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Received: 2012-10-10

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.7 P.555-562

10.1631/jzus.B1200275


Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China


Author(s):  Jian-xiang Wu, Qiang Wang, Huan Liu, Ya-juan Qian, Yan Xie, Xue-ping Zhou

Affiliation(s):  State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   xiey@zju.edu.cn, zzhou@zju.edu.cn

Key Words:  Maize chlorotic mottle virus (MCMV), Immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR), Triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), Monoclonal antibody (MAb), Dot-immunobinding assay (DIBA)


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Jian-xiang Wu, Qiang Wang, Huan Liu, Ya-juan Qian, Yan Xie, Xue-ping Zhou. Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China[J]. Journal of Zhejiang University Science B, 2013, 14(7): 555-562.

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author="Jian-xiang Wu, Qiang Wang, Huan Liu, Ya-juan Qian, Yan Xie, Xue-ping Zhou",
journal="Journal of Zhejiang University Science B",
volume="14",
number="7",
pages="555-562",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200275"
}

%0 Journal Article
%T Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China
%A Jian-xiang Wu
%A Qiang Wang
%A Huan Liu
%A Ya-juan Qian
%A Yan Xie
%A Xue-ping Zhou
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 7
%P 555-562
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200275

TY - JOUR
T1 - Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China
A1 - Jian-xiang Wu
A1 - Qiang Wang
A1 - Huan Liu
A1 - Ya-juan Qian
A1 - Yan Xie
A1 - Xue-ping Zhou
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 7
SP - 555
EP - 562
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200275


Abstract: 
maize chlorotic mottle virus (MCMV) infects maize plants and causes significant losses in corn production worldwide. In this study, purified MCMV particles were used as the immunogen to produce monoclonal antibodies (MAbs) and polyclonal antibodies (PAbs). Four murine MAbs (4B8, 8C11, 6F4, and 9G1) against MCMV were obtained through the hybridoma technology. The triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), dot-immunobinding assay (DIBA), and immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) using the MAb 4B8 were then developed for sensitive, specific, and rapid detection of MCMV in fields. MCMV could be detected in infected leaf crude extracts at dilutions of 1:327680, 1:64000, and 1:3276800 (w/v, g/ml) by TAS-ELISA, DIBA, and IC-RT-PCR, respectively. One hundred and sixty-one maize field samples showing virus-like symptoms and sixty-nine symptomless maize field samples from ten different provinces of China were collected and screened for the presence of MCMV using the established serological methods. A phylogenetic tree was constructed based on the full length CP genes and Chinese MCMV isolates formed one branch with Thailand isolates. The detection results demonstrated that MCMV is one of most prevalent viruses infecting maize in the Yunnan and Sichuan provinces of China.

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