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CLC number: S432
On-line Access: 2017-12-05
Received: 2016-12-09
Revision Accepted: 2017-04-17
Crosschecked: 2017-11-22
Cited: 0
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Citations: Bibtex RefMan EndNote GB/T7714
Monoclonal antibody-based serological assays for detection of Potato virus S in potato plants
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Ge Song, Jia-yu Wu, Yan Xie, Yong Liu, Ya-juan Qian, Xue-ping Zhou, Jian-xiang Wu. Monoclonal antibody-based serological assays for detection of Potato virus S in potato plants[J]. Journal of Zhejiang University Science B, 2017, 18(12): 1075-1082.
@article{title="Monoclonal antibody-based serological assays for detection of Potato virus S in potato plants",
author="Ge Song, Jia-yu Wu, Yan Xie, Yong Liu, Ya-juan Qian, Xue-ping Zhou, Jian-xiang Wu",
journal="Journal of Zhejiang University Science B",
volume="18",
number="12",
pages="1075-1082",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600561"
}
%0 Journal Article
%T Monoclonal antibody-based serological assays for detection of Potato virus S in potato plants
%A Ge Song
%A Jia-yu Wu
%A Yan Xie
%A Yong Liu
%A Ya-juan Qian
%A Xue-ping Zhou
%A Jian-xiang Wu
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 12
%P 1075-1082
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600561
TY - JOUR
T1 - Monoclonal antibody-based serological assays for detection of Potato virus S in potato plants
A1 - Ge Song
A1 - Jia-yu Wu
A1 - Yan Xie
A1 - Yong Liu
A1 - Ya-juan Qian
A1 - Xue-ping Zhou
A1 - Jian-xiang Wu
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 12
SP - 1075
EP - 1082
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600561
Abstract: potato virus S (PVS) often causes significant losses in potato production in potato-growing countries. In this study, the ordinary strain of PVS (PVSO) was purified from PVS-infected potato plants and used as the immunogen to produce hybridomas secreting monoclonal antibodies (MAbs). Five highly specific and sensitive murine MAbs (1A3, 16C10, 18A9, 20B12, and 22H4) against PVS were prepared using conventional hybridoma technology. Using these MAbs, tissue print-enzyme-linked immunosorbent assay (ELISA), dot-ELISA, and double-antibody sandwich (DAS)-ELISA were developed for sensitive and specific detection of PVS infection in potato plants. The results of sensitivity assays revealed that PVS could be reliably detected in PVS-infected leaf crude extracts diluted at 1:10 240 and 1:163 840 (w/v, g/ml) in phosphate buffer saline (PBS) by dot-ELISA and DAS-ELISA, respectively. Twenty-two samples collected from potato fields in Yunnan Province, China were tested for PVS infection using the serological assays we had developed, and 14 of them were found to be positive. This indicates that PVS is now prevalent in potato fields in Yunnan Province.
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