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CLC number: TS207.3

On-line Access: 2010-10-05

Received: 2010-02-21

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.10 P.812-818

10.1631/jzus.B1000055


Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk


Author(s):  Bin Huang, Yun Yin, Lei Lu, Hai Ding, Lin Wang, Ting Yu, Jia-jin Zhu, Xiao-dong Zheng, Yan-zhen Zhang

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   zyz85@hotmail.com

Key Words:  Ciprofloxacin, Competitive enzyme-linked immunosorbent assay (ELISA), Rabbit monoclonal antibody, Residues, Cross-reactivity


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Bin Huang, Yun Yin, Lei Lu, Hai Ding, Lin Wang, Ting Yu, Jia-jin Zhu, Xiao-dong Zheng, Yan-zhen Zhang. Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk[J]. Journal of Zhejiang University Science B, 2010, 11(10): 812-818.

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author="Bin Huang, Yun Yin, Lei Lu, Hai Ding, Lin Wang, Ting Yu, Jia-jin Zhu, Xiao-dong Zheng, Yan-zhen Zhang",
journal="Journal of Zhejiang University Science B",
volume="11",
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pages="812-818",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000055"
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%T Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk
%A Bin Huang
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%A Lei Lu
%A Hai Ding
%A Lin Wang
%A Ting Yu
%A Jia-jin Zhu
%A Xiao-dong Zheng
%A Yan-zhen Zhang
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%P 812-818
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%I Zhejiang University Press & Springer
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T1 - Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk
A1 - Bin Huang
A1 - Yun Yin
A1 - Lei Lu
A1 - Hai Ding
A1 - Lin Wang
A1 - Ting Yu
A1 - Jia-jin Zhu
A1 - Xiao-dong Zheng
A1 - Yan-zhen Zhang
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 10
SP - 812
EP - 818
%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1000055


Abstract: 
A convenient competitive enzyme-linked immunosorbent assay (ELISA) for ciprofloxacin (CPFX) was developed by using rabbit monoclonal antibodies (RabMAbs) against a hapten-protein conjugate of CPFX-bovine serum albumin (BSA). The indirect competitive ELISA of CPFX had a concentration at 50% inhibition (IC50) of 1.47 ng/ml and a limit of detection (LOD) of 0.095 ng/ml. The mAb exhibited some cross-reactivity, however, not so high with enrofloxacin (28.8%), ofloxacin (13.1%), norfloxacin (11.0%), fleroxacin (22.6%), and pefloxacin (20.4%). And it showed almost no cross-reactivity with other antibiotics or sulfonamides evaluated in this study. The competitive ELISA kit developed here could be used as a screening tool to detect and control illegal addition of CPFX in food products. This kit had been applied to milk detection and the recovery rates from samples spiked by CPFX were in a range of 63.02%–84.60%, with coefficients of variation of less than 12.2%.

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