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CLC number: R711.75

On-line Access: 2012-11-05

Received: 2011-12-12

Revision Accepted: 2012-06-29

Crosschecked: 2012-09-14

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.11 P.894-903

http://doi.org/10.1631/jzus.B1100369


Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice


Author(s):  Qiong-yan Lin, Yi-feng Wang, Hui-nan Weng, Xiu-jie Sheng, Qing-ping Jiang, Zhi-ying Yang

Affiliation(s):  Department of Obstetrics and Gynecology, Key Laboratory for Major Obstetric Diseases of Guangdong Province, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China; more

Corresponding email(s):   wotaoqiwawa@163.com

Key Words:  Gonadotrophin-releasing hormone, Cisplatin, Ovarian cancer, Animal experiment


Qiong-yan Lin, Yi-feng Wang, Hui-nan Weng, Xiu-jie Sheng, Qing-ping Jiang, Zhi-ying Yang. Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice[J]. Journal of Zhejiang University Science B, 2012, 13(11): 894-903.

@article{title="Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice",
author="Qiong-yan Lin, Yi-feng Wang, Hui-nan Weng, Xiu-jie Sheng, Qing-ping Jiang, Zhi-ying Yang",
journal="Journal of Zhejiang University Science B",
volume="13",
number="11",
pages="894-903",
year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100369"
}

%0 Journal Article
%T Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice
%A Qiong-yan Lin
%A Yi-feng Wang
%A Hui-nan Weng
%A Xiu-jie Sheng
%A Qing-ping Jiang
%A Zhi-ying Yang
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 11
%P 894-903
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100369

TY - JOUR
T1 - Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice
A1 - Qiong-yan Lin
A1 - Yi-feng Wang
A1 - Hui-nan Weng
A1 - Xiu-jie Sheng
A1 - Qing-ping Jiang
A1 - Zhi-ying Yang
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 11
SP - 894
EP - 903
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100369


Abstract: 
Background and objective: Gonadotropin-releasing hormone (GnRH) plays an important role in the regulation of ovarian function and ovarian cancer cell growth. In this study, we determined whether administration of the GnRH agonist (GnRHa), triporelin, prior to cisplatin treatment affects cisplatin and/or prevents cisplatin-induced ovarian damage. Methods: nu/nu mice were injected with ovarian cancer OVCAR-3 cells intraperitoneally. After two weeks, the mice were treated with saline (control), cisplatin, GnRHa, or cisplatin plus GnRHa for four weeks. At the end of the experimental protocol, blood, tumor, ovary, and uterine tissues were resected for hematoxylin and eosin (H&E) staining, immunohistochemical analyses of Ki67, nuclear factor-κB (NF-κB), and caspase-3, transmission electron microscopy of apoptosis, or enzyme-linked immunosorbent assay (ELISA) analyses of anti-Mullerian hormone (AMH). Results: cisplatin treatment effectively inhibited tumor growth in mice treated with human ovarian cancer cells; however the treatment also induced considerable toxicity. Immunohistochemical analyses showed that Ki67 expression was reduced in cisplatin-treated mice compared to control (P<0.05), but there was no statistically significant differences between cisplatin-treated mice and cisplatin plus GnRHa-treated mice (P>0.05), while expressions of NF-κB and caspase-3 were reduced and induced, respectively, in cisplatin-treated mice and cisplatin plus GnRHa-treated mice. Apoptosis occurred in the GnRHa, cisplatin, and cisplatin plus GnRHa-treated mice, but not in control mice. Ovaries exposed to GnRHa in both GnRHa mice and cisplatin-treated mice (combination group) had significantly more primordial and growth follicles and serum levels of AMH than those in the control mice and cisplatin-treated mice (P<0.05). Conclusions: Administration of GnRHa to mice significantly decreased the extent of ovarian damage induced by cisplatin, but did not affect the anti-tumor activity of cisplatin.

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