Full Text:   <3216>

Summary:  <10>

CLC number: 

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 0000-00-00

Cited: 0

Clicked: 2509

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Bio-Design and Manufacturing  2019 Vol.2 No.4 P.225-241

http://doi.org/10.1007/s42242-019-00050-x


Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering


Author(s):  João B. Costa, Joana Silva-Correia, Sandra Pina, Alain da Silva Morais, Sílvia Vieira, Hélder Pereira, João Espregueira-Mendes, Rui L. Reis, Joaquim M. Oliveira

Affiliation(s):  3B´s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and BiomimeticsUniversity of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineBarco GmrPortugal; more

Corresponding email(s):   miguel.oliveira@i3bs.uminho.pt

Key Words:  Patient-specific, Indirect printing, Hierarchical, Silk fibroin, Enzymatic-cross-linking, Meniscus


Share this article to: More

João B. Costa, Joana Silva-Correia, Sandra Pina, Alain da Silva Morais, Sílvia Vieira, Hélder Pereira, João Espregueira-Mendes, Rui L. Reis, Joaquim M. Oliveira. Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering[J]. Journal of Zhejiang University Science D, 2019, 2(4): 225-241.

@article{title="Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering",
author="João B. Costa, Joana Silva-Correia, Sandra Pina, Alain da Silva Morais, Sílvia Vieira, Hélder Pereira, João Espregueira-Mendes, Rui L. Reis, Joaquim M. Oliveira",
journal="Journal of Zhejiang University Science D",
volume="2",
number="4",
pages="225-241",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-019-00050-x"
}

%0 Journal Article
%T Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering
%A João B. Costa
%A Joana Silva-Correia
%A Sandra Pina
%A Alain da Silva Morais
%A Sílvia Vieira
%A Hélder Pereira
%A João Espregueira-Mendes
%A Rui L. Reis
%A Joaquim M. Oliveira
%J Journal of Zhejiang University SCIENCE D
%V 2
%N 4
%P 225-241
%@ 1869-1951
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-019-00050-x

TY - JOUR
T1 - Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering
A1 - João B. Costa
A1 - Joana Silva-Correia
A1 - Sandra Pina
A1 - Alain da Silva Morais
A1 - Sílvia Vieira
A1 - Hélder Pereira
A1 - João Espregueira-Mendes
A1 - Rui L. Reis
A1 - Joaquim M. Oliveira
J0 - Journal of Zhejiang University Science D
VL - 2
IS - 4
SP - 225
EP - 241
%@ 1869-1951
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-019-00050-x


Abstract: 
The complex meniscus tissue plays a critical role in the knee. The high susceptibility to injury has led to an intense pursuit for better tissue engineering regenerative strategies, where scaffolds play a major role. In this study, indirect printed hierarchical multilayered scaffolds composed by a silk fibroin (SF) upper layer and an 80/20 (w/w) ratio of SF/ionic-doped β-tricalcium phosphate (TCP) bottom layer were developed. Furthermore, a comparative analysis between two types of scaffolds produced using different SF concentrations, i.e., 8% (w/v) (Hi8) and 16% (w/v) (Hi16) was performed. In terms of architecture and morphology, the produced scaffolds presented homogeneous porosity in both layers and no differences were observed when comparing both scaffolds. A decrease in terms of mechanical performance of the scaffolds was observed when SF concentration decreased from 16 to 8% (w/v). Hi16 revealed a static compressive modulus of 0.66 ± 0.05 MPa and dynamical mechanical properties ranging from 2.17 ± 0.25 to 3.19 ± 0.38 MPa. By its turn, Hi8 presented a compressive modulus of 0.27 ± 0.08 MPa and dynamical mechanical properties ranging from 1.03 ± 0.08 MPa to 1.56 ± 0.13 MPa. In vitro bioactivity studies showed formation of apatite crystals onto the surface of Hi8 and Hi16 bottom layers. Human meniscus cells (hMCs) and human primary osteoblasts were cultured separately onto the top layer (SF8 and SF16) and bottom layer (SF8/TCP and SF16/TCP) of the hierarchical scaffolds Hi8 and Hi16, respectively. Both cell types showed good adhesion and proliferation as denoted by the live/dead staining, Alamar Blue assay and DNA quantification analysis. Subcutaneous implantation in mice revealed weak inflammation and scaffold’s integrity. The hierarchical indirect printed SF scaffolds can be promising candidate for meniscus TE scaffolding applications due their suitable mechanical properties, good biological performance and possibility of being applied in a patient-specific approach.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE