Full Text:   <685>

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Suppl. Mater.: 

CLC number: S335.3

On-line Access: 2018-04-04

Received: 2017-07-03

Revision Accepted: 2017-10-18

Crosschecked: 2018-02-10

Cited: 0

Clicked: 2109

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Min-tao Zhong

https://orcid.org/0000-0002-2190-1153

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.4 P.263-273

http://doi.org/10.1631/jzus.B1700337


PGL3 is required for chlorophyll synthesis and impacts leaf senescence in rice


Author(s):  Jing Ye, Yao-Long Yang, Xing-Hua Wei, Xiao-Jun Niu, Shan Wang, Qun Xu, Xiao-Ping Yuan, Han-Yong Yu, Yi-Ping Wang, Yue Feng, Shu Wang

Affiliation(s):  College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China; more

Corresponding email(s):   fy_555500@163.com, wangshusl@126.com

Key Words:  Pale-green leaf, Chlorophyll synthesis, Reactive oxygen species, Senescence, Rice


Jing Ye, Yao-Long Yang, Xing-Hua Wei, Xiao-Jun Niu, Shan Wang, Qun Xu, Xiao-Ping Yuan, Han-Yong Yu, Yi-Ping Wang, Yue Feng, Shu Wang. PGL3 is required for chlorophyll synthesis and impacts leaf senescence in rice[J]. Journal of Zhejiang University Science B, 2018, 19(4): 263-273.

@article{title="PGL3 is required for chlorophyll synthesis and impacts leaf senescence in rice",
author="Jing Ye, Yao-Long Yang, Xing-Hua Wei, Xiao-Jun Niu, Shan Wang, Qun Xu, Xiao-Ping Yuan, Han-Yong Yu, Yi-Ping Wang, Yue Feng, Shu Wang",
journal="Journal of Zhejiang University Science B",
volume="19",
number="4",
pages="263-273",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700337"
}

%0 Journal Article
%T PGL3 is required for chlorophyll synthesis and impacts leaf senescence in rice
%A Jing Ye
%A Yao-Long Yang
%A Xing-Hua Wei
%A Xiao-Jun Niu
%A Shan Wang
%A Qun Xu
%A Xiao-Ping Yuan
%A Han-Yong Yu
%A Yi-Ping Wang
%A Yue Feng
%A Shu Wang
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 4
%P 263-273
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700337

TY - JOUR
T1 - PGL3 is required for chlorophyll synthesis and impacts leaf senescence in rice
A1 - Jing Ye
A1 - Yao-Long Yang
A1 - Xing-Hua Wei
A1 - Xiao-Jun Niu
A1 - Shan Wang
A1 - Qun Xu
A1 - Xiao-Ping Yuan
A1 - Han-Yong Yu
A1 - Yi-Ping Wang
A1 - Yue Feng
A1 - Shu Wang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 4
SP - 263
EP - 273
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700337


Abstract: 
rice leaf color mutants play a great role in research about the formation and development of chloroplasts and the genetic mechanism of the chlorophyll (Chl) metabolism pathway. pgl3 is a rice leaf color mutant derived from Xiushui11 (Oryza sativa L. spp. japonica), treated with ethyl methane sulfonate (EMS). The mutant exhibited a pale-green leaf (pgl) phenotype throughout the whole development as well as reduced grain quality. Map-based cloning of PGL3 revealed that it encodes the chloroplast signal recognition particle 43 kDa protein (cpSRP43). PGL3 affected the Chl synthesis by regulating the expression levels of the Chl synthesis-associated genes. Considerable reactive oxygen species were accumulated in the leaves of pgl3, and the transcription levels of its scavenging genes were down-regulated, indicating that pgl3 can accelerate senescence. In addition, high temperatures could inhibit the plant’s growth and facilitate the process of senescence in pgl3.

PGL3对水稻叶绿素合成和叶片衰老的影响

目的:研究PGL3的遗传机制与生物学功能.
创新点:研究一个水稻叶色突变体的鉴定与基因克隆,并探讨其对叶绿素合成和叶片衰老的影响.
方法:通过乙基甲磺酸(EMS)诱变,获得了一个淡绿叶突变体pgl3,运用图位克隆法对PGL3进行定位,并对PGL3的功能进行研究.
结论:PGL3通过调节叶绿素合成相关基因的表达水平影响叶绿素合成.pgl3叶片中清除活性氧基因转录水平下调,导致活性氧大量积累,表明pgl3可以加速衰老.此外,高温可以抑制植物的生长并加速pgl3衰老.

关键词:淡绿叶;叶绿素合成;活性氧;衰老;水稻

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[44]List of electronic supplementary materials

[45]Table S1 Molecular markers used for mapping of the mutation

[46]Table S2 List of genes used for real-time PCR analysis

[47]Fig. S1 Rice quality traits in WT and pgl3

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