CLC number: Q93; R511
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2011-04-06
Cited: 3
Clicked: 6708
Yih-Yih Kok, Wan-Loy Chu, Siew-Moi Phang, Shar Mariam Mohamed, Rakesh Naidu, Pey-Jiun Lai, Shui-Nyuk Ling, Joon-Wah Mak, Patricia Kim-Chooi Lim, Pauline Balraj, Alan Soo-Beng Khoo. Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells[J]. Journal of Zhejiang University Science B, 2011, 12(5): 335-345.
@article{title="Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells",
author="Yih-Yih Kok, Wan-Loy Chu, Siew-Moi Phang, Shar Mariam Mohamed, Rakesh Naidu, Pey-Jiun Lai, Shui-Nyuk Ling, Joon-Wah Mak, Patricia Kim-Chooi Lim, Pauline Balraj, Alan Soo-Beng Khoo",
journal="Journal of Zhejiang University Science B",
volume="12",
number="5",
pages="335-345",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000336"
}
%0 Journal Article
%T Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells
%A Yih-Yih Kok
%A Wan-Loy Chu
%A Siew-Moi Phang
%A Shar Mariam Mohamed
%A Rakesh Naidu
%A Pey-Jiun Lai
%A Shui-Nyuk Ling
%A Joon-Wah Mak
%A Patricia Kim-Chooi Lim
%A Pauline Balraj
%A Alan Soo-Beng Khoo
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 5
%P 335-345
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000336
TY - JOUR
T1 - Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells
A1 - Yih-Yih Kok
A1 - Wan-Loy Chu
A1 - Siew-Moi Phang
A1 - Shar Mariam Mohamed
A1 - Rakesh Naidu
A1 - Pey-Jiun Lai
A1 - Shui-Nyuk Ling
A1 - Joon-Wah Mak
A1 - Patricia Kim-Chooi Lim
A1 - Pauline Balraj
A1 - Alan Soo-Beng Khoo
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 5
SP - 335
EP - 345
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000336
Abstract: This study aimed to assess the inhibitory activities of methanol extracts from the microalgae Ankistrodesmus convolutus, Synechococcus elongatus, and Spirulina platensis against epstein-Barr virus (EBV) in three Burkitt’s lymphoma (BL) cell lines, namely Akata, B95-8, and P3HR-1. The antiviral activity was assessed by quantifying the cell-free EBV DNA using real-time polymerase chain reaction (PCR) technique. The methanol extracts from Ankistrodesmus convolutus and Synechococcus elongatus displayed low cytotoxicity and potent effect in reducing cell-free EBV DNA (EC50<0.01 µg/ml) with a high therapeutic index (>28000). After fractionation by column chromatography, the fraction from Synechococcus elongatus (SEF1) reduced the cell-free EBV DNA most effectively (EC50=2.9 µg/ml, therapeutic index>69). Upon further fractionation by high performance liquid chromatography (HPLC), the sub-fraction SEF1’a was most active in reducing the cell-free EBV DNA (EC50=1.38 µg/ml, therapeutic index>14.5). This study suggests that microalgae could be a potential source of antiviral compounds that can be used against EBV.
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