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On-line Access: 2023-03-10

Received: 2022-04-17

Revision Accepted: 2022-09-06

Crosschecked: 2023-03-13

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Yanran WU


Sen LI


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Journal of Zhejiang University SCIENCE B

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Aerobic glycolysis in colon cancer is repressed by naringin via the HIF1Α pathway

Author(s):  Guangtao PAN, Ping ZHANG, Aiying CHEN, Yu DENG, Zhen ZHANG, Han LU, Aoxun ZHU, Cong ZHOU, Yanran WU, Sen LI

Affiliation(s):  Yancheng TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Yancheng 224000, China; more

Corresponding email(s):  lisentome728@hotmail.com, wuyanran196512@163.com

Key Words:  Colon cancer; Naringin; Hypoxia inducible factor-1α (HIF1Α); Enolase 2 (ENO2); Glycolysis; Metabolic reprogramming

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Guangtao PAN, Ping ZHANG, Aiying CHEN, Yu DENG, Zhen ZHANG, Han LU, Aoxun ZHU, Cong ZHOU, Yanran WU, Sen LI. Aerobic glycolysis in colon cancer is repressed by naringin via the HIF1Α pathway[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2200221

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author="Guangtao PAN, Ping ZHANG, Aiying CHEN, Yu DENG, Zhen ZHANG, Han LU, Aoxun ZHU, Cong ZHOU, Yanran WU, Sen LI",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Aerobic glycolysis in colon cancer is repressed by naringin via the HIF1Α pathway
%A Guangtao PAN
%A Aiying CHEN
%A Han LU
%A Aoxun ZHU
%A Cong ZHOU
%A Yanran WU
%A Sen LI
%J Journal of Zhejiang University SCIENCE B
%P 221-231
%@ 1673-1581
%D in press
%I Zhejiang University Press & Springer

T1 - Aerobic glycolysis in colon cancer is repressed by naringin via the HIF1Α pathway
A1 - Guangtao PAN
A1 - Ping ZHANG
A1 - Aiying CHEN
A1 - Yu DENG
A1 - Zhen ZHANG
A1 - Han LU
A1 - Aoxun ZHU
A1 - Cong ZHOU
A1 - Yanran WU
A1 - Sen LI
J0 - Journal of Zhejiang University Science B
SP - 221
EP - 231
%@ 1673-1581
Y1 - in press
PB - Zhejiang University Press & Springer
ER -

Metabolic reprogramming is a common phenomenon in cancer, with aerobic glycolysis being one of its important characteristics. Hypoxia-inducible factor-1α (HIF1Α) is thought to play an important role in aerobic glycolysis. Meanwhile, naringin is a natural flavanone glycoside derived from grapefruits and many other citrus fruits. In this work, we identified glycolytic genes related to HIF1Α by analyzing the colon cancer database. The analysis of extracellular acidification rate and cell function verified the regulatory effects of HIF1Α overexpression on glycolysis, and the proliferation and migration of colon cancer cells. Moreover, naringin was used as an inhibitor of colon cancer cells to illustrate its effect on HIF1Α function. The results showed that the HIF1Α and enolase 2 (ENO2) levels in colon cancer tissues were highly correlated, and their high expression indicated a poor prognosis for colon cancer patients. Mechanistically, HIF1Α directly binds to the DNA promoter region and upregulates the transcription of ENO2; ectopic expression of ENO2 increased aerobic glycolysis in colon cancer cells. Most importantly, we found that the appropriate concentration of naringin inhibited the transcriptional activity of HIF1Α, which in turn decreased aerobic glycolysis in colon cancer cells. Generally, naringin reduces glycolysis in colon cancer cells by reducing the transcriptional activity of HIF1Α and the proliferation and invasion of colon cancer cells. This study helps to elucidate the relationship between colon cancer progression and glucose metabolism, and demonstrates the efficacy of naringin in the treatment of colon cancer.


潘广涛1,张平2,陈爱莹3, 邓宇2,张臻1,卢翰1,朱奥旬1,周聪2,吴嫣然4,李森5


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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