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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.12 P.1064-1074

http://doi.org/10.1631/jzus.B1600573


Polymorphisms of the IGF1 gene and their association with growth traits, serum concentration and expression rate of IGF1 and IGF1R in buffalo


Author(s):  Mohammed A. El-Magd, Ayman A. Saleh, Abeer A. Nafeaa, Shymaa M. El-Komy, Mohamed A. Afifi

Affiliation(s):  Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, El-Geish Street, Kafrelsheikh, Egypt; more

Corresponding email(s):   mohrizk73@yahoo.com

Key Words:  Insulin-like growth factor 1 (IGF1), IGF1R, Single nucleotide polymorphism (SNP), Growth traits, Buffalo


Mohammed A. El-Magd, Ayman A. Saleh, Abeer A. Nafeaa, Shymaa M. El-Komy, Mohamed A. Afifi. Polymorphisms of the IGF1 gene and their association with growth traits, serum concentration and expression rate of IGF1 and IGF1R in buffalo[J]. Journal of Zhejiang University Science B, 2017, 18(12): 1064-1074.

@article{title="Polymorphisms of the IGF1 gene and their association with growth traits, serum concentration and expression rate of IGF1 and IGF1R in buffalo",
author="Mohammed A. El-Magd, Ayman A. Saleh, Abeer A. Nafeaa, Shymaa M. El-Komy, Mohamed A. Afifi",
journal="Journal of Zhejiang University Science B",
volume="18",
number="12",
pages="1064-1074",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600573"
}

%0 Journal Article
%T Polymorphisms of the IGF1 gene and their association with growth traits, serum concentration and expression rate of IGF1 and IGF1R in buffalo
%A Mohammed A. El-Magd
%A Ayman A. Saleh
%A Abeer A. Nafeaa
%A Shymaa M. El-Komy
%A Mohamed A. Afifi
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 12
%P 1064-1074
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600573

TY - JOUR
T1 - Polymorphisms of the IGF1 gene and their association with growth traits, serum concentration and expression rate of IGF1 and IGF1R in buffalo
A1 - Mohammed A. El-Magd
A1 - Ayman A. Saleh
A1 - Abeer A. Nafeaa
A1 - Shymaa M. El-Komy
A1 - Mohamed A. Afifi
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 12
SP - 1064
EP - 1074
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1600573


Abstract: 
The insulin-like growth factor 1 (IGF1) gene is a member of the group of somatotropin axis genes that play a significant role in cell proliferation and growth of muscles. Here, we searched for polymorphisms in buffalo IGF1 and found two novel single nucleotide polymorphisms (SNPs), G64A and G280A, in the noncoding sequences of exon 1 and exon 4, respectively. Statistical analysis of different genotypes showed that the individuals with GG genotypes had significantly (P<0.05) higher body weight (BW) and average daily gain (ADG) than those with other genotypes at ages of 3–6 months in G64A SNP and 6–9 months in G280A SNP. The combined genotypes of these two SNPs produced three haplotypes, GG/GG, AG/AG, and AA/AA, which were significantly associated (P<0.0001) with BW and ADG at an age from 3 to 12 months. buffaloes with the homozygous GG/GG haplotype showed higher growth performance than other buffaloes. The two SNPs were correlated with mRNA levels of IGF1 and IGF1 receptor (IGF1R) in semitendinosus muscle as well as with the serum concentration level of IGF1. Also, buffaloes with GG/GG haplotype showed higher mRNA and serum concentration levels. The data revealed that these two SNPs could be valuable genetic markers for selection of Egyptian buffaloes for better performance in the population.

水牛IGF1基因多态性与其生长性状、血清浓度以及IGF1和IGF1R基因表达的相关性研究

目的:探讨胰岛素样生长因子1(insulin-like growth factor 1,IGF1)基因单核苷酸多态性(SNP)与埃及水牛生长性状的关联性。
创新点:发现IGF1基因多态性对埃及水牛的生长性状、血液生化指标和基因表达有显著影响,并为埃及水牛的选育提供重要的分子遗传标记。
方法:以200头埃及水牛为试验对象,对其IGF1基因的多态性进行检测,并分析其与生长性状、血液生理生化指标和基因表达的相关性。
结论:在水牛IGF1基因多态性分析中,发现两个新的SNP位点(G64A和G280A)分别分布在外显子1和外显子4的非编码区。不同基因型的统计分析表明,G64A和G280A位点的GG基因型水牛个体分别在3~6月龄和6~9月龄的体重(BW)和平均日增重(ADG)均显著高于其他基因型(P<0.05)。这两个SNPs位点的组合基因型产生了三种单倍体GG/GG、AG/AG和AA/AA。在3~12月龄的水牛个体中,单倍体基因型与BW和ADG存在显著关联(P<0.0001)。纯合的GG/GG单倍体基因型水牛生长性能优于其他水牛。两个SNP位点与半腱肌中IGF1IGF1R的mRNA水平以及IGF1血清浓度水平相关。此外,GG/GG单倍体水牛表现出较高的mRNA和血清浓度水平。综上所述,这两种SNP位点G64A和G280A可作为埃及水牛生长性状选育的重要分子遗传标记。

关键词:胰岛素样生长因子1(IGF1);胰岛素样生长因子1受体(IGF1R);单核苷酸多态性(SNP);生长性状;水牛

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