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On-line Access: 2013-12-03

Received: 2013-07-02

Revision Accepted: 2013-10-18

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.12 P.1121-1131


Involvement of phosphatidate phosphatase in the biosynthesis of triacylglycerols in Chlamydomonas reinhardtii

Author(s):  Xiao-dong Deng, Jia-jia Cai, Xiao-wen Fei

Affiliation(s):  Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; more

Corresponding email(s):   Feixw2000@hotmail.com

Key Words:  Phosphatidate phosphohydrolase 2, Triacylglycerol biosynthesis, RNAi, Chlamydomonas reinhardtii, Nitrogen deprivation, Over-expressionThe online version of this article contains supplementary materials Data S1

Xiao-dong Deng, Jia-jia Cai, Xiao-wen Fei. Involvement of phosphatidate phosphatase in the biosynthesis of triacylglycerols in Chlamydomonas reinhardtii[J]. Journal of Zhejiang University Science B, 2013, 14(12): 1121-1131.

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author="Xiao-dong Deng, Jia-jia Cai, Xiao-wen Fei",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Involvement of phosphatidate phosphatase in the biosynthesis of triacylglycerols in Chlamydomonas reinhardtii
%A Xiao-dong Deng
%A Jia-jia Cai
%A Xiao-wen Fei
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 12
%P 1121-1131
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300180

T1 - Involvement of phosphatidate phosphatase in the biosynthesis of triacylglycerols in Chlamydomonas reinhardtii
A1 - Xiao-dong Deng
A1 - Jia-jia Cai
A1 - Xiao-wen Fei
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 12
SP - 1121
EP - 1131
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300180

Lipid biosynthesis is essential for eukaryotic cells, but the mechanisms of the process in microalgae remain poorly understood. Phosphatidic acid phosphohydrolase or 3-sn-phosphatidate phosphohydrolase (PAP) catalyzes the dephosphorylation of phosphatidic acid to form diacylglycerols and inorganic orthophosphates. This reaction is integral in the synthesis of triacylglycerols. In this study, the mRNA level of the PAP isoform CrPAP2 in a species of Chlamydomonas was found to increase in nitrogen-free conditions. Silencing of the CrPAP2 gene using RNA interference resulted in the decline of lipid content by 2.4%–17.4%. By contrast, over-expression of the CrPAP2 gene resulted in an increase in lipid content by 7.5%–21.8%. These observations indicate that regulation of the CrPAP2 gene can control the lipid content of the algal cells. In vitro CrPAP2 enzyme activity assay indicated that the cloned CrPAP2 gene exhibited biological activities.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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