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Received: 2009-05-25

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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.8 P.619~624


Protective paracrine effect of mesenchymal stem cells on cardiomyocytes

Author(s):  Mei-xiang XIANG, Ai-na HE, Jian-an WANG, Chun GUI

Affiliation(s):  Department of Cardiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China

Corresponding email(s):   wang_jian_an@tom.com

Key Words:  Mesenchymal stem cell (MSC), Apoptosis, Mitochondrial transmembrane potential, Hypoxia/reoxygenation (H/R)

Mei-xiang XIANG, Ai-na HE, Jian-an WANG, Chun GUI. Protective paracrine effect of mesenchymal stem cells on cardiomyocytes[J]. Journal of Zhejiang University Science B, 2009, 10(8): 619~624.

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author="Mei-xiang XIANG, Ai-na HE, Jian-an WANG, Chun GUI",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Protective paracrine effect of mesenchymal stem cells on cardiomyocytes
%A Mei-xiang XIANG
%A Ai-na HE
%A Jian-an WANG
%A Chun GUI
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 8
%P 619~624
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920153

T1 - Protective paracrine effect of mesenchymal stem cells on cardiomyocytes
A1 - Mei-xiang XIANG
A1 - Ai-na HE
A1 - Jian-an WANG
A1 - Chun GUI
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 8
SP - 619
EP - 624
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920153

Objective: The aim of this study was to test the protective effect of mesenchymal stem cells (MSCs) on cardiomyocytes in vitro and to investigate the anti-apoptotic signaling pathway. Methods: MSCs from Sprague-Dawley (SD) rats were separated and cultured. MSC medium was collected from MSCs cultured in serum-free Dulbecco’s modified eagle medium (DMEM) under hypoxia. Cultured cardiomyocytes from neonatal SD rats were exposed to hypoxia/reoxygenation (H/R) and treated with MSC medium. The apoptotic cardiomyocytes were stained with Annexin-V-fluorescein isothiocyanate (FITC), Hoechst 33342 and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). The mitochondrial transmembrane potential of cardiomyocytes was assessed using a fluorescence microscope. The expression of Bcl-2, Bax, cytochrome C, apoptosis-induced factor (AIF), and caspase-3 was tested by Western blot analysis. Results: Our data demonstrated that MSC medium reduced H/R-induced cardiomyocyte apoptosis, increased the Bcl-2/Bax ratio, and reduced the release of cytochrome C and AIF from mitochondria into the cytosol. Conclusion: MSCs protected the cardiomyocytes from H/R-induced apoptosis through a mitochondrial pathway in a paracrine manner.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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