Full Text:   <184>

Summary:  <72>

CLC number: R285.5

On-line Access: 2020-06-01

Received: 2019-08-29

Revision Accepted: 2020-01-17

Crosschecked: 2020-05-28

Cited: 0

Clicked: 491

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.6 P.485-494


Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing

Author(s):  Wei-Hua He, Xiao-Hu Zhai, Xiu-Jun Duan, He-Shuang Di

Affiliation(s):  Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China

Corresponding email(s):   zhaixiaohu010@163.com

Key Words:  Resveratrol (RSV), Boar semen freezing, Antioxidant, Mitochondrial function, Apoptotic pathway

Wei-Hua He, Xiao-Hu Zhai, Xiu-Jun Duan, He-Shuang Di. Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing[J]. Journal of Zhejiang University Science B, 2020, 21(6): 485-494.

@article{title="Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing",
author="Wei-Hua He, Xiao-Hu Zhai, Xiu-Jun Duan, He-Shuang Di",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing
%A Wei-Hua He
%A Xiao-Hu Zhai
%A Xiu-Jun Duan
%A He-Shuang Di
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 6
%P 485-494
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900520

T1 - Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing
A1 - Wei-Hua He
A1 - Xiao-Hu Zhai
A1 - Xiu-Jun Duan
A1 - He-Shuang Di
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 6
SP - 485
EP - 494
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900520

Resveratrol (3,5,4'-trihydroxystilbene, RSV) has been widely used in mammalian cells, but whether it can be used during freezing boar semen is still unknown. The effects of RSV treatment during boar semen freezing on its anti-freezing ability, apoptosis, and possible apoptotic pathways were observed in this study. Sperm motility, mitochondrial membrane potential (ΔΨm), adenosine triphosphate (ATP) content, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL)-positive apoptotic state, and messenger RNA (mRNA) expression levels of apoptotic genes involved in different apoptotic pathways after freezing with or without RSV treatment were tested. The results showed that: (1) Compared with fresh sperm, the motility, normal acrosome rate, and plasma membrane integrity rate of frozen boar sperm decreased significantly (P<0.05), and RSV did not significantly increase the sperm motility (0.44 vs. 0.40, P>0.05), but it did significantly improve the normal acrosome rate (57.65% vs. 47.00%, P<0.05) and plasma membrane integrity rate (46.67% vs. 38.85%, P<0.05). (2) After freezing, most boar sperm showed low mitochondrial ΔΨm. RSV treatment could increase the rate of high mitochondrial ΔΨm of boar sperm. (3) RSV treatment significantly decreased reactive oxygen species (ROS) levels (58.65% vs. 88.41%, P<0.05) and increased the ATP content (0.49 μmol/L vs. 0.25 μmol/L, P<0.05) of boar sperm during freezing. (4) The apoptotic rate of the freezing group (80.41%) with TUNEL detection increased significantly compared to the fresh group (9.70%, P<0.05), and RSV treatment greatly decreased the apoptotic rate (68.32%, P<0.05). (5) Real-time polymerase chain reaction (RT-PCR) showed that not only the genes from the death receptor-mediated apoptotic pathway (tumor necrosis factor-α (TNF-α), Fas ligand (FasL), and Caspase-8), but also the genes from the mitochondria-mediated apoptotic pathway (manganese superoxide dismutase (MnSOD), B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and Caspase-9) were both significantly changed after freezing. RSV treatment during freezing greatly changed their expression levels. Although RSV treatment during boar semen freezing did not significantly increase motility after thawing, it still played an efficient antioxidant role, which could enhance the mitochondrial function and decrease the apoptotic level induced by both the death receptor- and mitochondria-mediated apoptotic pathways.


方法:在猪精液的冷冻和解冻过程中添加1 mmol/L RSV,解冻后检测精子活力、线粒体膜电位、腺苷三磷酸(ATP)含量、凋亡水平和凋亡通路中相关基因的表达情况.
结论:(1)与鲜精相比,冷冻精液的活力、顶体完整性和质膜完整性均显著降低,冷冻前后RSV处理未能显著提高精子活力(0.44 vs. 0.40,P>0.05),但能显著提高顶体完整性(57.65% vs. 47.00%,P<0.05)和质膜完整性(46.67% vs. 38.85%,P<0.05).(2)解冻后精子线粒体膜电位显著下降,RSV的添加能提高精子膜电位水平. (3)冷冻解冻过程中添加RSV能显著降低精子的活性氧(ROS)水平(58.65% vs. 88.41%,P<0.05),增加精子的ATP含量(0.49 µmol/L vs. 0.25 µmol/L,P<0.05).(4)TUNEL凋亡检测后,冷冻精子的凋亡率(80.41%)与鲜精组(9.70%)相比显著增加(P<0.05),RSV处理能显著降低冻精的凋亡比例(68.32%,P<0.05).(5)实时荧光定量聚合酶链反应(qRT-PCR)的结果显示,猪精液冷冻后,无论是死亡受体介导凋亡途径中的相关基因(肿瘤坏死因子α(TNF-α)、TNF受体超族配体(FasL)和半胱氨酸的天冬氨酸蛋白水解酶8(Caspase-8)),还是线粒体介导凋亡途径中的相关基因(锰超氧化物歧化酶(MnSOD)、B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关X蛋白质(Bax)和半胱氨酸的天冬氨酸蛋白水解酶9(Caspase-9)),均产生明显变化,RSV添加亦能显著改变其表达水平.综上所述,猪精液冷冻解冻过程中RSV添加虽未能显著提高精子活力,但仍表现为抗氧化保护效果,体现在改善了线粒体功能,并通过改变死亡受体和线粒体介导的凋亡途径中相关基因的表达水平,降低了冻后精子的细胞凋亡.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Aitken RJ, Koppers AJ, 2011. Apoptosis and DNA damage in human spermatozoa. Asian J Androl, 13(1):36-42.

[2]Awda BJ, Mackenzie-Bell M, Buhr MM, 2009. Reactive oxygen species and boar sperm function. Biol Reprod, 81(3):553-561.

[3]Branco CS, Garcez ME, Pasqualotto FF, et al., 2010. Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen. Cryobiology, 60(2):235-237.

[4]Bucak MN, Ataman MB, Başpınar N, et al., 2015. Lycopene and resveratrol improve post-thaw bull sperm parameters: sperm motility, mitochondrial activity and DNA integrity. Andrologia, 47(5):545-552.

[5]Collodel G, Federico MG, Geminiani M, et al., 2011. Effect of trans-resveratrol on induced oxidative stress in human sperm and in rat germinal cells. Reprod Toxicol, 31(2):239-246.

[6]Dai JJ, Wu CF, Zhang DF, et al., 2009. Some factors affecting freezing of boar semen in 5 mL maxi-straws. Asian Australas J Anim Sci, 22(4):507-515.

[7]Dai JJ, Wu CF, Muneri CW, et al., 2015. Changes in mitochondrial function in porcine vitrified MII-stage oocytes and their impacts on apoptosis and developmental ability. Cryobiology, 71(2):291-298.

[8]Dai JJ, Niu YF, Wu CF, et al., 2016. Both death receptor and mitochondria mediated apoptotic pathways participated the occurrence of apoptosis in porcine vitrified MII stage oocytes. Cryo Letters, 37(2):129-136.

[9]de Oliveira MR, Nabavi SF, Manayi A, et al., 2016. Resveratrol and the mitochondria: from triggering the intrinsic apoptotic pathway to inducing mitochondrial biogenesis, a mechanistic view. Biochim Biophys Acta Gen Subj, 1860(4):727-745.

[10]Gadea J, Sellés E, Marco MA, et al., 2004. Decrease in glutathione content in boar sperm after cryopreservation: effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology, 62(3-4):690-701.

[11]Garcez ME, dos Santos Branco C, Lara LV, et al., 2010. Effects of resveratrol supplementation on cryopreservation medium of human semen. Fertil Steril, 94(6):2118-2121.

[12]Green DR, Reed JC, 1998. Mitochondria and apoptosis. Science, 281(5381):1309-1312.

[13]Gürler H, Malama E, Heppelmann M, et al., 2016. Effects of cryopreservation on sperm viability, synthesis of reactive oxygen species, and DNA damage of bovine sperm. Theriogenology, 86(2):562-571.

[14]Hsuuw YD, Chan WH, Yu JS, 2013. Ochratoxin a inhibits mouse embryonic development by activating a mitochondrion-dependent apoptotic signaling pathway. Int J Mol Sci, 14(1):935-953.

[15]Jeulin C, Soufir JC, Weber P, et al., 1989. Catalase activity in human spermatozoa and seminal plasma. Gamete Res, 24(2):185-196.

[16]Kefer JC, Agarwal A, Sabanegh E, 2009. Role of antioxidants in the treatment of male infertility. Int J Urol, 16(5):449-457.

[17]Li ZL, Lin QL, Liu RJ, et al., 2010. Protective effects of ascorbate and catalase on human spermatozoa during cryopreservation. J Androl, 31(5):437-444.

[18]Luño V, Gil L, Olaciregui M, et al., 2014. Rosmarinic acid improves function and in vitro fertilising ability of boar sperm after cryopreservation. Cryobiology, 69(1):157-162.

[19]Martinez-Soto JC, de DiosHourcade J, Gutiérrez-Adán A, et al., 2010. Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa. Asian J Androl, 12(3):431-441.

[20]Mukherjee A, Malik H, Saha AP, et al., 2014. Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression. J Assist Reprod Genet, 31(2):229-239.

[21]Ou XH, Li S, Wang ZB, et al., 2012. Maternal insulin resistance causes oxidative stress and mitochondrial dysfunction in mouse oocytes. Hum Reprod, 27(7):2130-2145.

[22]Pasqualotto FF, Gupta S, Borges E Jr, et al., 2006. Impact of agriculture on semen quality and oxidative stress amongst infertile couples. Fertil Steril, 86(3):S445-S446.

[23]Perchec G, Jeulin C, Cosson J, et al., 1995. Relationship between sperm ATP content and motility of carp spermatozoa. J Cell Sci, 108(2):747-753.

[24]Qiu Y, Wang LG, Jia YF, et al., 2011. Effects of the crude extract of Polygala tenuifolia Willd on human sperm in vitro. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 12(6):448-454.

[25]Schäfer S, Holzmann A, 2000. The use of transmigration and Spermac™ stain to evaluate epididymal cat spermatozoa. Anim Reprod Sci, 59(3-4):201-211.

[26]Scott FL, Stec B, Pop C, et al., 2009. The Fas-FADD death domain complex structure unravels signalling by receptor clustering. Nature, 457(7232):1019-1022.

[27]Shimizu K, Miyagi S, Miyazawa K, et al., 2016. Resveratrol prevents warm ischemia-reperfusion injury in liver grafts from non-heart-beating donor rats. Transplan Proc, 48(4):1221-1225.

[28]Silva ECB, Cajueiro JFP, Silva SV, et al., 2012. Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm. Theriogenology, 77(8):1722-1726.

[29]Tao ZH, Li C, Xu XF, et al., 2019. Scavenging activity and mechanism study of ferulic acid against reactive carbonyl species acrolein. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 20(11):868-876.

[30]Toni LS, Garcia AM, Jeffrey DA, et al., 2018. Optimization of phenol-chloroform RNA extraction. MethodsX, 5:599-608.

[31]Trzcińska M, Bryła M, Gajda B, et al., 2015. Fertility of boar semen cryopreserved in extender supplemented with butylated hydroxytoluene. Theriogenology, 83(3):307-313.

[32]Zeng CJ, Tang KY, He L, et al., 2014. Effects of glycerol on apoptotic signaling pathways during boar spermatozoa cryopreservation. Cryobiology, 68(3):395-404.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE