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On-line Access: 2010-09-08

Received: 2010-03-09

Revision Accepted: 2010-06-07

Crosschecked: 2010-07-30

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.9 P.654-660

10.1631/jzus.B1000081


Cordycepin induces apoptosis by enhancing JNK and p38 kinase activity and increasing the protein expression of Bcl-2 pro-apoptotic molecules


Author(s):  Wei He, Mei-fang Zhang, Jun Ye, Ting-ting Jiang, Xu Fang, Ying Song

Affiliation(s):  The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China, Microbiology Institute of Zhejiang Province, Hangzhou 310011, China, College of Pharmacology, Zhejiang University of Technology, Hangzhou 310014, China

Corresponding email(s):   heweihz@126.com

Key Words:  Colorectal cancer, Cordycepin, Proliferation, Apoptosis, Bcl-2 molecules


Wei He, Mei-fang Zhang, Jun Ye, Ting-ting Jiang, Xu Fang, Ying Song. Cordycepin induces apoptosis by enhancing JNK and p38 kinase activity and increasing the protein expression of Bcl-2 pro-apoptotic molecules[J]. Journal of Zhejiang University Science B, 2010, 11(9): 654-660.

@article{title="Cordycepin induces apoptosis by enhancing JNK and p38 kinase activity and increasing the protein expression of Bcl-2 pro-apoptotic molecules",
author="Wei He, Mei-fang Zhang, Jun Ye, Ting-ting Jiang, Xu Fang, Ying Song",
journal="Journal of Zhejiang University Science B",
volume="11",
number="9",
pages="654-660",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000081"
}

%0 Journal Article
%T Cordycepin induces apoptosis by enhancing JNK and p38 kinase activity and increasing the protein expression of Bcl-2 pro-apoptotic molecules
%A Wei He
%A Mei-fang Zhang
%A Jun Ye
%A Ting-ting Jiang
%A Xu Fang
%A Ying Song
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 9
%P 654-660
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000081

TY - JOUR
T1 - Cordycepin induces apoptosis by enhancing JNK and p38 kinase activity and increasing the protein expression of Bcl-2 pro-apoptotic molecules
A1 - Wei He
A1 - Mei-fang Zhang
A1 - Jun Ye
A1 - Ting-ting Jiang
A1 - Xu Fang
A1 - Ying Song
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 9
SP - 654
EP - 660
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000081


Abstract: 
Objective: To explore the molecular mechanism by which cordycepin inhibits cell proliferation and induces apoptosis of human colorectal cancer cells. Methods: Cell counting and MTS (3-(4,5-dimethylthiazol-2-yl) -5-(3-carboxymethoxyphenyl)-2- (4-sulfopheny)-2H-tetrazolium, inner salt) method were used to monitor the effects of cordycepin on cell proliferation. Flow cytometry (FCM) was used to analyze the effects of cordycepin on the cell cycle progress. Annexin V-fluorescein isothiocyanate (FITC) analysis was used to detect apoptosis at a very early stage. Caspase-Glo was used to determine caspase activity and Western blot was used to measure protein expression levels of c-Jun N-terminal kinase (JNK), p38, and Bcl-2 pro-apoptosis family. Results: The numbers of viable SW480 and SW620 cells and the proliferation of these cells were significantly reduced with increases in cordycepin concentration (P<0.01). The cell cycle progression of SW480 and SW620 was arrested at the G0/G1 phase by the addition of cordycepin, and apoptosis rates of cordycepin treatments were increased compared with the control group. cordycepin-treated cells showed phosphatidylserine valgus, suggesting the existence of early apoptosis. Caspase-3/7 and -9 activity significantly increased and the protein expression levels of JNK, p38, and Bax, Bid, Bim, and Puma from Bcl-2 pro-apoptosis molecules also increased after the treatment with cordycepin. Conclusions: cordycepin can inhibit SW480 and SW620 cell proliferation and induce apoptosis. apoptosis might be induced by enhancing JNK and p38 kinase activity and increasing the protein expression of Bcl-2 pro-apoptotic molecules.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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