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Received: 2008-08-03

Revision Accepted: 2008-10-22

Crosschecked: 2009-01-05

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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.2 P.93~102


Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells

Author(s):  Jin-bo WANG, Li-li QI, Shui-di ZHENG, Tian-xing WU

Affiliation(s):  Department of Chemistry, College of Science, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   wutx@nit.zju.edu.cn

Key Words:  Curcumin, Apoptosis, Mitochondrial pathway, HT-29 cells

Jin-bo WANG, Li-li QI, Shui-di ZHENG, Tian-xing WU. Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells[J]. Journal of Zhejiang University Science B, 2009, 10(2): 93~102.

@article{title="Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells",
author="Jin-bo WANG, Li-li QI, Shui-di ZHENG, Tian-xing WU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells
%A Jin-bo WANG
%A Li-li QI
%A Shui-di ZHENG
%A Tian-xing WU
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 2
%P 93~102
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820238

T1 - Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells
A1 - Jin-bo WANG
A1 - Li-li QI
A1 - Shui-di ZHENG
A1 - Tian-xing WU
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 2
SP - 93
EP - 102
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820238

Objective: To investigate the effects of curcumin on release of cytochrome c and expressions of Bcl-2, Bax, Bad, Bcl-xL, caspase-3, poly ADP-ribose polymerase (PARP), and survivin of HT-29 cells. Methods: HT-29 cells were treated with curcumin (0~80 μmol/L) for 24 h. The release of cytochrome c from the mitochondria and the apoptosis-related proteins Bax, Bcl-2, Bcl-xL, Bad, caspase-3, PARP, and survivin were determined by Western blot analysis and their mRNA expressions by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: curcumin significantly induced the growth inhibition and apoptosis of HT-29 cells. A decrease in expressions of Bcl-2, Bcl-xL and survivin was observed after exposure to 10~80 μmol/L curcumin, while the levels of Bax and Bad increased in the curcumin-treated cells. curcumin also induced the release of cytochrome c, the activation of caspase-3, and the cleavage of PARP in a dose-dependent manner. Conclusion: These data suggest that curcumin induced the HT-29 cell apoptosis possibly via the mitochondria-mediated pathway.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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