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CLC number: Q812

On-line Access: 2012-01-18

Received: 2011-04-20

Revision Accepted: 2011-07-27

Crosschecked: 2012-01-03

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.2 P.111-117

10.1631/jzus.B1100131


Immobilization of foreign protein into polyhedra of Bombyx mori nucleopolyhedrovirus (BmNPV)


Author(s):  Xing-wei Xiang, Rui Yang, Lin Chen, Xiao-long Hu, Shao-fang Yu, Cui-ping Cao, Xiao-feng Wu

Affiliation(s):  College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   wuxiaofeng@zju.edu.cn

Key Words:  Bombyx mori nucleopolyhedrovirus, Polyhedra, Foreign protein, Protein immobilization


Xing-wei Xiang, Rui Yang, Lin Chen, Xiao-long Hu, Shao-fang Yu, Cui-ping Cao, Xiao-feng Wu. Immobilization of foreign protein into polyhedra of Bombyx mori nucleopolyhedrovirus (BmNPV)[J]. Journal of Zhejiang University Science B, 2012, 13(2): 111-117.

@article{title="Immobilization of foreign protein into polyhedra of Bombyx mori nucleopolyhedrovirus (BmNPV)",
author="Xing-wei Xiang, Rui Yang, Lin Chen, Xiao-long Hu, Shao-fang Yu, Cui-ping Cao, Xiao-feng Wu",
journal="Journal of Zhejiang University Science B",
volume="13",
number="2",
pages="111-117",
year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100131"
}

%0 Journal Article
%T Immobilization of foreign protein into polyhedra of Bombyx mori nucleopolyhedrovirus (BmNPV)
%A Xing-wei Xiang
%A Rui Yang
%A Lin Chen
%A Xiao-long Hu
%A Shao-fang Yu
%A Cui-ping Cao
%A Xiao-feng Wu
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 2
%P 111-117
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100131

TY - JOUR
T1 - Immobilization of foreign protein into polyhedra of Bombyx mori nucleopolyhedrovirus (BmNPV)
A1 - Xing-wei Xiang
A1 - Rui Yang
A1 - Lin Chen
A1 - Xiao-long Hu
A1 - Shao-fang Yu
A1 - Cui-ping Cao
A1 - Xiao-feng Wu
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 2
SP - 111
EP - 117
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100131


Abstract: 
In the late phase of Bombyx mori nucleopolyhedrovirus (BmNPV) infection, a large amount of polyhedra appear in the infected cell nucleolus, these polyhedra being dense protein crystals protecting the incorporated virions from the harsh environment. To investigate whether the foreign protein could be immobilized into the polyhedra of BmNPV, two recombinant baculoviruses were generated by a novel BmNPV polyhedrin-plus (polh+) Bac-to-Bac system, designated as vBmBac(polh+)-enhanced green fluorescent protein (EGFP) and vBmBac(polh+)-LacZ, which can express the polyhedrin and foreign protein simultaneously. Light microscopy analysis showed that all viruses produced polyhedra of normal appearance. Green fluorescence can be apparently detected on the surface of the vBmBac(polh+)-EGFP polyhedra, but not the BmNPV polyhedra. Fluorescence analysis and anti-desiccation testing confirmed that EGFP was embedded in the polyhedra. As expected, the vBmBac(polh+)-LacZ polyhedra contained an amount of LacZ and had a higher β-galactosidase activity. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting were also performed to verify if the foreign proteins were immobilized into polyhedra. This study provides a new inspiration for efficient preservation of useful proteins and development of new pesticides with toxic proteins.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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