XuXW, WaltersC, AntolinMF, et al., 2009. Phylogeny and biogeography of the eastern Asian-North American disjunct wild-rice genus (Zizania L., Poaceae). Mol Phylogenet Evol, 55(3):1008-1017. [37]ZhangF, LiXL, ZhuSJ, et al., 2018. Biocontrol potential of Paenibacillus polymyxa against Verticillium dahliae infecting cotton plants. Biol Control, 127:70-77. [38]ZhangJZ, ChuFQ, GuoDP, et al., 2012. Cytology and ultrastructure of interactions between Ustilago esculenta and Zizania latifolia. Mycol Prog, 11(2):499-508. [39]ZhangJZ, ChuFQ, GuoDP, et al., 2014. The vacuoles containing multivesicular bodies: a new observation in interaction between Ustilago esculenta and Zizania latifolia. Eur J Plant Pathol, 138(1):79-91. Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou
310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE |
Open peer comments: Debate/Discuss/Question/Opinion
<1>