Full Text:   <658>

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Suppl. Mater.: 

CLC number: Q939.46

On-line Access: 2018-04-04

Received: 2017-03-28

Revision Accepted: 2017-05-11

Crosschecked: 2018-03-12

Cited: 0

Clicked: 1516

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Yong-chao Li

https://orcid.org/0000-0002-8778-0972

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.4 P.274-284

10.1631/jzus.B1700171


A novel endogenous badnavirus exists in Alhagi sparsifolia


Author(s):  Yong-chao Li, Jian-guo Shen, Guo-huan Zhao, Qin Yao, Wei-min Li

Affiliation(s):  Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China; more

Corresponding email(s):   yaoqin@ujs.edu.cn, liweimin01@caas.cn

Key Words:  Badnavirus, Endogenous Alhagi bacilliform virus, Nuclear integration


Yong-chao Li, Jian-guo Shen, Guo-huan Zhao, Qin Yao, Wei-min Li. A novel endogenous badnavirus exists in Alhagi sparsifolia[J]. Journal of Zhejiang University Science B, 2018, 19(4): 274-284.

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author="Yong-chao Li, Jian-guo Shen, Guo-huan Zhao, Qin Yao, Wei-min Li",
journal="Journal of Zhejiang University Science B",
volume="19",
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pages="274-284",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700171"
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%T A novel endogenous badnavirus exists in Alhagi sparsifolia
%A Yong-chao Li
%A Jian-guo Shen
%A Guo-huan Zhao
%A Qin Yao
%A Wei-min Li
%J Journal of Zhejiang University SCIENCE B
%V 19
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%P 274-284
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700171

TY - JOUR
T1 - A novel endogenous badnavirus exists in Alhagi sparsifolia
A1 - Yong-chao Li
A1 - Jian-guo Shen
A1 - Guo-huan Zhao
A1 - Qin Yao
A1 - Wei-min Li
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 4
SP - 274
EP - 284
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700171


Abstract: 
We report the recovery of a 7068-nt viral sequence from the “viral fossils” embedded in the genome of Alhagi sparsifolia, a typical desert plant. Although the full viral genome remains to be completed, the putative genome structure, the deduced amino acids and phylogenetic analysis unambiguously demonstrate that this viral sequence represents a novel species of the genus Badnavirus. The putative virus is tentatively termed Alhagi bacilliform virus (ABV). Southern blotting and inverse polymerase chain reaction (PCR) data indicate that the ABV-related sequence is integrated into the A. sparsifolia genome, and probably does not give rise to functional episomal virus. Molecular evidence that the ABV sequence exists widely in A. sparsifolia is also presented. To our knowledge, this is the first endogenous badnavirus identified from plants in the Gobi desert, and may provide new clues on the evolution, geographical distribution as well as the host range of the badnaviruses.

疏叶骆驼刺中包含一种新杆状DNA病毒

目的:新杆状DNA病毒的分离与鉴定.
创新点:首次在高寒地区代表性植物--疏叶骆驼刺中发现杆状DNA病毒,为研究杆状DNA病毒的进化、地理分布及寄主范围提供了新证据.
方法:利用分段聚合酶链式反应(PCR)克隆疏叶骆驼刺杆状病毒(Alhagi bacilliform virus,ABV)的基因组序列;通过基因组分析、序列比对和进化树分析阐明ABV的进化地位;用Southern印迹杂交和反向PCR分析ABV序列与宿主基因组的关系;并通过PCR检测确定ABV在我国西北地区疏叶骆驼刺中的分布.
结论:本研究获得了7068 nt 的ABV基因组序列,根据基因组结构、保守序列比对及进化树分析,推测ABV是一种新杆状DNA病毒.分子检测证据表明,ABV基因组序列已整合进入疏叶骆驼刺基因组中,但没有产生游离病毒.此外,对我国西北11个不同地区的疏叶骆驼刺进行PCR检测,结果显示其中9个地区的疏叶骆驼刺均含有ABV序列,由此表明ABV在我国西北地区的疏叶骆驼刺中广泛存在.

关键词:杆状DNA病毒;内生疏叶骆驼刺杆状病毒;基因组整合

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[38]List of electronic supplementary materials

[39]Table S1 Primers used in this study

[40]Table S2 Badnaviruses species for phylogenetic analysis

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