CLC number:
On-line Access: 2023-11-14
Received: 2022-12-14
Revision Accepted: 2023-04-16
Crosschecked: 2023-11-15
Cited: 0
Clicked: 571
Muhammad Zahid FAROOQ, Xinkai WANG, Xianghua YAN. Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2200621 @article{title="Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens", %0 Journal Article TY - JOUR
Aeriscardovia aeriphila对肉鸡生长性能、抗氧化功能、免疫应答和肠道菌群的影响1华中农业大学动物科学技术学院,农业微生物资源发掘与利用全国重点实验室,湖北洪山实验室,动物育种与健康养殖前沿科学中心,中国武汉市,430070 2巴基斯坦兽医和动物科学大学(Jhang校区)动物科学系,拉合尔54000,巴基斯坦 摘要:Aeriscardovia aeriphila,又名Bifidobacterium aerophilum,是从猪盲肠内容物中分离出的一种双歧杆菌。A. aeriphila在预防肠道感染、降低胆固醇水平和刺激免疫系统方面具有重要作用。本研究首先从肉鸡十二指肠肠道内容物中分离出一株菌,经16S 测序鉴定为 A. aeriphila,然后我们评估了A. aeriphila对商用肉鸡生长性能、抗氧化功能、免疫功能和肠道菌群的影响。实验对21日龄的实验鸡进行了为期3周的灌服A. aeriphila(1×109 CFU/mL)处理。研究结果显示:A. aeriphila处理显著增加了鸡的平均日增重,降低了饲料转化率(P<0.001)。A. aeriphila处理后,血清生长激素(GH)和胰岛素样生长因子1(IGF-1)水平显著升高(P<0.05)。A. aeriphila处理降低了血尿素氮和天门冬氨酸氨基转移酶水平,增加了血糖和肌酐水平。同时,A. aeriphila处理显著提高了血清中抗氧化酶水平(包括过氧化氢酶(P<0.01)、超氧化物歧化酶(P<0.001)和谷胱甘肽过氧化物酶(P<0.05))及总抗氧化能力(P<0.05)。A. aeriphila处理显著增加了血清免疫球蛋白A(IgA)(P<0.05)、IgG(P<0.01)、IgM(P<0.05)、白细胞介素-1(IL-1)(P<0.05)、IL-4(P<0.05)和IL-10(P<0.05)的水平。与对照组相比,A. aeriphila组肉鸡在十二指肠(P<0.01)、空肠(P<0.001)和盲肠(P<0.001)中的分泌型免疫球蛋白A(SIgA)水平显著提高。在肠道黏膜(包括十二指肠、空肠和盲肠)中,A. aeriphila组鸡的IL-10(P<0.05)、IL-4(P<0.001)和NF-κB(P<0.001)信使RNA(mRNA)相对表达水平高于对照组。肠道微生物门水平分析显示,两组的差异菌群主要是厚壁菌门,其次是拟杆菌门。此外,A. aeriphila可提高肠道中Phascolarctobacterium和Barnesiella的丰度。综上所述,灌服A. aeriphila可改善肉鸡的生长性能、血清抗氧化能力、免疫调节和肠道健康。因此,本研究结果可以为A. aeriphila在家禽生产中的应用提供理论依据。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
Reference[1]AbdulrahimSM,HaddadinMSY,HashlamounEAR,et al.,1996.The influence ofLactobacillusacidophilus and bacitracin on layer performance of chickens and cholesterol content of plasma and egg yolk.Br Poultry Sci,37(2):341-346. [2]AdalsteinsdottirSA,MagnusdottirOK,HalldorssonTI,et al.,2018.Towards an individualized nutrition treatment: role of the gastrointestinal microbiome in the interplay between diet and obesity.Curr Obes Rep,7(4):289-293. [3]AlaqabyARA,GlaskovichAA,KapitonovaEA,et al.,2014.Study the effect of using probiotic (vetlactoflorum) on some of biochemical and immunological parameters of broiler chickens.Basrah J Vet Res,13(1):166-179. [4]BaderJ,AlbinA,StahlU,2012.Spore-forming bacteria and their utilisation as probiotics.Benefic Microbes,3(1):67-75. [5]BaiSP,WuAM,DingXM,et al.,2013.Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens.Poult Sci,92(3):663-670. [6]BeckmanBR,LarsenDA,MoriyamaS,et al.,1998.Insulin-like growth factor-I and environmental modulation of growth during smoltification of spring chinook salmon (Oncorhynchus tshawytscha).Gen Comp Endocrinol,109(3):325-335. [7]BianXF,WallstromG,DavisA,et al.,2016.Immunoproteomic profiling of antiviral antibodies in new-onset type 1 diabetes using protein arrays.Diabetes,65(1):285-296. [8]BirbrairA,ZhangT,WangZM,et al.,2013.Role of pericytes in skeletal muscle regeneration and fat accumulation.Stem Cells Dev,22(16):2298-2314. [9]BuntynJO,SchmidtTB,NisbetDJ,et al.,2016.The role of direct-fed microbials in conventional livestock production.Annu Rev Anim Biosci,4:335-355. [10]CaoGT,DaiB,WangKL,et al.,2019.Bacillus licheniformis, a potential probiotic, inhibits obesity by modulating colonic microflora in C57BL/6J mice model.J Appl Microbiol,127(3):880-888. [11]CaporasoJG,KuczynskiJ,StombaughJ,et al.,2010.QIIME allows analysis of high-throughput community sequencing data.Nat Methods,7(5):335-336. [12]ChenYC,YuYH,2020.Bacillus licheniformis-fermented products improve growth performance and the fecal microbiota community in broilers.Poult Sci,99(3):1432-1443. [13]ChengGY,HaoHH,XieSY,et al.,2014.Antibiotic alternatives: the substitution of antibiotics in animal husbandry?Front Microbiol,5:217. [14]DasHK,MedhiAK,IslamM,2005.Effect of probiotics on certain blood parameters and carcass characteristics of broiler chicken.Indian J Poult Sci,40(1):83-86. [15]de BoeverS,VangestelC,de BackerP,et al.,2008.Identification and validation of housekeeping genes as internal control for gene expression in an intravenous LPS inflammation model in chickens.Vet Immunol Immunopathol,122(3-4):312-317. [16]DjouvinovD,BoichevaS,SimeonovaT,et al.,2005.Effect of feeding Lactina® probiotic on performance, some blood parameters and caecal microflora of mule ducklings.Trakia J Sci,3(2):22-28. [17]EdgarRC,2013.UPARSE: highly accurate OTU sequences from microbial amplicon reads.Nat Methods,10(10):996-998. [18]EdgarRC,HaasBJ,ClementeJC,et al.,2011.UCHIME improves sensitivity and speed of chimera detection.Bioinformatics,27(16):2194-2200. [19]FerrarioC,MilaniC,MancabelliL,et al.,2016.Modulation of theeps-ome transcription ofBifidobacteria through simulation of human intestinal environment.FEMS Microbiol Ecol,92(4):fiw056. [20]García-CayuelaT,KoranyAM,BustosI,et al.,2014.Adhesion abilities of dairyLactobacillusplantarum strains showing an aggregation phenotype.Food Res Int,57:44-50. [21]HillC,GuarnerF,ReidG,et al.,2014.Expert consensus document: the international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic.Nat Rev Gastroenterol Hepatol,11(8):506-514. [22]HirayamaH,SuzukiY,AbeM,et al.,2011.Methylothermus subterraneus sp. Nov., a moderately thermophilic methanotroph isolated from a terrestrial subsurface hot aquifer.Int J Syst Evol Microbiol,61(Pt 11):2646-2653. [23]KhaliqueA,ZengD,ShoaibM,et al.,2020.Probiotics mitigating subclinical necrotic enteritis (SNE) as potential alternatives to antibiotics in poultry.AMB Express,10:50. [24]KhanS,RobertsJ,WuSB,2017.Reference gene selection for gene expression study in shell gland and spleen of laying hens challenged with infectious bronchitis virus.Sci Rep,7:14271. [25]LammersA,WielandWH,KruijtL,et al.,2010.Successive immunoglobulin and cytokine expression in the small intestine of juvenile chicken.Dev Comp Immunol,34(12):1254-1262. [26]LinLH,KeFR,ZhanTT,et al.,2014.Effects ofBacilluscoagulans on performance, serum biochemical indices and antioxidant function of yellow broilers.Chin J Anim Nutr,26(12):3806-3813(in Chinese). [27]LiuJB,YanHL,ZhangY,et al.,2020.Effects of stale maize on growth performance, immunity, intestinal morphology and antioxidant capacity in broilers.Asian-Australas J Anim Sci,33(4):605-614. [28]LiuSQ,WangLY,LiuGH,et al.,2018.Leucine alters immunoglobulin a secretion and inflammatory cytokine expression induced by lipopolysaccharide via the nuclear factor-κB pathway in intestine of chicken embryos.Animal,12(9):1903-1911. [29]LiuX,LiSR,WangZY,et al.,2018.Effects of dietary lipids and clostridium butyricum on chicken volatile flavour compounds.Indian J Anim Res,52(8):1167-1173. [30]LiuXL,YanH,LvL,et al.,2012.Growth performance and meat quality of broiler chickens supplemented withBacilluslicheniformis in drinking water.Asian-Australas J Anim Sci,25(5):682-689. [31]LivakKJ,SchmittgenTD,2001.Analysis of relative gene expression data using real-time quantitative PCR and the2-∆∆CTmethod.Methods,25(4):402-408. [32]LivingstonML,CowiesonAJ,CrespoR,et al.,2020.Effect of broiler genetics, age, and gender on performance and blood chemistry.Heliyon,6(7):e04400. [33]LokapirnasariWP,al ArifA,SoeharsonoS,et al.,2019.Improves in external and internal egg quality of Japanese quail (Coturnix coturnix japonica) by giving lactic acid bacteria as alternative antibiotic growth promoter.Iran J Microbiol,11(5):406-411. [34]LouisP,FlintHJ,2009.Diversity, metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine.FEMS Microbiol Lett,294(1):1-8. [35]MagočT,SalzbergSL,2011.FLASH: fast length adjustment of short reads to improve genome assemblies.Bioinformatics,27(21):2957-2963. [36]MassacciFR,LovitoC,TofaniS,et al.,2019.DietarySaccharomyces cerevisiae boulardii CNCM I-1079 positively affects performance and intestinal ecosystem in broilers during aCampylobacter jejuni infection.Microorganisms,7(12):596. [37]MehdiY,Létourneau-MontminyMP,GaucherML,et al.,2018.Use of antibiotics in broiler production: global impacts and alternatives.Anim Nutr,4(2):170-178. [38]MichaelidouK,TzovarasA,MissitzisI,et al.,2013.The expression of the CEACAM19 gene, a novel member of the CEA family, is associated with breast cancer progression.Int J Oncol,42(5):1770-1777. [39]MohanB,KadirvelR,BhaskaranM,et al.,1995.Effect of probiotic supplementation on serum/yolk cholesterol and on egg shell thickness in layers.Br Poultry Sci,36(5):799-803. [40]MohanB,KadirvelR,NatarajanA,et al.,1996.Effect of probiotic supplementation on growth, nitrogen utilisation and serum cholesterol in broilers.Br Poultry Sci,37(2):395-401. [41]PandaAK,RajuMVLN,RamaRSV,et al.,2005.The influence of supplementation ofLactobacillussporogenes on the performance of broilers.Indian J Anim Nutr,22(1):37-40. [42]PandaAK,RaoSVR,RajuMVLN,et al.,2006.Dietary supplementation ofLactobacillus sporogenes on performance and serum biochemico-lipid profile of broiler chickens.J Poult Sci,43(3):235-240. [43]RussellDA,RossRP,FitzgeraldGF,et al.,2011.Metabolic activities and probiotic potential of bifidobacteria.Int J Food Microbiol,149(1):88-105. [44]ScanesCG,2009.Perspectives on the endocrinology of poultry growth and metabolism.Gen Comp Endocrinol,163(1-2):24-32. [45]SuraiPF,KochishII,FisininVI,2017.Antioxidant systems in poultry biology: nutritional modulation of vitagenes.Eur Poult Sci,81:1-21. [46]ThapaBR,WaliaA,2007.Liver function tests and their interpretation.Indian J Pediatr,74(7):663-671. [47]VitalM,HoweAC,TiedjeJM,2014.Revealing the bacterial butyrate synthesis pathways by analyzing (meta) genomic data.mBio,5(2):e00889-14. [48]WangX.,HuY.,ZhuX,et al.,2023.Bacteroides-derived isovaleric acid enhances mucosal immunity by facilitating intestinal IgA response in broilers.J Animal Sci Biotechnol,14:4. [49]ZhengJS,WittouckS,SalvettiE,et al.,2020.A taxonomic note on the genusLactobacillus: description of 23 novel genera, emended description of the genusLactobacillus Beijerinck 1901, and union ofLactobacillaceae andLeuconostocaceae.Int J Syst Evol Microbiol,70(4):2782-2858. Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou
310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE |
Open peer comments: Debate/Discuss/Question/Opinion
<1>