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CLC number: Q74

On-line Access: 2016-04-05

Received: 2015-11-11

Revision Accepted: 2015-12-28

Crosschecked: 2016-03-13

Cited: 5

Clicked: 1284

Citations:  Bibtex RefMan EndNote GB/T7714


Yi-rong Guo


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.4 P.282-293


Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds

Author(s):  Rui Liu, Ying Liu, Mei-jing Lan, Niusha Taheri, Jing-li Cheng, Yi-rong Guo, Guo-nian Zhu

Affiliation(s):  Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   yirongguo@zju.edu.cn

Key Words:  Water-soluble adjuvant, Monoclonal antibody, Hapten-specific antibody, High affinity

Rui Liu, Ying Liu, Mei-jing Lan, Niusha Taheri, Jing-li Cheng, Yi-rong Guo, Guo-nian Zhu. Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds[J]. Journal of Zhejiang University Science B, 2016, 17(4): 282-293.

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author="Rui Liu, Ying Liu, Mei-jing Lan, Niusha Taheri, Jing-li Cheng, Yi-rong Guo, Guo-nian Zhu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds
%A Rui Liu
%A Ying Liu
%A Mei-jing Lan
%A Niusha Taheri
%A Jing-li Cheng
%A Yi-rong Guo
%A Guo-nian Zhu
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 4
%P 282-293
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500278

T1 - Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds
A1 - Rui Liu
A1 - Ying Liu
A1 - Mei-jing Lan
A1 - Niusha Taheri
A1 - Jing-li Cheng
A1 - Yi-rong Guo
A1 - Guo-nian Zhu
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 4
SP - 282
EP - 293
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500278

A water-soluble adjuvant named QuickAntibody (QA) was introduced into the procedure of mouse immunization for the development of hapten-specific monoclonal antibodies (mAbs), using four kinds of pesticides as model compounds. Compared with conventional Freund’s adjuvants, QA treatments offered relatively low but acceptable antiserum titers after three inoculations, gave little adverse effects to the experimental animals, and were preferable in harvesting splenocytes during the steps of cell fusion. Afterwards, hybridomas from the QA group were prepared and screened by both non-competitive and competitive indirect enzyme-linked immunosorbent assays (ELISAs). The efficiency of gaining immune-positive hybridomas was satisfactory, and the resultant mAbs showed sensitivities (half maximal inhibitory concentration (IC50)) of 0.91, 2.46, 3.72, and 6.22 ng/ml to triazophos, parathion, chlorpyrifos, and fenpropathrin, respectively. Additionally, the performance of QA adjuvant was further confirmed by acquiring a high-affinity mAb against okadaic acid (IC50 of 0.36 ng/ml) after three immunizations. These newly developed mAbs showed similar or even better sensitivities compared with previously reported mAbs specific to the corresponding analytes. This study suggested that the easy-to-use adjuvant could be applicable to the efficient generation of highly sensitive mAbs against small compounds.


方法:本研究以4种农药和1种海洋毒素为目标分析物,采用一种新型水溶性佐剂与小分子抗原相互 混合,直接对Balb/c小鼠进行小腿肌肉注射免疫。 经过2~3次免疫后进行腹腔注射常规末次免疫,细胞 融合后筛选出了相应的杂交瘤细胞株,制备并鉴定了针对上述半抗原小分子的特异性单抗。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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