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On-line Access: 2023-09-20

Received: 2023-03-09

Revision Accepted: 2023-08-29

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Journal of Zhejiang University SCIENCE B

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RAD51B-AS1 promotes the malignant biological behavior of ovarian cancer through upregulation of RAD51B


Author(s):  Xinyi WEI, Conghui WANG, Sangsang TANG, Qian YANG, Zhangjin SHEN, Jiawei ZHU, Xiaodong CHENG, Xinyu WANG, Xing XIE, Junfen XU, Weiguo LU

Affiliation(s):  Women’s Reproductive Health Laboratory of Zhejiang Province, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China; more

Corresponding email(s):  xjfzu@zju.edu.cn, lbwg@zju.edu.cn

Key Words:  Ovarian cancer; LncRNA; Metastasis; Anoikis


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Xinyi WEI, Conghui WANG, Sangsang TANG, Qian YANG, Zhangjin SHEN, Jiawei ZHU, Xiaodong CHENG, Xinyu WANG, Xing XIE, Junfen XU, Weiguo LU. RAD51B-AS1 promotes the malignant biological behavior of ovarian cancer through upregulation of RAD51B[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2300154

@article{title="RAD51B-AS1 promotes the malignant biological behavior of ovarian cancer through upregulation of RAD51B",
author="Xinyi WEI, Conghui WANG, Sangsang TANG, Qian YANG, Zhangjin SHEN, Jiawei ZHU, Xiaodong CHENG, Xinyu WANG, Xing XIE, Junfen XU, Weiguo LU",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B2300154"
}

%0 Journal Article
%T RAD51B-AS1 promotes the malignant biological behavior of ovarian cancer through upregulation of RAD51B
%A Xinyi WEI
%A Conghui WANG
%A Sangsang TANG
%A Qian YANG
%A Zhangjin SHEN
%A Jiawei ZHU
%A Xiaodong CHENG
%A Xinyu WANG
%A Xing XIE
%A Junfen XU
%A Weiguo LU
%J Journal of Zhejiang University SCIENCE B
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doi="https://doi.org/10.1631/jzus.B2300154"

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A1 - Conghui WANG
A1 - Sangsang TANG
A1 - Qian YANG
A1 - Zhangjin SHEN
A1 - Jiawei ZHU
A1 - Xiaodong CHENG
A1 - Xinyu WANG
A1 - Xing XIE
A1 - Junfen XU
A1 - Weiguo LU
J0 - Journal of Zhejiang University Science B
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PB - Zhejiang University Press & Springer
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doi="https://doi.org/10.1631/jzus.B2300154"


Abstract: 
Long non-coding RNAs (lncRNAs) play an indispensable role in the occurrence and development of ovarian cancer (OC). However, the potential involvement of lncRNAs in the progression of OC is largely unknown. To investigate the detailed roles and mechanisms of RAD51B-AS1, a novel lncRNA in OC, reverse transcription-quantitative polymerase chain reaction was performed to verify the expression of RAD51B-AS1. Cellular proliferation, metastasis, and apoptosis were detected using the Cell Counting Kit-8, colony-formation, Transwell, and flow cytometry assays, respectively. Mouse xenograft models were established for the detection of tumorigenesis. The results revealed RAD51B-AS1 was significantly upregulated in a highly metastatic human OC cell line and OC tissues. RAD51B-AS1 significantly increased the proliferation and metastasis of OC cells and enhanced their resistance to anoikis. Biogenetics prediction analysis revealed that the only target gene of RAD51B-AS1 was RAD51B. Subsequent gene function experiments revealed that RAD51B exerts the same biological effects as RAD51B-AS1. Rescue experiments demonstrated that the malignant biological behaviors promoted by RAD51B-AS1 overexpression were partially or completely reversed by RAD51B silencing in vitro and in vivo. Thus, RAD51B-AS1 promotes the malignant biological behavior of OC and activates the Akt/Bcl-2 signaling pathway, and these effects may be associated with the positive regulation of RAD51B expression. RAD51B-AS1 is expected to serve as a novel molecular biomarker for the diagnosis and prediction of poor prognosis in OC, and as a potential therapeutic target for disease management.

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