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CLC number: TS201.3

On-line Access: 2018-04-04

Received: 2017-01-20

Revision Accepted: 2017-05-15

Crosschecked: 2018-02-20

Cited: 0

Clicked: 1572

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Bin Qi

https://orcid.org/0000-0001-9708-5597

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.4 P.285-292

http://doi.org/10.1631/jzus.B1700040


Biosynthesis of (R)-2-hydroxy-3-phenylpropionic acid using whole recombinant Escherichia coli cells in an aqueous/n-octane biphasic system


Author(s):  Yi-bo Zhu, Yan Xu, Li-mei Wang, Bin Qi

Affiliation(s):  Key Laboratory of Food and Biotechnology of Suzhou, Changshu Institute of Technology, Changshu 215500, China; more

Corresponding email(s):   qibin65@126.com

Key Words:  (R)-2-hydroxy-3-phenylpropionic acid, Phenylpyruvate, Aqueous/n-octane biphasic system, Whole cell bioconversion, Recombinant Escherichia coli


Yi-bo Zhu, Yan Xu, Li-mei Wang, Bin Qi. Biosynthesis of (R)-2-hydroxy-3-phenylpropionic acid using whole recombinant Escherichia coli cells in an aqueous/n-octane biphasic system[J]. Journal of Zhejiang University Science B, 2018, 19(4): 285-292.

@article{title="Biosynthesis of (R)-2-hydroxy-3-phenylpropionic acid using whole recombinant Escherichia coli cells in an aqueous/n-octane biphasic system",
author="Yi-bo Zhu, Yan Xu, Li-mei Wang, Bin Qi",
journal="Journal of Zhejiang University Science B",
volume="19",
number="4",
pages="285-292",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700040"
}

%0 Journal Article
%T Biosynthesis of (R)-2-hydroxy-3-phenylpropionic acid using whole recombinant Escherichia coli cells in an aqueous/n-octane biphasic system
%A Yi-bo Zhu
%A Yan Xu
%A Li-mei Wang
%A Bin Qi
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 4
%P 285-292
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700040

TY - JOUR
T1 - Biosynthesis of (R)-2-hydroxy-3-phenylpropionic acid using whole recombinant Escherichia coli cells in an aqueous/n-octane biphasic system
A1 - Yi-bo Zhu
A1 - Yan Xu
A1 - Li-mei Wang
A1 - Bin Qi
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 4
SP - 285
EP - 292
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700040


Abstract: 
(R)-2-hydroxy-3-phenylpropionic acid (PLA) is an ideal antimicrobial compound with broad-spectrum activity against a wide range of Gram-positive bacteria, some Gram-negative bacteria, and fungi. We studied the bioconversion of phenylpyruvate (PPA) to PLA using whole recombinant Escherichia coli cells in a series of buffer/organic solvent systems. Octane was found to be the best organic solvent. The optimum volume ratio of the water phase to the n-octane phase, conversion temperature, substrate concentration, and cell concentration were 6:4, 40 °C, 12.5 g/L, and 30 g/L wet cells, respectively. Under the optimized conditions, the average PLA productivity in the aqueous/ n-octane system was 30.69% higher than that in the aqueous system, and 32.31 g/L PLA was obtained with the use of a stirred reactor (2-L scale). Taken together, our findings indicated that PLA biosynthesis was more efficient in an aqueous/n-octane biphasic system than in a monophasic aqueous system. The proposed biphasic system is an effective strategy for enhancing PLA yield and the biosynthesis of its analogues.

在水/正辛烷双相体系中利用重组大肠杆菌全细胞合成苯基乳酸的研究

目的:通过构建水/正辛烷双相体系用于苯基乳酸的合成,有效减轻产物抑制作用,改善细胞活力,增加苯基乳酸的产率及转化率.
创新点:在苯基乳酸的全细胞合成中,苯基乳酸的抑制作用导致微生物细胞活力降低,产率较低.本研究旨在降低产物抑制作用来增加苯基乳酸的产率及转化率.
方法:从极性不同的几种有机溶剂中筛选能够增加苯基乳酸产量的有机溶剂与水形成双相体系,在水/正辛烷双相体系中利用重组大肠杆菌全细胞转化法合成苯基乳酸,并对该体系转化过程进行优化.部分产物转移至正辛烷相中,减轻了产物抑制作用.
结论:本研究通过构建水/正辛烷双相体系全细胞合成苯基乳酸,优化了转化条件.正辛烷相的存在减轻了产物抑制作用.在最佳条件下,双相体系分批补料合成的苯基乳酸明显高于纯水相系统的产量.

关键词:苯基乳酸;苯丙酮酸;水/正辛烷双相体系;全细胞转化;重组大肠杆菌

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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