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Received: 2013-03-27

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.12 P.1070-1083


Influence of heat stress on leaf ultrastructure, photosynthetic performance, and ascorbate peroxidase gene expression of two pear cultivars (Pyrus pyrifolia)

Author(s):  Dong-feng Liu, Dong Zhang, Guo-qin Liu, Sayed Hussain, Yuan-wen Teng

Affiliation(s):  State Agricultural Ministry Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Department of Horticulture, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   ywteng@zju.edu.cn

Key Words:  Pear, Ultrastructure, Ascorbate peroxidase, Subcellular localization, Synergistic effect

Dong-feng Liu, Dong Zhang, Guo-qin Liu, Sayed Hussain, Yuan-wen Teng. Influence of heat stress on leaf ultrastructure, photosynthetic performance, and ascorbate peroxidase gene expression of two pear cultivars (Pyrus pyrifolia)[J]. Journal of Zhejiang University Science B, 2013, 14(12): 1070-1083.

@article{title="Influence of heat stress on leaf ultrastructure, photosynthetic performance, and ascorbate peroxidase gene expression of two pear cultivars (Pyrus pyrifolia)",
author="Dong-feng Liu, Dong Zhang, Guo-qin Liu, Sayed Hussain, Yuan-wen Teng",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Influence of heat stress on leaf ultrastructure, photosynthetic performance, and ascorbate peroxidase gene expression of two pear cultivars (Pyrus pyrifolia)
%A Dong-feng Liu
%A Dong Zhang
%A Guo-qin Liu
%A Sayed Hussain
%A Yuan-wen Teng
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 12
%P 1070-1083
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300094

T1 - Influence of heat stress on leaf ultrastructure, photosynthetic performance, and ascorbate peroxidase gene expression of two pear cultivars (Pyrus pyrifolia)
A1 - Dong-feng Liu
A1 - Dong Zhang
A1 - Guo-qin Liu
A1 - Sayed Hussain
A1 - Yuan-wen Teng
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 12
SP - 1070
EP - 1083
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300094

Plants encounter a variety of stresses in natural environments. One-year-old pot-grown trees of pear (Pyrus pyrifolia Nakai cv. Cuiguan and Wonhwang) were exposed to two heat stress regimes. Under constant short-term heat stress, chloroplasts and mitochondria were visibly damaged. Relative chlorophyll content and maximum photochemical efficiency of photosystem II were significantly decreased, which indicated that the leaf photosynthetic capability declined. Under chronic heat stress, mesophyll cell ultrastructure was not obviously damaged, but leaf photosynthetic capability was still restrained. As chronic heat stress was a simulation of the natural environment in summer, further study of the responses under this stress regime was undertaken. ascorbate peroxidase (APX) activity was increased in ‘Cuiguan’, but not in ‘Wonhwang’. Inducible expression of PpAPX genes in the cytoplasm, chloroplasts and peroxisomes was consistent with increased APX activity in ‘Cuiguan’, whereas only weak induction of PpAPX genes was observed in ‘Wonhwang’. The isoenzymes cytosolic APX1 (cAPX1) and stromal APX (sAPX) were confirmed to be localized in the cytoplasm and chloroplasts, respectively.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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