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CLC number: TS207.3

On-line Access: 2012-07-05

Received: 2011-12-31

Revision Accepted: 2012-02-29

Crosschecked: 2012-06-19

Cited: 12

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Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.7 P.545-554

http://doi.org/10.1631/jzus.B1200001


Development of a class-specific polyclonal antibody-based indirect competitive ELISA for detecting fluoroquinolone residues in milk


Author(s):  Guo-ying Fan, Ruo-song Yang, Jin-qing Jiang, Xin-yao Chang, Jun-jie Chen, Yong-hua Qi, Shi-xiu Wu, Xue-feng Yang

Affiliation(s):  College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; more

Corresponding email(s):   jjq5678@126.com

Key Words:  Norfloxacin, Fluoroquinolones, Indirect competitive ELISA, Class-specificity, Milk


Guo-ying Fan, Ruo-song Yang, Jin-qing Jiang, Xin-yao Chang, Jun-jie Chen, Yong-hua Qi, Shi-xiu Wu, Xue-feng Yang. Development of a class-specific polyclonal antibody-based indirect competitive ELISA for detecting fluoroquinolone residues in milk[J]. Journal of Zhejiang University Science B, 2012, 13(7): 545-554.

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author="Guo-ying Fan, Ruo-song Yang, Jin-qing Jiang, Xin-yao Chang, Jun-jie Chen, Yong-hua Qi, Shi-xiu Wu, Xue-feng Yang",
journal="Journal of Zhejiang University Science B",
volume="13",
number="7",
pages="545-554",
year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200001"
}

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%A Ruo-song Yang
%A Jin-qing Jiang
%A Xin-yao Chang
%A Jun-jie Chen
%A Yong-hua Qi
%A Shi-xiu Wu
%A Xue-feng Yang
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%P 545-554
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T1 - Development of a class-specific polyclonal antibody-based indirect competitive ELISA for detecting fluoroquinolone residues in milk
A1 - Guo-ying Fan
A1 - Ruo-song Yang
A1 - Jin-qing Jiang
A1 - Xin-yao Chang
A1 - Jun-jie Chen
A1 - Yong-hua Qi
A1 - Shi-xiu Wu
A1 - Xue-feng Yang
J0 - Journal of Zhejiang University Science B
VL - 13
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SP - 545
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1200001


Abstract: 
Modified 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) method was employed to synthesize the artificial antigen of norfloxacin (NOR), and New Zealand rabbits were used to produce anti-NOR polyclonal antibody (pAb). Based on the checkerboard titration, an indirect competitive enzyme-linked immunosorbent assay (icELISA) standard curve was established. This assay was sensitive and had a working range from 0.12 to 68.40 ng/ml, with the half maximal inhibitory concentration (IC50) and limit of detection (LOD) values of 2.7 ng/ml and 0.06 ng/ml, respectively. The produced pAb exhibited high cross-reactivity to fluoroquinolones (FQs) tested, and the IC50 values to enoxacin, ciprofloxacin, and pefloxacin were 3.1, 3.4, and 4.1 ng/ml, respectively. It also indicated that the concentrations of NaOH and methanol in assay buffer should not be higher than 10% and 30%. When spiked in milk at 5, 20, and 50 ng/ml, the recoveries for NOR, enoxacin, ciprofloxacin, and pefloxacin ranged 90.5%–98.0%, 84.0%–95.2%, 94.0%–106.0%, and 89.5%–100.0%, respectively. The results suggest that this class-specific pAb-based icELISA could be utilized for the primary screening of FQ residues in animal-original products.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Christodoulou, E.A., Samanidou, V.F., Papadoyannis, I.N., 2008. Development of an HPLC multi-residue method for the determination of ten quinolones in bovine liver and porcine kidney according to the European Union Decision 2002/657/EC. J. Sep. Sci., 31(1):119-127.

[2]Delepine, B., Hurtaud-Pessel, D., Sanders, P., 1998. Simultaneous determination of six quinolones in pig muscle by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. Analyst, 123(12):2743-2747.

[3]Dufresne, G., Fouquet, A., Forsyth, D., Tittlemier, S.A., 2007. Multiresidue determination of quinolone and fluoroquinolone antibiotics in fish and shrimp by liquid chromatography/tandem mass spectrometry. J. AOAC Int., 90(2):604-612.

[4]Hassouan, M.K., Ballesteros, O., Zafra, A., Vílchez, J.L., Navalón, A., 2007. Multiresidue method for simultaneous determination of quinolone antibacterials in pig kidney samples by liquid chromatography with fluorescence detection. J. Chromatogr. B, 859(2):282-288.

[5]Hermo, M.P., Nemutlu, E., Kir, S., Barrón, D., Barbosa, J., 2008. Improved determination of quinolones in milk at their MRL levels using LC-UV, LC-FD, LC-MS and LC-MS/MS and validation in line with regulation 2002/657/EC. Anal. Chim. Acta, 613(1):98-107.

[6]Huang, B., Yin, Y., Lu, L., Ding, H., Wang, L., Yu, T., Zhu, J.J., Zheng, X.D., Zhang, Y.Z., 2010. Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 11(10):812-818.

[7]Huang, H.G., Jiang, J.Q., Zhang, H.T., Wang, Z.L., Li, X.P., 2011. Development of a Polyclonal Antibody Based Immunoassay for Detection of Molybdenum Levels in Environmental Water. In: International Conference on Awareness Science and Technology (iCAST), 27–30 Sept. 2011. Dalian, China, p.103-106.

[8]Huet, A.C., Charlier, C., Tittlemier, S.A., Singh, G., Benrejeb, S., Delahaut, P., 2006. Simultaneous determination of (fluoro)quinolone antibiotics in kidney, marine products, eggs, and muscle by enzyme-linked immunosorbent assay (ELISA). J. Agric. Food Chem., 54(8):2822-2827.

[9]Jiang, J.Q., Wang, Z.L., Zhang, H.T., Zhang, X.J., Liu, X.Y., Wang, S.H., 2011a. Monoclonal antibody-based ELISA and colloidal gold immunoassay for detecting 19-nortestosterone residue in animal tissues. J. Agric. Food Chem., 59(18):9763-9769.

[10]Jiang, J.Q., Zhang, H.T., Li, G.L., Wang, Z.L., Wang, J.H., Zhao, H.Z., 2011b. Preparation of anti-nortestosterone antibodies and development of an indirect heterologous competitive enzyme-linked immunosorbent assay to detect nortestosterone residues in animal urines. Anal. Lett., 44(14):2373-2393.

[11]Liu, Y., Zhang, C.Z., Yu, X.Y., Zhang, Z.Y., Zhang, X., Liu, R.R., Liu, X.J., Gong, Z.M., 2007. Development and evaluation of immunoassay for zeranol in bovine urine. J. Zhejiang Univ.-Sci. B, 8(12):900-905.

[12]Lu, S., Zhang, Y., Liu, J., Zhao, C., Liu, W., Xi, R., 2006. Preparation of anti-pefloxacin antibody and development of an indirect competitive enzyme-linked immunosorbent assay for detection of pefloxacin residue in chicken liver. J. Agric. Food Chem., 54(19):6995-7000.

[13]San Martín, B., Cornejo, J., Iragüen, D., Hidalgo, H., Anadón, A., 2007. Depletion study of enrofloxacin and its metabolite ciprofloxacin in edible tissues and feathers of white leghorn hens by liquid chromatography coupled with tandem mass spectrometry. J. Food Prot., 70(8):1952-1957.

[14]Sheng, W., Xu, T., Ma, H., Wang, X., Li, Q.X., Li, J., 2009. Development of an indirect competitive enzyme-linked immunosorbent assay for detection of danofloxacin residues in beef, chicken and pork meats. Food Agric. Immunol., 20(1):35-47.

[15]Wang, Z., Zhu, Y., Ding, S., He, F., Beier, R.C., Li, J., Jiang, H., Feng, C., Wan, Y., Zhang, S., et al., 2007. Development of a monoclonal antibody-based broad-specificity ELISA for fluoroquinolone antibiotics in foods and molecular modeling studies of cross-reactive compounds. Anal. Chem., 79(12):4471-4483.

[16]Wu, J.X., Zhang, S.E., Zhou, X.P., 2010. Monoclonal antibody-based ELISA and colloidal gold-based immunochromatographic assay for streptomycin residue detection in milk and swine urine. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 11(1):52-60.

[17]Zhang, H.T., Jiang, J.Q., Wang, Z.L., Chang, X.Y., Liu, X.Y., Wang, S.H., Zhao, K., Chen, J.S., 2011. Development of an indirect competitive ELISA for simultaneous detection of enrofloxacin and ciprofloxacin. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 12(11):884-891.

[18]Zhang, L., Jiang, J.Q., Li, G.L., Wang, Z.L., Yang, X.F., Ma, J.Y., Zhang, H.T., Huang, H.G., Liu, X.Y., 2011. Development and optimization of a monoclonal antibody based indirect competitive ELISA for detecting fluoroquinolone residue in milk. J. Food Agric. Environ., 9(2):113-120.

[19]Zhu, Y., Li, L., Wang, Z., Chen, Y., Zhao, Z., Zhu, L., Wu, X., Wan, Y., He, F., Shen, J., 2008. Development of an immunochromatography strip for the rapid detection of 12 fluoroquinolones in chicken muscle and liver. J. Agric. Food Chem., 56(14):5469-5474.

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