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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.7 P.458~464


Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12-IPT modified gerbera

Author(s):  LAI Qi-xian, BAO Zhi-yi, ZHU Zhu-jun, QIAN Qiong-qiu, MAO Bi-zeng

Affiliation(s):  Key Laboratory of Horticultural Plant Development and Biotechnology, Department of Horticulture, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   zhjzhu@zju.edu.cn

Key Words:  Antioxidant enzymes, Gerbera, Leaf disc, Leaf senescence, Osmotic stress, PSAG12-IPT

LAI Qi-xian, BAO Zhi-yi, ZHU Zhu-jun, QIAN Qiong-qiu, MAO Bi-zeng. Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12-IPT modified gerbera[J]. Journal of Zhejiang University Science B, 2007, 8(7): 458~464.

@article{title="Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12-IPT modified gerbera",
author="LAI Qi-xian, BAO Zhi-yi, ZHU Zhu-jun, QIAN Qiong-qiu, MAO Bi-zeng",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12-IPT modified gerbera
%A LAI Qi-xian
%A BAO Zhi-yi
%A ZHU Zhu-jun
%A QIAN Qiong-qiu
%A MAO Bi-zeng
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 7
%P 458~464
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0458

T1 - Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12-IPT modified gerbera
A1 - LAI Qi-xian
A1 - BAO Zhi-yi
A1 - ZHU Zhu-jun
A1 - QIAN Qiong-qiu
A1 - MAO Bi-zeng
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 7
SP - 458
EP - 464
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0458

leaf senescence is often caused by water deficit and the chimeric gene pSAG12-IPT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance (TBARS) and antioxidant enzymes activities were investigated during leaf senescence of PSAGl2-IPT modified gerbera induced by osmotic stress compared with the control plant (wild type). leaf discs were incubated in 20%, 40% (w/v) polyethylene glycol (PEG) 6 000 nutrient solution for 20 h under continuous light [130 µmol/(m2·s)]. The results showed that the contents of chlorophylls, carotenoids and soluble protein were decreased by osmotic stress with the decrease being more pronounced at 40% PEG, but that, at the same PEG concentration the decrease in the transgenic plants was significantly lower than that in the control plant. The activities of superoxide dismutase (SOD), catalases (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and dehydroascorbate reductase (DHAR) were stimulated by PEG treatment. However, the increases were higher in pSAG12-IPT transgenic plants than in the control plants, particularly at 40% PEG treatment. Lipid peroxidation (TBARS content) was increased by PEG treatment with the increase being much lower in transgenic plant than in the control plant. It could be concluded that the increases in the activities of antioxidant enzymes including SOD, CAT, APX, GPX and DHAR were responsible for the delay of leaf senescence induced by osmotic stress.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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