Full Text:   <2934>

CLC number: Q93; R511

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 2011-04-06

Cited: 3

Clicked: 6708

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.5 P.335-345

http://doi.org/10.1631/jzus.B1000336


Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells


Author(s):  Yih-Yih Kok, Wan-Loy Chu, Siew-Moi Phang, Shar Mariam Mohamed, Rakesh Naidu, Pey-Jiun Lai, Shui-Nyuk Ling, Joon-Wah Mak, Patricia Kim-Chooi Lim, Pauline Balraj, Alan Soo-Beng Khoo

Affiliation(s):  Division of Human Biology, International Medical University, 57000 Kuala Lumpur, Malaysia, Institute of Biological Sciences, Faculty of Science, and Institute of Ocean and Earth Sciences (IOES), University of Malaya, 50603 Kuala Lumpur, Malaysia, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia, School of Medicine and Health Sciences, Monash University, 46150 Petaling Jaya, Selangor, Malaysia, Molecular Pathology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia

Corresponding email(s):   wanloy_chu@imu.edu.my

Key Words:  Microalgae, Ankistrodesmus convolutus, Synechococcus elongatus, Spirulina platensis, Lymphoblastoid cells, Epstein-Barr virus (EBV)


Share this article to: More |Next Article >>>

Yih-Yih Kok, Wan-Loy Chu, Siew-Moi Phang, Shar Mariam Mohamed, Rakesh Naidu, Pey-Jiun Lai, Shui-Nyuk Ling, Joon-Wah Mak, Patricia Kim-Chooi Lim, Pauline Balraj, Alan Soo-Beng Khoo. Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells[J]. Journal of Zhejiang University Science B, 2011, 12(5): 335-345.

@article{title="Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells",
author="Yih-Yih Kok, Wan-Loy Chu, Siew-Moi Phang, Shar Mariam Mohamed, Rakesh Naidu, Pey-Jiun Lai, Shui-Nyuk Ling, Joon-Wah Mak, Patricia Kim-Chooi Lim, Pauline Balraj, Alan Soo-Beng Khoo",
journal="Journal of Zhejiang University Science B",
volume="12",
number="5",
pages="335-345",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000336"
}

%0 Journal Article
%T Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells
%A Yih-Yih Kok
%A Wan-Loy Chu
%A Siew-Moi Phang
%A Shar Mariam Mohamed
%A Rakesh Naidu
%A Pey-Jiun Lai
%A Shui-Nyuk Ling
%A Joon-Wah Mak
%A Patricia Kim-Chooi Lim
%A Pauline Balraj
%A Alan Soo-Beng Khoo
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 5
%P 335-345
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000336

TY - JOUR
T1 - Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells
A1 - Yih-Yih Kok
A1 - Wan-Loy Chu
A1 - Siew-Moi Phang
A1 - Shar Mariam Mohamed
A1 - Rakesh Naidu
A1 - Pey-Jiun Lai
A1 - Shui-Nyuk Ling
A1 - Joon-Wah Mak
A1 - Patricia Kim-Chooi Lim
A1 - Pauline Balraj
A1 - Alan Soo-Beng Khoo
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 5
SP - 335
EP - 345
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000336


Abstract: 
This study aimed to assess the inhibitory activities of methanol extracts from the microalgae Ankistrodesmus convolutus, Synechococcus elongatus, and Spirulina platensis against epstein-Barr virus (EBV) in three Burkitt’s lymphoma (BL) cell lines, namely Akata, B95-8, and P3HR-1. The antiviral activity was assessed by quantifying the cell-free EBV DNA using real-time polymerase chain reaction (PCR) technique. The methanol extracts from Ankistrodesmus convolutus and Synechococcus elongatus displayed low cytotoxicity and potent effect in reducing cell-free EBV DNA (EC50<0.01 µg/ml) with a high therapeutic index (>28000). After fractionation by column chromatography, the fraction from Synechococcus elongatus (SEF1) reduced the cell-free EBV DNA most effectively (EC50=2.9 µg/ml, therapeutic index>69). Upon further fractionation by high performance liquid chromatography (HPLC), the sub-fraction SEF1’a was most active in reducing the cell-free EBV DNA (EC50=1.38 µg/ml, therapeutic index>14.5). This study suggests that microalgae could be a potential source of antiviral compounds that can be used against EBV.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Adamson, A.L., Darr, D., Holley-Guthrie, E., Johnson, R.A., Mauser, A., Swenson, J., Kenney, S., 2000. Epstein-Barr virus immediate-early proteins BZLF1 and BRLF1 activate the ATF2 transcription factor by increasing the levels of phosphorylated p38 and c-Jun N-terminal kinases. J. Virol., 74(3):1224-1233.

[2]Akihisa, T., Taguchi, Y., Yasukawa, K., Tokuda, H., Akazawa, H., Suzuki, T., Kimura, Y., 2006. Acerogenin M, a cyclic diarylheptanoid, and other phenolic compounds from Acer nikoense and their anti-inflammatory and anti-tumor-promoting effects. Chem. Pharm. Bull., 54(5):735-739.

[3]Ballout, M., Germi, R., Fafi-Kremer, S., Guimet, J., Bargues, G., Seigneurin, J., Morand, P., 2007. Real-time quantitative PCR for assessment of antiviral drug effects against Epstein-Barr virus replication and EBV late mRNA expression. J. Virol. Methods, 143(1):38-44.

[4]Buck, C.B., Thompson, C.D., Roberts, J.N., Müller, M., Lowy, D.R., Schiller, J.T., 2006. Carrageenan is a potent inhibitor of papillomavirus infection. PLoS Pathogens, 2(7):e69.

[5]Chang, Y., Tung, C.H., Huang, Y.T., Lu, J., Chen, J.Y., Tsai, C.H., 1999. Requirement of cell to cell contact in EBV infection of NPC and keratinocytes. J. Virol., 73(10):8857-8866.

[6]Chêne, A., Donati, D., Guerreriro-Cacais, A.O., Levitsky, V., Chen, Q., Falk, K.I., Orem, J., Kironde, F., Wahlgren, M., Bejarano, M.T., 2007. A molecular link between malaria and Epstein-Barr virus reactivation. PLoS Pathogens, 3(6):e80.

[7]Chu, W.L., Phang, S.M., Goh, S.H., 1995. Influence of carbon source on the growth, biochemical composition and pigmentation of Ankistrodesmus convolutus. J. Appl. Phycol., 7(1):59-64.

[8]Daibata, M., Bandobashi, K., Kuroda, M., Imai, S., Miyoshi, I., Taguchi, H., 2005. Induction of lytic Epstein-Barr virus (EBV) infection by synergistic action of rituximab and dexamethasone renders EBV-positive lymphoma cells more susceptible to ganciclovir cytotoxicity in vitro and in vivo. J. Virol., 79(9):5875-5879.

[9]Fang, C.Y., Lee, C.H., Wu, C.C., Chang, Y.T., Yu, S.L., Chou, S.P., Huang, P.T., Chen, C.L., Hou, J.W., Chang, Y., et al., 2009. Recurrent chemical reactivations of EBV promotes genome instability and enhances tumor progression of nasopharyngeal carcinoma cells. Int. J. Cancer, 124(9):2016-2025.

[10]Feng, P., Ren, E.C., Liu, D., Chan, S.H., Hu, H., 2000. Expression of Epstein-Barr virus lytic gene BRLF1 in nasopharyngeal carcinoma: potential use in diagnosis. J. Gen. Virol., 81(Pt 10):2417-2423.

[11]Gershburg, E., Pagano, J.S., 2005. Epstein-Barr virus infections: prospects for treatment. J. Antimicrob. Chemother., 56(2):277-281.

[12]Hemmingson, J.A., Falshaw, R., Furneaux, R.H., Thompson, K., 2006. Structure and antiviral activity of the galactofucan sulfates extracted from Undaria pinnatifida (Phaeophyta). J. Appl. Phycol., 18(2):185-193.

[13]Huleihel, M., Ishanu, V., Tal, J., Arad, S., 2001. Antiviral effect of red microalgal polysaccharides on Herpes simplex and Varicella zoster viruses. J. Appl. Phycol., 13(2):127-134.

[14]Klein, G., 1994. Epstein-Barr virus strategy in normal and neoplastic B cells. Cell, 77(6):791-793.

[15]Krenauer, A., Moll, A., Ponisch, W., Schmitz, N., Niedobitek, G., Niederwieser, D., Aigner, T., 2010. EBV-associated post-transplantation B-cell lymphoproliferative disorder following allogenic stem cell transplantation for acute lymphoblastic leukaemia: tumor regression after reduction of immunosuppression—a case report. Diagn. Pathol., 5(1):21.

[16]Kurth, J., Spieker, T., Wustrow, J., Strickler, G.J., Hansmann, L.M., Rajewsky, K., Küppers, R., 2000. EBV-infected B cells in infectious mononucleosis: viral strategies for spreading in the B cell compartment and establishing latency. Immunity, 13(4):485-495.

[17]Lai, P.J., Chu, W.L., Naidu, R., Khoo, A.S.B., Kok, Y.Y., Shar, M.M., Ling, S.N., Mak, J.W., Lim, K.C., Balraj, P., et al., 2008. Antiproliferative activity of microalgal extracts on nasopharyngeal carcinoma (NPC) cells. Malaysian J. Sci., 27(2):19-31.

[18]Lin, J.C., 2003. Mechanism of action of glycyrrhizic acid in inhibition of Epstein-Barr virus replication in vitro. Antiviral Res., 59(1):41-47.

[19]Lin, T.P., Chen, S.Y., Duh, P.D., Chang, L.K., Liu, Y.N., 2008. Inhibition of the Epstein-Barr virus lytic cycle by andrographolide. Biol. Pharm. Bull., 31(11):2018-2023.

[20]Lo, D.Y.M., Chan, L.Y.S., Lo, K.W., Leung, S.F., Zhang, J., Chan, A.T.C., Lee, J.C.K., Hjelm, N.M., Johnson, P.J., Huang, D.P., 1999. Quantitative analysis of cell-free Epstein-Barr virus DNA in plasma of patients with nasopharyngeal carcinoma. Cancer Res., 59:1188-1191.

[21]Mosmann, T., 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65(1-2):55-63.

[22]Ohigashi, H., Sakai, Y., Yamaguchi, K., Umezaki, I., Koshimizu, K., 1992. Possible anti-tumor promoting properties of marine algae and in vivo activity of Wakame seaweed extract. Biosci. Biotechnol. Biochem., 56(6):994-995.

[23]Phang, S.M., Chu, W.L., 1999. University of Malaya Algae Culture Collection (UMACC): Catalogue of Strains. Institute of Postgraduate Studies and Research, University of Malaya, Kuala Lumpur, Malaysia, p.77.

[24]Rechter, S., König, T., Auerochs, S., Thulke, S., Walter, H., Dörnenburg, H., Walter, C., Marschall, M., 2006. Antiviral activity of Arthrospira-derived spirulan-like substances. Antiviral Res., 72(3):197-206.

[25]Rezk, S., Weiss, L., 2007. Epstein-Barr virus-associated lymphoproliferative disorders. Human Pathol., 38(9):1293-1304.

[26]Rickinson, A.B., Kieff, E., 1996. Epstein-Barr Virus. In: Fields, B.N., Knipe, B.N., Howley, P.M. (Eds.), Fields Virology. Lippincott-Williams and Wilkins, Philadelphia, p.2397-2476.

[27]Rocha, F.D., Soares, A.R., Houghton, P.J., Pereira, R.C., Kaplan, M.A.C., Teixeira, V.L., 2007. Potential cytotoxic activity of some Brazilian seaweeds on human melanoma cells. Phytother. Res., 21(2):170-175.

[28]Schwarzmann, F., Jager, M., Horner, M., Prang, N., Wolf, H., 1998. Epstein-Barr viral gene expression in B-lymphocytes. Leuk. Lymphoma, 30(1-2):123-129.

[29]Takada, K., Shimizu, N., Tanabe-Tochikura, A., Kuroiwa, Y., 1995. Pathogenic role of Epstein-Barr virus in human cancer. Intervirology, 38(3-4):214-220.

[30]Walling, D.M., Flaitz, C.M., Nicholas, C.M., 2003. Epstein-Barr virus replication in oral hairy leukoplakia: response, persistence, and resistance to treatment with valacyclovir. J. Infect. Dis., 188(6):883-890.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE