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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.12 P.1132-1143

http://doi.org/10.1631/jzus.B1200126


Morphological and biochemical responses of Oryza sativa L. (cultivar MR219) to ion beam irradiation


Author(s):  Anna Pick Kiong Ling, Ying Chian Ung, Sobri Hussein, Abdul Rahim Harun, Atsushi Tanaka, Hase Yoshihiro

Affiliation(s):  Division of Human Biology, International Medical University (IMU), 57000 Kuala Lumpur, Malaysia; more

Corresponding email(s):   anna_ling@imu.edu.my

Key Words:  In vitro mutagenesis, Ion beam irradiation, Total chlorophyll content, Total soluble protein content, Mutation breeding


Anna Pick Kiong Ling, Ying Chian Ung, Sobri Hussein, Abdul Rahim Harun, Atsushi Tanaka, Hase Yoshihiro. Morphological and biochemical responses of Oryza sativa L. (cultivar MR219) to ion beam irradiation[J]. Journal of Zhejiang University Science B, 2013, 14(12): 1132-1143.

@article{title="Morphological and biochemical responses of Oryza sativa L. (cultivar MR219) to ion beam irradiation",
author="Anna Pick Kiong Ling, Ying Chian Ung, Sobri Hussein, Abdul Rahim Harun, Atsushi Tanaka, Hase Yoshihiro",
journal="Journal of Zhejiang University Science B",
volume="14",
number="12",
pages="1132-1143",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200126"
}

%0 Journal Article
%T Morphological and biochemical responses of Oryza sativa L. (cultivar MR219) to ion beam irradiation
%A Anna Pick Kiong Ling
%A Ying Chian Ung
%A Sobri Hussein
%A Abdul Rahim Harun
%A Atsushi Tanaka
%A Hase Yoshihiro
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 12
%P 1132-1143
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200126

TY - JOUR
T1 - Morphological and biochemical responses of Oryza sativa L. (cultivar MR219) to ion beam irradiation
A1 - Anna Pick Kiong Ling
A1 - Ying Chian Ung
A1 - Sobri Hussein
A1 - Abdul Rahim Harun
A1 - Atsushi Tanaka
A1 - Hase Yoshihiro
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 12
SP - 1132
EP - 1143
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200126


Abstract: 
Objective: Heavy ion beam, which has emerged as a new mutagen in the mutation breeding of crops and ornamental plants, is expected to result in the induction of novel mutations. This study investigates the morphological and biochemical responses of Oryza sativa toward different doses of carbon ion beam irradiation. Methods: In this study, the dry seeds of O. sativa were irradiated at 0, 20, 40, 60, 80, 100, and 120 Gy, followed by in-vitro germination under controlled conditions. Morphological and biochemical studies were conducted to investigate the morphological and physiological responses of O. sativa towards ion beam irradiation. Results: The study demonstrated that low doses (10 Gy) of ion beam have a stimulating effect on the height, root length, and fresh weight of the plantlets but not on the number of leaves. Meanwhile, doses higher than 10 Gy caused reductions in all the morphological parameters studied as compared to the control samples. The highest total soluble protein content [(2.11±0.47) mg/g FW] was observed in plantlets irradiated at 20 Gy. All irradiated plantlets were found to have 0.85% to 58.32% higher specific activity of peroxidase as compared to the control samples. The present study also revealed that low doses of ion beam (10 and 20 Gy) had negligible effect on the total chlorophyll content of O. sativa plantlets while 40 Gy had a stimulating effect on the chlorophyll content. Plantlets irradiated between 40 to 120 Gy were shown to be 0.38% to 9.98% higher in total soluble nitrogen content which, however, was not significantly different from the control samples. Conclusions: Carbon ion beam irradiation administered at low to moderate doses of 10 to 40 Gy may induce O. sativa mutants with superior characteristics.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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