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On-line Access: 2017-12-05

Received: 2016-12-19

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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.12 P.1064-1074


Polymorphisms of the IGF1 gene and their association with growth traits, serum concentration and expression rate of IGF1 and IGF1R in buffalo

Author(s):  Mohammed A. El-Magd, Ayman A. Saleh, Abeer A. Nafeaa, Shymaa M. El-Komy, Mohamed A. Afifi

Affiliation(s):  Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, El-Geish Street, Kafrelsheikh, Egypt; more

Corresponding email(s):   mohrizk73@yahoo.com

Key Words:  Insulin-like growth factor 1 (IGF1), IGF1R, Single nucleotide polymorphism (SNP), Growth traits, Buffalo

Mohammed A. El-Magd, Ayman A. Saleh, Abeer A. Nafeaa, Shymaa M. El-Komy, Mohamed A. Afifi. Polymorphisms of the IGF1 gene and their association with growth traits, serum concentration and expression rate of IGF1 and IGF1R in buffalo[J]. Journal of Zhejiang University Science B, 2017, 18(12): 1064-1074.

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author="Mohammed A. El-Magd, Ayman A. Saleh, Abeer A. Nafeaa, Shymaa M. El-Komy, Mohamed A. Afifi",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Polymorphisms of the IGF1 gene and their association with growth traits, serum concentration and expression rate of IGF1 and IGF1R in buffalo
%A Mohammed A. El-Magd
%A Ayman A. Saleh
%A Abeer A. Nafeaa
%A Shymaa M. El-Komy
%A Mohamed A. Afifi
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 12
%P 1064-1074
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600573

T1 - Polymorphisms of the IGF1 gene and their association with growth traits, serum concentration and expression rate of IGF1 and IGF1R in buffalo
A1 - Mohammed A. El-Magd
A1 - Ayman A. Saleh
A1 - Abeer A. Nafeaa
A1 - Shymaa M. El-Komy
A1 - Mohamed A. Afifi
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 12
SP - 1064
EP - 1074
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600573

The insulin-like growth factor 1 (IGF1) gene is a member of the group of somatotropin axis genes that play a significant role in cell proliferation and growth of muscles. Here, we searched for polymorphisms in buffalo IGF1 and found two novel single nucleotide polymorphisms (SNPs), G64A and G280A, in the noncoding sequences of exon 1 and exon 4, respectively. Statistical analysis of different genotypes showed that the individuals with GG genotypes had significantly (P<0.05) higher body weight (BW) and average daily gain (ADG) than those with other genotypes at ages of 3–6 months in G64A SNP and 6–9 months in G280A SNP. The combined genotypes of these two SNPs produced three haplotypes, GG/GG, AG/AG, and AA/AA, which were significantly associated (P<0.0001) with BW and ADG at an age from 3 to 12 months. buffaloes with the homozygous GG/GG haplotype showed higher growth performance than other buffaloes. The two SNPs were correlated with mRNA levels of IGF1 and IGF1 receptor (IGF1R) in semitendinosus muscle as well as with the serum concentration level of IGF1. Also, buffaloes with GG/GG haplotype showed higher mRNA and serum concentration levels. The data revealed that these two SNPs could be valuable genetic markers for selection of Egyptian buffaloes for better performance in the population.


目的:探讨胰岛素样生长因子1(insulin-like growth factor 1,IGF1)基因单核苷酸多态性(SNP)与埃及水牛生长性状的关联性。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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