CLC number:
On-line Access: 2022-06-08
Received: 2021-12-13
Revision Accepted: 2022-02-04
Crosschecked: 2022-06-08
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Citations: Bibtex RefMan EndNote GB/T7714
https://orcid.org/0000-0002-6047-4916
https://orcid.org/0000-0001-7052-4967
Weidi ZHANG, Wenzhi REN, Dongxu HAN, Guokun ZHAO, Haoqi WANG, Haixiang GUO, Yi ZHENG, Zhonghao JI, Wei GAO, Bao YUAN. LncRNA-m18as1 competitively binds with miR-18a-5p to regulate follicle-stimulating hormone secretion through the Smad2/3 pathway in rat primary pituitary cells[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2101052 @article{title="LncRNA-m18as1 competitively binds with miR-18a-5p to regulate follicle-stimulating hormone secretion through the Smad2/3 pathway in rat primary pituitary cells", %0 Journal Article TY - JOUR
LncRNA-m18as1竞争性结合miR-18a-5p通过Smad2/3通路调控大鼠原代腺垂体细胞FSH分泌1吉林大学动物科学学院,实验动物系,中国长春市,130062 2吉林大学吉林省模型动物工程研究中心,中国长春市,130062 目的:探索长链非编码RNA(lncRNA)-微小RNA(miRNA)-信使RNA(mRNA)网络对促卵泡素(FSH)合成与分泌的作用,为调控FSH的分子机制提供理论基础。 创新点:筛选出了一个新的lncRNA作为研究对象;首次鉴定并分析了该lncRNA对FSH合成与分泌的调控作用;确定了该lncRNA调控FSH合成与分泌的机制。 方法:我们通过逆转录定量聚合酶链反应(RT-qPCR)筛选出了一个新的lncRNA,并根据功能将其最终命名为lncRNA-m18as1。经过敲降或过表达lncRNA-m18as1后,我们采用RT-qPCR与酶联免疫吸附剂测定(ELISA)分析了lncRNA-m18as1对FshβmRNA以及FSH分泌的调控作用。我们预测并确定了lncRNA-m18as1发挥作用的lncRNAm18as1/miR-18a-5p/Smad2轴。我们使用RNA结合蛋白免疫沉淀测定-逆转录定量聚合酶链反应(RIP-qPCR)和/或双荧光素酶报告分析方法分析了miR-18a-5p与lncRNA-m18as1、Smad2的靶向关系。此外,我们使用RT-qPCR和蛋白质印迹法(western blot)分析了lncRNA-m18as1和miR-18a-5p在轴中对向下游因子的调控作用,同时通过荧光原位杂交技术(FISH)观察了lncRNA-m18as1以及miR-18a-5p在细胞核与细胞质中的分布。 结论:研究结果表明在大鼠腺垂体中高表达且阶段性表达的lncRNA-m18as1促进FshβmRNA的表达与FSH的分泌。进一步的分子机制研究表明lncRNA-m18as1竞争性结合miR-18a-5p调控Smad2蛋白的表达,进而调控FSH的合成与分泌。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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