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CLC number: R917; O657.1

On-line Access: 2012-01-18

Received: 2011-03-10

Revision Accepted: 2011-07-26

Crosschecked: 2011-12-10

Cited: 2

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.2 P.118-125


Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine

Author(s):  Jian Yi, Meng Meng, Zhong-qiu Liu, Jin-fang Zhi, Yuan-yang Zhang, Jing Xu, Ya-bin Wang, Jin-ting Liu, Ri-mo Xi

Affiliation(s):  School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China; more

Corresponding email(s):   jintliu@sdu.edu.cn, xirimo2000@yahoo.com

Key Words:  Gatifloxacin, Drug residue, Antibody, Carbon fiber electrode, Electrochemical immunoassay

Jian Yi, Meng Meng, Zhong-qiu Liu, Jin-fang Zhi, Yuan-yang Zhang, Jing Xu, Ya-bin Wang, Jin-ting Liu, Ri-mo Xi. Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine[J]. Journal of Zhejiang University Science B, 2012, 13(2): 118-125.

@article{title="Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine",
author="Jian Yi, Meng Meng, Zhong-qiu Liu, Jin-fang Zhi, Yuan-yang Zhang, Jing Xu, Ya-bin Wang, Jin-ting Liu, Ri-mo Xi",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine
%A Jian Yi
%A Meng Meng
%A Zhong-qiu Liu
%A Jin-fang Zhi
%A Yuan-yang Zhang
%A Jing Xu
%A Ya-bin Wang
%A Jin-ting Liu
%A Ri-mo Xi
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 2
%P 118-125
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100073

T1 - Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine
A1 - Jian Yi
A1 - Meng Meng
A1 - Zhong-qiu Liu
A1 - Jin-fang Zhi
A1 - Yuan-yang Zhang
A1 - Jing Xu
A1 - Ya-bin Wang
A1 - Jin-ting Liu
A1 - Ri-mo Xi
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 2
SP - 118
EP - 125
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100073

To detect gatifloxacin (GAT) residue in swine urine, an electrochemical immunoassay was established. An indirect competitive immunoassay was developed, in which the coating antigen is immobilized in an enzyme-linked immunosorbent assay (ELISA) plate and GAT residue from the sample competes with the limited binding sites in added anti-GAT antibody. Horseradish peroxidase (HRP) conjugated to goat anti-rabbit IgG was used as the enzymatic label. A carbon fiber working electrode was constructed and current signals were detected by using hydrogen peroxide as a substrate and hydroquinone as an electrochemical mediator. The electrochemical immunoassay was evaluated by analysis of GAT in buffer or swine urine and an average value of half inhibition concentration (IC50) of 8.9 ng/ml was obtained. Excellent specificity of the antibody was achieved with little cross-reaction with lomefloxacin (3.0%), ciprofloxacin (3.0%), and ofloxacin (1.9%) among commonly used (fluoro)quinolones. In conclusion, the immunoassay system developed in this research can be used as a rapid, powerful and on-site analytical tool to detect GAT residue in foods and food products.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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