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Bio-Design and Manufacturing  2022 Vol.5 No.1 P.43-63

http://doi.org/10.1007/s42242-021-00154-3


Bioreactor design and validation for manufacturing strategies in tissue engineering


Author(s):  Diana Lim, Eric S. Renteria, Drake S. Sime, Young Min Ju, Ji Hyun Kim, Tracy Criswell, Thomas D. Shupe, Anthony Atala, Frank C. Marini, Metin N. Gurcan, Shay Soker, Joshua Hunsberger & James J. Yoo

Affiliation(s):  Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, NC, 27157, USA; more

Corresponding email(s):   jyoo@wakehealth.edu

Key Words:  Bioreactor, Monitoring sensors and automation, Manufacturing, Tissue engineering, Regenerative medicine


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Abstract: 
The fields of regenerative medicine and tissue engineering offer new therapeutic options to restore, maintain or improve tissue function following disease or injury. To maximize the biological function of a tissue-engineered clinical product, specific conditions must be maintained within a bioreactor to allow the maturation of the product in preparation for implantation. Specifically, the bioreactor should be designed to mimic the mechanical, electrochemical and biochemical environment that the product will be exposed to in vivo. Real-time monitoring of the functional capacity of tissue-engineered products during manufacturing is a critical component of the quality management process. The present review provides a brief overview of bioreactor engineering considerations. In addition, strategies for bioreactor automation, in-line product monitoring and quality assurance are discussed.

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