CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2024-08-19
Cited: 0
Clicked: 1097
Junfen XU, Mengyan TU. Single-cell transcriptomics reveals tumor landscape in ovarian carcinosarcoma[J]. Journal of Zhejiang University Science B, 2024, 25(8): 686-699.
@article{title="Single-cell transcriptomics reveals tumor landscape in ovarian carcinosarcoma",
author="Junfen XU, Mengyan TU",
journal="Journal of Zhejiang University Science B",
volume="25",
number="8",
pages="686-699",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2300407"
}
%0 Journal Article
%T Single-cell transcriptomics reveals tumor landscape in ovarian carcinosarcoma
%A Junfen XU
%A Mengyan TU
%J Journal of Zhejiang University SCIENCE B
%V 25
%N 8
%P 686-699
%@ 1673-1581
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2300407
TY - JOUR
T1 - Single-cell transcriptomics reveals tumor landscape in ovarian carcinosarcoma
A1 - Junfen XU
A1 - Mengyan TU
J0 - Journal of Zhejiang University Science B
VL - 25
IS - 8
SP - 686
EP - 699
%@ 1673-1581
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2300407
Abstract: ObjectiveThe present study used single-cell RNA sequencing (scRNA-seq) to characterize the cellular composition of ovarian carcinosarcoma (OCS) and identify its molecular characteristics.
MethodsscRNA-seq was performed in resected primary OCS for an in-depth analysis of tumor cells and the tumor microenvironment. Immunohistochemistry staining was used for validation. The scRNA-seq data of OCS were compared with those of high-grade serous ovarian carcinoma (HGSOC) tumors and other OCS tumors.
ResultsBoth malignant epithelial and malignant mesenchymal cells were observed in the OCS patient of this study. We identified four epithelial cell subclusters with different biological roles. Among them, epithelial subcluster 4 presented high levels of breast cancer type 1 susceptibility protein homolog (BRCA1) and DNA topoisomerase 2-α (TOP2A) expression and was related to drug resistance and cell cycle. We analyzed the interaction between epithelial and mesenchymal cells and found that fibroblast growth factor (FGF) and pleiotrophin (PTN) signalings were the main pathways contributing to communication between these cells. Moreover, we compared the malignant epithelial and mesenchymal cells of this OCS tumor with our previous published HGSOC scRNA-seq data and OCS data. All the epithelial subclusters in the OCS tumor could be found in the HGSOC samples. Notably, the mesenchymal subcluster C14 exhibited specific expression patterns in the OCS tumor, characterized by elevated expression of cytochrome P450 family 24 subfamily A member 1 (CYP24A1), collagen type XXIII α1 chain (COL23A1), cholecystokinin (CCK), bone morphogenetic protein 7 (BMP7), PTN, Wnt inhibitory factor 1 (WIF1), and insulin-like growth factor 2 (IGF2). Moreover, this subcluster showed distinct characteristics when compared with both another previously published OCS tumor and normal ovarian tissue.
ConclusionsThis study provides the single-cell transcriptomics signature of human OCS, which constitutes a new resource for elucidating OCS diversity.
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