CLC number: S98; TS254
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2009-07-06
Cited: 30
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Yong-liang ZHUANG, Xue ZHAO, Ba-fang LI. Optimization of antioxidant activity by response surface methodology in hydrolysates of jellyfish (Rhopilema esculentum) umbrella collagen[J]. Journal of Zhejiang University Science B, 2009, 10(8): 572-579.
@article{title="Optimization of antioxidant activity by response surface methodology in hydrolysates of jellyfish (Rhopilema esculentum) umbrella collagen",
author="Yong-liang ZHUANG, Xue ZHAO, Ba-fang LI",
journal="Journal of Zhejiang University Science B",
volume="10",
number="8",
pages="572-579",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0920081"
}
%0 Journal Article
%T Optimization of antioxidant activity by response surface methodology in hydrolysates of jellyfish (Rhopilema esculentum) umbrella collagen
%A Yong-liang ZHUANG
%A Xue ZHAO
%A Ba-fang LI
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 8
%P 572-579
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920081
TY - JOUR
T1 - Optimization of antioxidant activity by response surface methodology in hydrolysates of jellyfish (Rhopilema esculentum) umbrella collagen
A1 - Yong-liang ZHUANG
A1 - Xue ZHAO
A1 - Ba-fang LI
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 8
SP - 572
EP - 579
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920081
Abstract: To optimize the hydrolysis conditions to prepare hydrolysates of jellyfish umbrella collagen with the highest hydroxyl radical scavenging activity, collagen extracted from jellyfish umbrella was hydrolyzed with trypsin, and response surface methodology (RSM) was applied. The optimum conditions obtained from experiments were pH 7.75, temperature (T) 48.77 °C, and enzyme-to-substrate ratio ([E]/[S]) 3.50%. The analysis of variance in RSM showed that pH and [E]/[S] were important factors that significantly affected the process (P<0.05 and P<0.01, respectively). The hydrolysates of jellyfish umbrella collagen were fractionated by high performance liquid chromatography (HPLC), and three fractions (HF-1>3000 Da, 1000 Da<HF-2<3000 Da, and HF-3<1000 Da) were collected. The HF-2 fraction had the highest hydroxyl radical scavenging activity with the highest yield compared with the other two fractions. Furthermore, HF-2 also showed the strongest Cu2+-chelating ability and the best tyrosinase-inhibitory activity.
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