CLC number:
On-line Access: 2023-02-05
Received: 2022-04-11
Revision Accepted: 2022-09-25
Crosschecked: 2023-02-09
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Citations: Bibtex RefMan EndNote GB/T7714
Jianjia HUANG, Yuman BAI, Wenting XIE, Rongmei WANG, Wenyue QIU, Shuilian ZHOU, Zhaoxin TANG, Jianzhao LIAO, Rongsheng SU. Lycium barbarum polysaccharides ameliorate canine acute liver injury by reducing oxidative stress, protecting mitochondrial function, and regulating metabolic pathways[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2200213 @article{title="Lycium barbarum polysaccharides ameliorate canine acute liver injury by reducing oxidative stress, protecting mitochondrial function, and regulating metabolic pathways", %0 Journal Article TY - JOUR
枸杞多糖通过降低氧化应激、保护线粒体功能和调节代谢途径改善犬急性肝损伤1华南农业大学兽医学院,中国广州,510642 2广州医药研究总院有限公司,中国广州,510240 3韶关大学生物与农业学院,中国韶关,512005 概要:急性肝损伤的发展可导致肝硬化、肝衰竭,甚至肝癌,但目前尚无有效的治疗方法。本研究旨在探讨枸杞多糖(LBPs)对四氯化碳(CCl4)诱导的急性肝损伤的保护作用及其治疗机制。为了建立急性肝损伤模型,以1 mL/kg的剂量对实验犬进行CCl4溶液的腹腔注射。然后给治疗组的实验犬喂食枸杞多糖(20 mg/kg)。微观结果显示,CCl4诱导的肝脏结构损伤、过度纤维化和线粒体密度降低都可以通过给予LBPs得到改善。LBPs可以通过抑制Kelch样ECH相关蛋白1(Keap1),促进蛋白(p62)、核因子E2相关因子2(Nrf2)和Nrf2下游的II期解毒基因和蛋白的产生,以及恢复过氧化氢酶(CAT)等抗氧化酶的活性,恢复并增加肝脏的抗氧化能力。LBPs还可以增强线粒体呼吸能力,提高组织ATP水平,以及重新激活呼吸链复合体I-V以减轻线粒体的损伤。血清代谢组学结果显示,LBPs对急性肝损伤的治疗作用主要通过控制脂质代谢途径实现。9-羟基十八碳二烯酸(9-HODE)、溶血磷脂酰胆碱(LPC)和磷脂酰乙醇胺(PE)可能是急性肝损伤的潜在标志物。这项研究证实,LBPs是一种有效的保肝药物,可以通过降低氧化应激、修复线粒体损伤和调节代谢途径来治疗急性肝损伤。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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