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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.7 P.558~566


Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition

Author(s):  Yao-xing XU, Yan-li LI, Shao-chun XU, Yong LIU, Xin WANG, Jiang-wu TANG

Affiliation(s):  Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Corresponding email(s):   xuyx007@163.com

Key Words:  Xylanase, Optimization of medium composition, Plackett-Burman design (PBD), Central composite design (CCD), Response surface methodology (RSM), Aspergillus niger XY-1

Yao-xing XU, Yan-li LI, Shao-chun XU, Yong LIU, Xin WANG, Jiang-wu TANG. Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition[J]. Journal of Zhejiang University Science B, 2008, 9(7): 558~566.

@article{title="Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition",
author="Yao-xing XU, Yan-li LI, Shao-chun XU, Yong LIU, Xin WANG, Jiang-wu TANG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition
%A Yao-xing XU
%A Yan-li LI
%A Shao-chun XU
%A Yong LIU
%A Jiang-wu TANG
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 7
%P 558~566
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820038

T1 - Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition
A1 - Yao-xing XU
A1 - Yan-li LI
A1 - Shao-chun XU
A1 - Yong LIU
A1 - Xin WANG
A1 - Jiang-wu TANG
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 7
SP - 558
EP - 566
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820038

Objective: To study the optimal medium composition for xylanase production by Aspergillus niger XY-1 in solid-state fermentation (SSF). Methods: Statistical methodology including the plackett-Burman design (PBD) and the central composite design (CCD) was employed to investigate the individual crucial component of the medium that significantly affected the enzyme yield. Results: Firstly, NaNO3, yeast extract, urea, Na2CO3, MgSO4, peptone and (NH4)2SO4 were screened as the significant factors positively affecting the xylanase production by PBD. Secondly, by valuating the nitrogen sources effect, urea was proved to be the most effective and economic nitrogen source for xylanase production and used for further optimization. Finally, the CCD and response surface methodology (RSM) were applied to determine the optimal concentration of each significant variable, which included urea, Na2CO3 and MgSO4. Subsequently a second-order polynomial was determined by multiple regression analysis. The optimum values of the critical components for maximum xylanase production were obtained as follows: x1 (urea)=0.163 (41.63 g/L), x2 (Na2CO3)=−1.68 (2.64 g/L), x3 (MgSO4)=1.338 (10.68 g/L) and the predicted xylanase value was 14374.6 U/g dry substrate. Using the optimized condition, xylanase production by Aspergillus niger XY-1 after 48 h fermentation reached 14637 U/g dry substrate with wheat bran in the shake flask. Conclusion: By using PBD and CCD, we obtained the optimal composition for xylanase production by Aspergillus niger XY-1 in SSF, and the results of no additional expensive medium and shortened fermentation time for higher xylanase production show the potential for industrial utilization.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Bakri, Y., Jacques, P., Thonart, P., 2003. Xylanase production by Penicillium canescens 10-10c in solid-state fermentation. Appl. Biochem. Biotechnol., 108(1-3):737-774.

[2] Bandivadekar, K.R., Deshpande, V.V., 1994. Enhanced stability of cellulase free xylanase from Chainia sp. (NCL. 82.5.1). Biotechnol. Lett., 16(2):179-182.

[3] Barrera, M., Cervantes, M., Sauer, W.C., Araiza, A.B., Torrentera, N., Cervantes, M., 2004. Ileal amino acid digestibility and performance of growing pigs fed wheat-based diets supplemented with xylanase. J. Anim. Sci., 82(7):1997-2003.

[4] Botella, C., Diaz, A., de Ory, I., Webb, C., Blandino, A., 2007. Xylanase and pectinase production by Aspergillus awamori on grape pomace in solid state fermentation. Process Biochemistry, 42(1):98-101.

[5] Box, G., Wilson, K.B., 1951. On the experimental attainment of optimum conditions. J. Roy. Stat., 13(1):1-45.

[6] Courtin, C.M., Gys, W., Delcour, J.A., 2006. Arabinoxylans and endoxylanases in refrigerated dough syruping. J. Sci. Food Agric., 86(11):1587-1595.

[7] de Souza, D.F., de Souza, C.G.M., Peralta, R.M., 2001. Effect of easily metabolizable sugars in the production of xylanase by Aspergillus tamari in solid-state fermentation. Proc. Biochem., 36(8-9):835-838.

[8] Gessesse, A., Mamo, G., 1999. High-level xylanase production by an alkaliphilic Bacillus sp. by using solid-state fermentation. Enzyme and Microbial Technology, 25(1-2):68-72.

[9] Ghanem, N.B., Yusef, H.H., Mahrouse, H.K., 2000. Production of Aspergillus terreus xylanase in solid-state cultures: application of the Plackett-Burman experimental design to evaluate nutritional requirement. Bioresource Technology, 73(2):113-121.

[10] Haaland, P.D. (Ed.), 1989. Separating Signals from the Noise. In: Experimental Design in Biotechnology. Marcel Dekker, New York, p.61-83.

[11] Heck, J.X., Flores, S.H., Hertz, P.F., Ayub, M.A.Z., 2006. Statistical optimization of thermo-tolerant xylanase activity from Amazon isolated Bacillus circulans on solid-state cultivation. Bioresource Technology, 97(15):1902-1906.

[12] Li, Y., Cui, F.J., Liu, Z.Q., Xu, Y.Y., Zhao, H., 2007a. Improvement of xylanase production by Penicillium oxalicum ZH-30 using response surface methodology. Enzyme and Microbial Technology, 40(5):1381-1388.

[13] Li, Y., Liu, Z.Q., Zhao, H., Xu, Y.Y., Cui, F.J., 2007b. Statistical optimization of xylanase production from new isolated Penicillium oxalicum ZH-30 in submerged fermentation. Biochemical Engineering Journal, 34(1):82-86.

[14] Li, Y., Liu, Z.Q., Cui, F.J., Xu, Y.Y., Zhao, H., 2007c. Application of experimental design to optimize culture requirements of Aspergillus sp. Zh-26 producing xylanase for degradation of arabinoxylans in mashing. Journal of Food Science, 72(5):E320-E329.

[15] Li, Y., Liu, Z.Q., Cui, F.J., Xu, Y.Y., Zhao, H., 2007d. Application of Plackett-Burman experimental design and Doehlert design to evaluate nutritional requirements for xylanase production by Alternaria mali ND-16. Appl. Microbiol. Biotechnol., 77(2):285-291.

[16] Lindberg, J.E., Lyberg, K., Sands, J., 2007. Influence of phytase and xylanase supplementation of a wheat-based diet on ileal and total tract digestibility in growing pigs. Livestock Science, 109(1-3):268-270.

[17] Narang, S., Sahai, V., Bisaria, V.S., 2001. Optimization of xylanase production by Melanocarpus albomyces IIS-68 in solid state fermentation using response surface methodology. J. Biosci. Bioeng., 91(4):425-427.

[18] Nortey, T.N., Patience, J.F., Sands, J.S., Zijlstra, R.T., 2007. Xylanase supplementation improves energy digestibility of wheat by-products in grower pigs. Livestock Science, 109(1-3):96-99.

[19] Park, Y.S., Kang, S.W., Lee, J.S., Hong, S.I., Kim, S.W., 2002. Xylanase production in solid state fermentation by Aspergillus niger mutant using statistical experimental designs. Appl. Microbiol. Biotechnol., 58(6):761-766.

[20] Plackett, R.L., Burman, J.P., 1946. The design of optimum multifactorial experiments. Biometrika, 33(4):305-325.

[21] Sanchez, V.E., Pilosof, A.M.R., 2000. Protease-conidia relationships of Aspergillus niger grown in solid state fermentation. Biotechnol. Lett., 22(20):1629-1633.

[22] Senthilkumar, S.R., Ashokkumar, B., Chandra Raj, K., Gunasekaran, P., 2005. Optimization of medium composition for alkali-stable xylanase production by Aspergillus fischeri Fxn 1 in solid-state fermentation using central composite rotary design. Bioresource Technology, 96(12):1380-1386.

[23] Slominski, B.A., Boros, D., Campbell, L.D., Guenter, W., Jones, O., 2004. Wheat by-products in poultry nutrition. Part I. Chemical and nutritive composition of wheat screenings, bakery by-products and wheat mill run. Can. J. Anim. Sci., 84:421-428.

[24] Sonia, K.G., Chadha, B.S., Saini, H.S., 2005. Sorghum straw for xylanase hyper-production by Thermomyces lanuginosus (D2W3) under solid-state fermentation. Bioresource Technology, 96(14):1561-1569.

[25] Sterk, A., Verdonk, J.M.A.J., Mul, A.J., Soenen, B., Bezençon, M.L., Frehner, M., Losa, R., 2007. Effect of xylanase supplementation to a cereal-based diet on the apparent faecal digestibility in weanling piglets. Livestock Science, 108(1-3):269-271.

[26] Sun, Z.T., Zhao, X.Y., Liu, J.J., Du, J.H., 2007. Microbial xylanases and their industrial applications. Biotechnology, 17(2):93-97.

[27] Tapingkae, W., Yachai, M., Visessanguan, W., Pongtanya, P., Pongpiachan, P., 2008. Influence of crude xylanase from Aspergillus niger FAS128 on the in vitro digestibility and production performance of piglets. Anim. Feed Sci., Technol., 140(1-2):125-138

[28] Wu, M., Li, S.C., Yao, J.M., Pan, R.R., Yu, Z.L., 2005. Mutant of a xylanase-producing strain of Aspergillus niger in solid state fermentation by low energy ion implantation. World Journal of Microbiology Biotechnology, 21(6-7):1045-1049.

[29] Yang, S.Q., Yan, Q.J., Jiang, Z.Q., Li, L.T., Tian, H.M., Wang, Y.Z., 2006. High-level of xylanase production by the thermophilic Paecilomyces themophila J18 on wheat straw in solid-state fermentation. Bioresource Technology, 97(15):1794-1800.

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