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On-line Access: 2022-04-11

Received: 2021-10-14

Revision Accepted: 2022-01-16

Crosschecked: 2022-04-19

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Shaohong LU


Xunhui ZHUO


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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.4 P.315-327


Toxoplasma gondii infection induces cell apoptosis via multiple pathways revealed by transcriptome analysis

Author(s):  Kaige DU, Fei LU, Chengzuo XIE, Haojie DING, Yu SHEN, Yafan GAO, Shaohong LU, Xunhui ZHUO

Affiliation(s):  School of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College, Hangzhou 310053, China; more

Corresponding email(s):   zhuoxh@zjams.com.cn, lsh@zjams.com.cn

Key Words:  Toxoplasma gondii, Transcriptome analysis, Apoptosis, Infection

Kaige DU, Fei LU, Chengzuo XIE, Haojie DING, Yu SHEN, Yafan GAO, Shaohong LU, Xunhui ZHUO. Toxoplasma gondii infection induces cell apoptosis via multiple pathways revealed by transcriptome analysis[J]. Journal of Zhejiang University Science B, 2022, 23(4): 315-327.

@article{title="Toxoplasma gondii infection induces cell apoptosis via multiple pathways revealed by transcriptome analysis",
author="Kaige DU, Fei LU, Chengzuo XIE, Haojie DING, Yu SHEN, Yafan GAO, Shaohong LU, Xunhui ZHUO",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Toxoplasma gondii infection induces cell apoptosis via multiple pathways revealed by transcriptome analysis
%A Kaige DU
%A Fei LU
%A Chengzuo XIE
%A Haojie DING
%A Yafan GAO
%A Shaohong LU
%A Xunhui ZHUO
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 4
%P 315-327
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100877

T1 - Toxoplasma gondii infection induces cell apoptosis via multiple pathways revealed by transcriptome analysis
A1 - Kaige DU
A1 - Fei LU
A1 - Chengzuo XIE
A1 - Haojie DING
A1 - Yu SHEN
A1 - Yafan GAO
A1 - Shaohong LU
A1 - Xunhui ZHUO
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 4
SP - 315
EP - 327
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100877

Toxoplasma gondii is a worldwide parasite that can infect almost all kinds of mammals and cause fatal toxoplasmosis in immunocompromised patients. apoptosis is one of the principal strategies of host cells to clear pathogens and maintain organismal homeostasis, but the mechanism of cell apoptosis induced by T. gondii remains obscure. To explore the apoptosis influenced by T. gondii, Vero cells infected or uninfected with the parasite were subjected to apoptosis detection and subsequent dual RNA sequencing (RNA-seq). Using high-throughput Illumina sequencing and bioinformatics analysis, we found that pro-apoptosis genes such as DNA damage-inducible transcript 3 (DDIT3), growth arrest and DNA damage-inducible α (GADD45A), caspase-3 (CASP3), and high-temperature requirement protease A2 (HtrA2) were upregulated, and anti-apoptosis genes such as poly(adenosine diphosphate (ADP)-ribose) polymerase family member 3 (PARP3), B-cell lymphoma 2 (Bcl-2), and baculoviral inhibitor of apoptosis protein (IAP) repeat containing 5 (BIRC5) were downregulated. Besides, tumor necrosis factor (TNF) receptor-associated factor 1 (TRAF1), TRAF2, TNF receptor superfamily member 10b (TNFRSF10b), disabled homolog 2 (DAB2)‍-interacting protein (DAB2IP), and inositol 1,4,5-trisphosphate receptor type 3 (ITPR3) were enriched in the upstream of TNF, TNF-related apoptosis-inducing ligand (TRAIL), and endoplasmic reticulum (ER) stress pathways, and TRAIL-receptor 2 (TRAIL-R2) was regarded as an important membrane receptor influenced by T. gondii that had not been previously considered. In conclusion, the T. gondii RH strain could promote and mediate apoptosis through multiple pathways mentioned above in Vero cells. Our findings improve the understanding of the T. gondii infection process through providing new insights into the related cellular apoptosis mechanisms.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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