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On-line Access: 2024-08-27
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Xiaodan JIA, Ke LU, Xufang LIANG. Neuropeptide Y receptor Y8b (npy8br) regulates feeding and digestion in Japanese medaka (Oryzias latipes) larvae: evidence from gene knockout[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2300312 @article{title="Neuropeptide Y receptor Y8b (npy8br) regulates feeding and digestion in Japanese medaka (Oryzias latipes) larvae: evidence from gene knockout", %0 Journal Article TY - JOUR
神经肽受体Y8b(npy8br)调节日本青鳉(Oryzias latipes)仔鱼的摄食和消化:来自基因敲除的证据1华中农业大学水产学院,华中农业大学鳜鱼研究中心,中国武汉市,430070 2长江经济带大宗水生生物产业绿色发展教育部工程研究中心,中国武汉市,430070 摘要:神经肽Y受体Y8(NPY8R)是一种鱼类特异性受体,具有NPY8AR和NPY8BR两种亚型。生理过程中表达水平的变化以及心室注射后体内调节过程表明,NPY8BR在摄食调节中发挥着重要作用;目前只在少数鱼类中发现有这种作用。为了更好地了解NPY8BR的生理功能,特别是消化方面,我们利用CRISPR/Cas9技术构建了npy8br-/-日本青鳉(Oryzias latipes)。实验结果表明,npy8br基因缺失会影响青鳉仔鱼的摄食和消化能力,并最终影响其生长。具体来说,缺失npy8br会导致鳉鱼仔鱼摄食量减少和食欲相关基因(npy、agrp)表达水平降低。饲喂10 d(饲喂第10天)的npy8br-/-青鳉仔鱼在摄食8 h后消化道内仍有未完全消化的卤虫(Artemia nauplii),消化相关基因(amy、lpl、ctra和ctrb)mRNA表达量显著降低,且淀粉酶、胰蛋白酶和脂肪酶活性也显著降低。npy8br的缺失抑制了青鳉仔鱼的生长,显著降低了生长相关基因(gh和igf1)的表达。肠道组织切片显示,npy8br-/-青鳉仔鱼肠道受损,肠壁变薄,肠绒毛变短。本研究首次成功构建了鱼类的npy8br基因敲除模型,并证明npy8br在消化过程发挥着重要作用。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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