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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.8 P.803-811


Isolation and physiological characteristics of a premature senescence mutant in rice (Oryza sativa L.)

Author(s):  LI Fu-zhen, JIN Song-heng, HU Guo-cheng, FU Ya-ping, SI Hua-min, JIANG De-an, SUN Zong-xiu

Affiliation(s):  State Key Laboratory of Plant Physiology and Biochemistry, School of Life Sciences, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   dajiang@zju.edu.cn, sunzx@hzcnc.com

Key Words:  Antioxidative enzymes, Net photosynthesis rate, Peptidase, Phytotron, Premature senescence mutant, Rice (Oryza sativa L.)

LI Fu-zhen, JIN Song-heng, HU Guo-cheng, FU Ya-ping, SI Hua-min, JIANG De-an, SUN Zong-xiu. Isolation and physiological characteristics of a premature senescence mutant in rice (Oryza sativa L.)[J]. Journal of Zhejiang University Science B, 2005, 6(8): 803-811.

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author="LI Fu-zhen, JIN Song-heng, HU Guo-cheng, FU Ya-ping, SI Hua-min, JIANG De-an, SUN Zong-xiu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Isolation and physiological characteristics of a premature senescence mutant in rice (Oryza sativa L.)
%A LI Fu-zhen
%A JIN Song-heng
%A HU Guo-cheng
%A FU Ya-ping
%A SI Hua-min
%A JIANG De-an
%A SUN Zong-xiu
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 8
%P 803-811
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0803

T1 - Isolation and physiological characteristics of a premature senescence mutant in rice (Oryza sativa L.)
A1 - LI Fu-zhen
A1 - JIN Song-heng
A1 - HU Guo-cheng
A1 - FU Ya-ping
A1 - SI Hua-min
A1 - JIANG De-an
A1 - SUN Zong-xiu
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 8
SP - 803
EP - 811
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0803

A rice pse(t) (premature senescence, tentatively) mutant line, was isolated from 4 500 independent T-DNA inserted transgenic lines. The symptoms of premature senescence appeared more severely than those of the control plants (Zhonghua 11, japonica) at the last development stage. To characterize the mutant and provide basic information on the candidate genes by mapping to a physical region of 220-kb, experiments were carried out in two phytotrons under controlled temperature of 24 °C and 28 °C, respectively. The content of chlorophyll, soluble protein and MDA (malondialdehyde), net photosynthesis, the antioxidant enzyme activities of SOD (superoxide dismuase) (EC and POD (peroxidase) (EC and the peptidase activities of leaves were measured from top to bottom according to the leaf positions at the flowering stage. Compared with the control plant, the mutant showed the following characteristics: (1) Higher net photosynthesis rate (Pn) appeared in the 1st and 2nd leaves, contents of chlorophyll and soluble protein were also higher in the 1st leaf; (2) The activities of SOD, POD and peptidase were higher according to the leaf position from top to bottom; (3) The symptom of premature senescence was accelerated in the mutant at 28 °C treatment. The MDA content and the SOD and POD activities between the 24 °C and 28 °C treatment mutants were not significantly different. Content of chlorophyll and soluble protein of leaves mutant decreased rapidly at 28 °C treatment. The results show that pse(t) is sensitive to high temperature. The probable function of PSE(T) is discussed.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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