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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.7 P.558~566

http://doi.org/10.1631/jzus.B0820038


Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition


Author(s):  Yao-xing XU, Yan-li LI, Shao-chun XU, Yong LIU, Xin WANG, Jiang-wu TANG

Affiliation(s):  Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Corresponding email(s):   xuyx007@163.com

Key Words:  Xylanase, Optimization of medium composition, Plackett-Burman design (PBD), Central composite design (CCD), Response surface methodology (RSM), Aspergillus niger XY-1


Yao-xing XU, Yan-li LI, Shao-chun XU, Yong LIU, Xin WANG, Jiang-wu TANG. Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition[J]. Journal of Zhejiang University Science B, 2008, 9(7): 558~566.

@article{title="Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition",
author="Yao-xing XU, Yan-li LI, Shao-chun XU, Yong LIU, Xin WANG, Jiang-wu TANG",
journal="Journal of Zhejiang University Science B",
volume="9",
number="7",
pages="558~566",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820038"
}

%0 Journal Article
%T Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition
%A Yao-xing XU
%A Yan-li LI
%A Shao-chun XU
%A Yong LIU
%A Xin WANG
%A Jiang-wu TANG
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 7
%P 558~566
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820038

TY - JOUR
T1 - Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition
A1 - Yao-xing XU
A1 - Yan-li LI
A1 - Shao-chun XU
A1 - Yong LIU
A1 - Xin WANG
A1 - Jiang-wu TANG
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 7
SP - 558
EP - 566
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820038


Abstract: 
Objective: To study the optimal medium composition for xylanase production by Aspergillus niger XY-1 in solid-state fermentation (SSF). Methods: Statistical methodology including the plackett-Burman design (PBD) and the central composite design (CCD) was employed to investigate the individual crucial component of the medium that significantly affected the enzyme yield. Results: Firstly, NaNO3, yeast extract, urea, Na2CO3, MgSO4, peptone and (NH4)2SO4 were screened as the significant factors positively affecting the xylanase production by PBD. Secondly, by valuating the nitrogen sources effect, urea was proved to be the most effective and economic nitrogen source for xylanase production and used for further optimization. Finally, the CCD and response surface methodology (RSM) were applied to determine the optimal concentration of each significant variable, which included urea, Na2CO3 and MgSO4. Subsequently a second-order polynomial was determined by multiple regression analysis. The optimum values of the critical components for maximum xylanase production were obtained as follows: x1 (urea)=0.163 (41.63 g/L), x2 (Na2CO3)=−1.68 (2.64 g/L), x3 (MgSO4)=1.338 (10.68 g/L) and the predicted xylanase value was 14374.6 U/g dry substrate. Using the optimized condition, xylanase production by Aspergillus niger XY-1 after 48 h fermentation reached 14637 U/g dry substrate with wheat bran in the shake flask. Conclusion: By using PBD and CCD, we obtained the optimal composition for xylanase production by Aspergillus niger XY-1 in SSF, and the results of no additional expensive medium and shortened fermentation time for higher xylanase production show the potential for industrial utilization.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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