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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.1 P.18-27

http://doi.org/10.1631/jzus.B1000074


Immunomodulative effects of mesenchymal stem cells derived from human embryonic stem cells in vivo and in vitro


Author(s):  Zhou Tan, Zhong-yuan Su, Rong-rong Wu, Bin Gu, Yu-kan Liu, Xiao-li Zhao, Ming Zhang

Affiliation(s):  Institute of Cell Biology and Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China, Institute of Developmental and Regenerative Biology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China

Corresponding email(s):   zhangming_ls@zju.edu.cn

Key Words:  Human embryonic stem cells, Mesenchymal stem cells, Differentiation, Immunomodulative effects


Zhou Tan, Zhong-yuan Su, Rong-rong Wu, Bin Gu, Yu-kan Liu, Xiao-li Zhao, Ming Zhang. Immunomodulative effects of mesenchymal stem cells derived from human embryonic stem cells in vivo and in vitro[J]. Journal of Zhejiang University Science B, 2011, 12(1): 18-27.

@article{title="Immunomodulative effects of mesenchymal stem cells derived from human embryonic stem cells in vivo and in vitro",
author="Zhou Tan, Zhong-yuan Su, Rong-rong Wu, Bin Gu, Yu-kan Liu, Xiao-li Zhao, Ming Zhang",
journal="Journal of Zhejiang University Science B",
volume="12",
number="1",
pages="18-27",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000074"
}

%0 Journal Article
%T Immunomodulative effects of mesenchymal stem cells derived from human embryonic stem cells in vivo and in vitro
%A Zhou Tan
%A Zhong-yuan Su
%A Rong-rong Wu
%A Bin Gu
%A Yu-kan Liu
%A Xiao-li Zhao
%A Ming Zhang
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 1
%P 18-27
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000074

TY - JOUR
T1 - Immunomodulative effects of mesenchymal stem cells derived from human embryonic stem cells in vivo and in vitro
A1 - Zhou Tan
A1 - Zhong-yuan Su
A1 - Rong-rong Wu
A1 - Bin Gu
A1 - Yu-kan Liu
A1 - Xiao-li Zhao
A1 - Ming Zhang
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 1
SP - 18
EP - 27
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000074


Abstract: 
Objective: human embryonic stem cells (hESCs) have recently been reported as an unlimited source of mesenchymal stem cells (MSCs). The present study not only provides an identical and clinically compliant MSC source derived from hESCs (hESC-MSCs), but also describes the immunomodulative effects of hESC-MSCs in vitro and in vivo for a carbon tetrachloride (CCl4)-induced liver inflammation model. Methods: Undifferentiated hESCs were treated with Rho-associated kinase (ROCK) inhibitor and induced to fibroblast-looking cells. These cells were tested for their surface markers and multilineage differentiation capability. Further more, we analyzed their immune characteristics by mixed lymphocyte reactions (MLRs) and animal experiments. Results: hESC-MSCs show a homogenous fibroblastic morphology that resembles bone marrow-derived MSCs (BM-MSCs). The cell markers and differentiation potential of hESC-MSCs are also similar to those of BM-MSCs. Unlike their original cells, hESC-MSCs possess poor immunogenicity and can survive and be engrafted into a xenogenic immunocompetent environment. Conclusions: The hESC-MSCs demonstrate strong inhibitory effects on lymphocyte proliferation in vitro and anti-inflammatory infiltration properties in vivo. This study offers information essential to the applications of hESC-MSC-based therapies and evidence for the therapeutic mechanisms of action.

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