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CLC number: S821.5

On-line Access: 2016-10-02

Received: 2016-05-19

Revision Accepted: 2016-08-04

Crosschecked: 2016-09-10

Cited: 1

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Zhi-mei Tian

http://orcid.org/0000-0002-1738-7454

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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.10 P.742-751

http://doi.org/10.1631/jzus.B1600229


Influence of low protein diets on gene expression of digestive enzymes and hormone secretion in the gastrointestinal tract of young weaned piglets


Author(s):  Zhi-mei Tian, Xian-yong Ma, Xue-fen Yang, Qiu-li Fan, Yun-xia Xiong, Yue-qin Qiu, Li Wang, Xiao-lu Wen, Zong-yong Jiang

Affiliation(s):  State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Corresponding email(s):   407986619@qq.com, jiangz28@qq.com

Key Words:  Dietary protein, Piglet, Gastrointestinal tract, Pancreas, Digestion, Hormone


Zhi-mei Tian, Xian-yong Ma, Xue-fen Yang, Qiu-li Fan, Yun-xia Xiong, Yue-qin Qiu, Li Wang, Xiao-lu Wen, Zong-yong Jiang. Influence of low protein diets on gene expression of digestive enzymes and hormone secretion in the gastrointestinal tract of young weaned piglets[J]. Journal of Zhejiang University Science B, 2016, 17(10): 742-751.

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author="Zhi-mei Tian, Xian-yong Ma, Xue-fen Yang, Qiu-li Fan, Yun-xia Xiong, Yue-qin Qiu, Li Wang, Xiao-lu Wen, Zong-yong Jiang",
journal="Journal of Zhejiang University Science B",
volume="17",
number="10",
pages="742-751",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600229"
}

%0 Journal Article
%T Influence of low protein diets on gene expression of digestive enzymes and hormone secretion in the gastrointestinal tract of young weaned piglets
%A Zhi-mei Tian
%A Xian-yong Ma
%A Xue-fen Yang
%A Qiu-li Fan
%A Yun-xia Xiong
%A Yue-qin Qiu
%A Li Wang
%A Xiao-lu Wen
%A Zong-yong Jiang
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 10
%P 742-751
%@ 1673-1581
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600229

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T1 - Influence of low protein diets on gene expression of digestive enzymes and hormone secretion in the gastrointestinal tract of young weaned piglets
A1 - Zhi-mei Tian
A1 - Xian-yong Ma
A1 - Xue-fen Yang
A1 - Qiu-li Fan
A1 - Yun-xia Xiong
A1 - Yue-qin Qiu
A1 - Li Wang
A1 - Xiao-lu Wen
A1 - Zong-yong Jiang
J0 - Journal of Zhejiang University Science B
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SP - 742
EP - 751
%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1600229


Abstract: 
To investigate dietary protein level effects on digestive mechanisms, weaned piglets were fed for 45 d with diets containing 20%, 17%, or 14% crude protein (CP) supplemented to meet requirements for essential amino acids. This article describes the influence of dietary protein on gastrointestinal hormones and expression of an array of digestive enzymes in the gastrointestinal tract and pancreas. Results indicated that there were no significant differences in expression of enzymes involved in carbohydrate digestion, except for maltase in the duodenum. In the jejunum, amylase expression in pigs fed 20% CP was much higher than that in pigs fed other diets (P<0.05) and maltase expression in those fed 17% CP was higher than that in other treatments (P<0.05). Although there were no remarkable differences in expression of aminopeptidase in the small intestine or carboxypeptidase in the pancreas (P>0.05), there was a trend towards higher expression of various proteases in pigs fed 17% CP. The duodenal expression of enteropeptidase in diets with 14% and 17% CP was significantly higher than that with 20% CP (P<0.05), but treatment differences did not existed in jejunum (P>0.05). The expression of GPR93 as a nutrient-responsive G protein-coupled receptor in 14% and 17% CP diets was significantly higher than that in 20% CP diet in the small intestine (P<0.05). The expressions of genes for pancreatic enzymes, lipase and elastase, were significantly higher in pigs fed diets with low CP, while similar trends occurred for carboxypeptidase, chymotrypsin and amylase. Conversely, the gastric expressions of pepsinogen A and progastricsin were lower with the 17% CP diet. Differences between treatments were found in the gastric antral contents of cholecystokinin and somatostatin: both increased in pigs fed 17% CP, accompanied by decreased content of motilin, which was also seen in plasma concentrations. These patterns were not reflected in duodenal contents. In general, 17% dietary CP was beneficial to the digestion of nutrient substance in the gastrointestinal tract.

低蛋白日粮对断奶仔猪胃肠道消化酶表达及激素分泌的影响

目的:研究蛋白日粮水平对断奶仔猪消化的影响机制。
创新点:从营养物质消化及激素变化方面研究低蛋白日粮对仔猪胃肠道消化吸收的影响,探究低蛋白日粮饲养的可行性。
方法:18头28日龄断奶仔猪经过7天适应期后平均体重为(9.57±0.64) kg。仔猪随机分为三组,每组6头,分别饲喂20%、17%和14%粗蛋白日粮并自由采食45天。根据国家研究委员会(NRC)2012年的标准,通过添加赖氨酸、蛋氨酸、半胱氨酸、苏氨酸及色氨酸等理想蛋白模型满足仔猪生长需求。实验结束后,进行颈静脉采血并屠宰取样。
结论:结果表明基于蛋白日粮水平对内分泌及消化酶影响,与14%及20%粗蛋白组相比,17%蛋白日粮更有利于胃肠道和胰腺等组织中三种主要营养物质的消化。因此,17%粗蛋白水平更适合断奶仔猪的营养需求,证实了低蛋白日粮对断奶仔猪饲养的可行性。

关键词:日粮蛋白;仔猪;胃肠道;胰腺;消化;激素

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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