Full Text:   <194>

Summary:  <66>

CLC number: R737.31

On-line Access: 2020-04-07

Received: 2019-07-20

Revision Accepted: 2019-11-15

Crosschecked: 2020-03-03

Cited: 0

Clicked: 283

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Lin Deng

https://orcid.org/0000-0002-3648-8066

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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.4 P.315-326

10.1631/jzus.B1900445


Cyclooxygenase-2 promotes ovarian cancer cell migration and cisplatin resistance via regulating epithelial mesenchymal transition


Author(s):  Lin Deng, Ding-qing Feng, Bin Ling

Affiliation(s):  China-Japan Friendship Hospital, Beijing 100029, China; more

Corresponding email(s):   lingbin.ling@vip.sina.com

Key Words:  Ovarian cancer (OC), Cyclooxygenase-2 (COX-2), Drug resistance, Migration, Epithelial-mesenchymal transition (EMT)


Lin Deng, Ding-qing Feng, Bin Ling. Cyclooxygenase-2 promotes ovarian cancer cell migration and cisplatin resistance via regulating epithelial mesenchymal transition[J]. Journal of Zhejiang University Science B, 2020, 21(4): 315-326.

@article{title="Cyclooxygenase-2 promotes ovarian cancer cell migration and cisplatin resistance via regulating epithelial mesenchymal transition",
author="Lin Deng, Ding-qing Feng, Bin Ling",
journal="Journal of Zhejiang University Science B",
volume="21",
number="4",
pages="315-326",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1900445"
}

%0 Journal Article
%T Cyclooxygenase-2 promotes ovarian cancer cell migration and cisplatin resistance via regulating epithelial mesenchymal transition
%A Lin Deng
%A Ding-qing Feng
%A Bin Ling
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 4
%P 315-326
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900445

TY - JOUR
T1 - Cyclooxygenase-2 promotes ovarian cancer cell migration and cisplatin resistance via regulating epithelial mesenchymal transition
A1 - Lin Deng
A1 - Ding-qing Feng
A1 - Bin Ling
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 4
SP - 315
EP - 326
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900445


Abstract: 
Objective: Drug-resistance and metastasis are major reasons for the high mortality of ovarian cancer (OC) patients. cyclooxygenase-2 (COX-2) plays a critical role in OC development. This study was designed to evaluate the effects of COX-2 on migration and cisplatin (cis-dichloro diammine platinum, CDDP) resistance of OC cells and explore its related mechanisms. Methods: Cell counting kit-8 (CCK-8) assay was used to detect the cytotoxicity effects of celecoxib (CXB) and CDDP on SKOV3 and ES2 cells. The effect of COX-2 on migration was evaluated via the healing test. Western blot and real-time quantitative polymerase chain reaction (qPCR) were used to analyze E-cadherin, vimentin, Snail, and Slug levels. Results: COX-2 promoted drug-resistance and cell migration. CXB inhibited these effects. The combination of CDDP and CXB increased tumor cell sensitivity, reduced the amount of CDDP required, and shortened treatment administration time. COX-2 upregulation increased the expression of Snail and Slug, resulting in E-cadherin expression downregulation and vimentin upregulation. Conclusions: COX-2 promotes cancer cell migration and CDDP resistance and may serve as a potential target for curing OC.

环氧合酶-2(COX-2)通过调控细胞上皮间质转化(EMT)促进卵巢癌细胞迁移及其耐药性

目的:研究环氧合酶-2(COX-2)对卵巢癌细胞迁移和耐药性的影响及其机制.
创新点:本研究发现COX-2对卵巢癌发生发展有一定的促进作用,可以通过上皮间质转化(EMT)途径促进卵巢癌细胞的迁移和顺铂(CDDP)耐药.其抑制剂塞来昔布(CXB)能起到协同抗癌的效果.
方法:CCK-8检测CXB和CDDP对SKOV3和ES2细胞的毒性作用.划痕实验评估COX-2对卵巢癌细胞迁移的作用.蛋白质免疫印迹(western blot)和聚合酶链式反应(PCR)检测EMT相关基因和蛋白的表达水平.
结论:COX-2可以通过EMT促进卵巢癌细胞迁移和CDDP耐药;CXB可以起到抑制作用,与CDDP协同抗癌.COX-2可以作为卵巢癌治疗的一个潜在靶点.

关键词:卵巢癌;环氧合酶-2(COX-2);耐药;迁移;上皮间质转化(EMT)

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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