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On-line Access: 2013-12-03

Received: 2013-04-08

Revision Accepted: 2013-08-09

Crosschecked: 2013-11-25

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.12 P.1110-1120


Cloning and functional analysis of a novel ascorbate peroxidase (APX) gene from Anthurium andraeanum

Author(s):  Hui-chun Liu, Dan-qing Tian, Jian-xin Liu, Guang-ying Ma, Qing-cheng Zou, Zhu-jun Zhu

Affiliation(s):  College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   zhjzhu@zju.edu.cn

Key Words:  AnAPX, Gene expression, Cold stress, Anthurium andraeanum

Hui-chun Liu, Dan-qing Tian, Jian-xin Liu, Guang-ying Ma, Qing-cheng Zou, Zhu-jun Zhu. Cloning and functional analysis of a novel ascorbate peroxidase (APX) gene from Anthurium andraeanum[J]. Journal of Zhejiang University Science B, 2013, 14(12): 1110-1120.

@article{title="Cloning and functional analysis of a novel ascorbate peroxidase (APX) gene from Anthurium andraeanum",
author="Hui-chun Liu, Dan-qing Tian, Jian-xin Liu, Guang-ying Ma, Qing-cheng Zou, Zhu-jun Zhu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Cloning and functional analysis of a novel ascorbate peroxidase (APX) gene from Anthurium andraeanum
%A Hui-chun Liu
%A Dan-qing Tian
%A Jian-xin Liu
%A Guang-ying Ma
%A Qing-cheng Zou
%A Zhu-jun Zhu
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 12
%P 1110-1120
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300105

T1 - Cloning and functional analysis of a novel ascorbate peroxidase (APX) gene from Anthurium andraeanum
A1 - Hui-chun Liu
A1 - Dan-qing Tian
A1 - Jian-xin Liu
A1 - Guang-ying Ma
A1 - Qing-cheng Zou
A1 - Zhu-jun Zhu
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 12
SP - 1110
EP - 1120
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300105

An 888-bp full-length ascorbate peroxidase (APX) complementary DNA (cDNA) gene was cloned from Anthurium andraeanum, and designated as AnAPX. It contains a 110-bp 5′-noncoding region, a 28-bp 3′-noncoding region, and a 750-bp open reading frame (ORF). This protein is hydrophilic with an aliphatic index of 81.64 and its structure consisting of α-helixes, β-turns, and random coils. The AnAPX protein showed 93%, 87%, 87%, 87%, and 86% similarities to the APX homologs from Zantedeschia aethiopica, Vitis pseudoreticulata, Gossypium hirsutum, Elaeis guineensis, and Zea mays, respectively. AnAPX gene transcript was measured non-significantly in roots, stems, leaves, spathes, and spadices by real-time polymerase chain reaction (RT-PCR) analysis. Interestingly, this gene expression was remarkably up-regulated in response to a cold stress under 6 °C, implying that AnAPX might play an important role in A. andraeanum tolerance to cold stress. To confirm this function we overexpressed AnAPX in tobacco plants by transformation with an AnAPX expression construct driven by CaMV 35S promoter. The transformed tobacco seedlings under 4 °C showed less electrolyte leakage (EL) and malondialdehyde (MDA) content than the control. The content of MDA was correlated with chilling tolerance in these transgenic plants. These results show that AnAPX can prevent the chilling challenged plant from cell membrane damage and ultimately enhance the plant cold tolerance.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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