Full Text:   <1640>

CLC number: R239.28

On-line Access: 

Received: 2002-12-09

Revision Accepted: 2003-04-16

Crosschecked: 0000-00-00

Cited: 9

Clicked: 3393

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.2 P.157~163

http://doi.org/10.1631/jzus.2004.0157


Ex vivo expansions and transplantations of mouse bone marrow-derived hematopoietic stem/progenitor cells


Author(s):  WANG Jin-fu, WU Yi-fan, HARRINTONG Jenny, McNIECE Ian K.

Affiliation(s):  College of Life Sciences, Zhejiang University, Hangzhou 310012, China; more

Corresponding email(s):   wjflu@mail.hz.zj.cn

Key Words:  Hematopoietic stem cells, Mesenchymal stem cells, Expansion, Transplantation


Share this article to: More

WANG Jin-fu, WU Yi-fan, HARRINTONG Jenny, McNIECE Ian K.. Ex vivo expansions and transplantations of mouse bone marrow-derived hematopoietic stem/progenitor cells[J]. Journal of Zhejiang University Science A, 2004, 5(2): 157~163.

@article{title="Ex vivo expansions and transplantations of mouse bone marrow-derived hematopoietic stem/progenitor cells",
author="WANG Jin-fu, WU Yi-fan, HARRINTONG Jenny, McNIECE Ian K.",
journal="Journal of Zhejiang University Science A",
volume="5",
number="2",
pages="157~163",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0157"
}

%0 Journal Article
%T Ex vivo expansions and transplantations of mouse bone marrow-derived hematopoietic stem/progenitor cells
%A WANG Jin-fu
%A WU Yi-fan
%A HARRINTONG Jenny
%A McNIECE Ian K.
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 2
%P 157~163
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0157

TY - JOUR
T1 - Ex vivo expansions and transplantations of mouse bone marrow-derived hematopoietic stem/progenitor cells
A1 - WANG Jin-fu
A1 - WU Yi-fan
A1 - HARRINTONG Jenny
A1 - McNIECE Ian K.
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 2
SP - 157
EP - 163
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.0157


Abstract: 
To examine the effects of co-culture with bone marrow mesenchymal stem cells on expansion of hematopoietic stem/progenitor cells and the capacities of rapid neutrophil engraftment and hematopoietic reconstitution of the expanded cells, we expanded mononuclear cells (MNCs) and CD34+/c-kit+ cells from mouse bone marrow and transplanted the expanded cells into the irradiated mice. MNCs were isolated from mouse bone marrow and CD34+/c-kit+ cells were selected from MNCs by using MoFlo Cell Sorter. MNCs and CD34+/c-kit+ cells were co-cultured with mouse bone marrow-derived mesenchymal stem cells (MSCs) under a two-step expansion. The expanded cells were then transplanted into sublethally irradiated BDF1 mice. Results showed that the co-culture with MSCs resulted in expansions of median total nucleated cells, CD34+ cells, GM-CFC and HPP-CFC respectively by 10.8-, 4.8-, 65.9- and 38.8-fold for the mononuclear cell culture, and respectively by 76.1-, 2.9-, 71.7- and 51.8-fold for the CD34+/c-kit+ cell culture. The expanded cells could rapidly engraft in the sublethally irradiated mice and reconstitute their hematopoiesis. Co-cultures with MSCs in conjunction with two-step expansion increased expansions of total nucleated cells, GM-CFC and HPP-CFC, which led us to conclude MSCs may create favorable environment for expansions of hematopoietic stem/progenitor cells. The availability of increased numbers of expanded cells by the co-culture with MSCs may result in more rapid engraftment of neutrophils following infusion to transplant recipients.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Faucher, C., Le Corroller, A.G., Blaise, D., Novakovitch, G., Manonni, P., 1994. Comparison of GCSF-promed peripheral blood progenitor cells and bone marrow auto transplantation: clinical assessment and cost-effectiveness.Bone Marrow Transplant,14:895-901.

[2] Glaspy, J.A., Shpall, E.J., LeMaistre, C.F., Briddell, R.A., Menchaca, D.M., Turner, S.A., Lill, M., Chap, L., Jones, R., Wiers, M.D., Sheridan, W.P., McNiece, I.K., 1997. Peripheral blood progenitor cell mobilization using stem cell factor in combination with filgrastim in breast cancer patients.Blood,90:2939-2951.

[3] Gluckman, E., Rocha, V., Boyer-Chammard, A., Locatelli, F., Arcese, W., Pasquini, R., Ortega, J., Souillet, G., Ferreira, E., Laporte, J.P., Fernandez, M., Chastang, C., 1997. Outcome of cord-blood transplantation from related and unrealated donors.N Engl J Med,337:373-381.

[4] Hartmann, O., Le Corroller, A.G., Blaise, D., Michon, J., Philip, I., Norol, F., Janvier, M., Pico, J.L., Baranzelli, M.C., Rubie, H., Coze, C., Pinna, A., Meresse, V., Benhamou, E., 1997. Peripheral blood stem cell and bone marrow transplantation for solid tumors and lymphomas: hematologic recovery and costs.Ann Intern Med,126:600-607.

[5] McNiece, I., Jones, R., Bearman, S.I., Cagnoni, P., Nieto, Y., Murphy, J., Shpall, E.J., 1999. Rapid engraftment of neutrophils using ex vivo expanded PBPC correlates with total nucleated cells and not CD34+cells.Blood,94(sup.):554a.

[6] McNiece, I., Jones, R., Bearman, S.I., Cagnoni, P., Nieto, Y., Franklin, W., Ryder, J., Steele, A., Stoltz, J., Russell, P., McDermitt, J., Hogan, C., Murphy, J., Shpall, E.J., 2000a. Ex vivo expanded peripheral blood progenitor cells provide rapid neutrophil recovery after high-dose chemotherapy in patients with breast cancer.Blood,96:3001-3007.

[7] McNiece, I.K., Kubegov, D., Kerzic, P., Shpall, E.J., Gross, S., 2000b. Increased expansion and differentiation of cord blood products using a two-step expansion culture.Experi Hematol,28:1181-1186.

[8] Paquette, R.L., Dergham, S.T., Karpf, E., Wang, H.J., Slamon, D.J., Souza, L., Glaspy, J.A., 2000. Ex vivo expanded unselected peripheral blood progenitor cells reduce post transplant neutropenia, thrombocytopenia, and anemia in patients with breast cancer.Blood,96:2385-2390.

[9] Paquette, R.L., Dergham, S.T., Karpf, E., Wang, H.J., Slamon, D.J., Souza, L., Glaspy, J.A., 2002. Culture conditions affect the ability of ex vivo expanded peripheral blood progenitor cells to accelerate hematopoietic recovery.Experi Hematol,30:374-380.

[10] Peters, W.P., Shpall, E.J., Jones, R.B., Olsen, G.A., Bast, R.C., Gockerman, J.P., Moore, J.O., 1988. High-dose combination alkylating agents with bone marrow support as an initial treatment for metastatic breast cancer.J Clin Oncol,6:1368-1376.

[11] Reiffers, J., Cailliot, C., Dazey, B., Attal, M., Caraux, J., Boiron, J.M., 1999. Abrogation of post-myeloablative chemotherapy neutropenia by ex vivo expanded auto-logous CD34-postive cells.Lancet,354:1092-1093.

[12] Schmitz, N., Linch, D.C., Dreger, P., Goldstone, A.H., Boogaerts, M.A., Ferrant, A., Demuynck, H.M., Link, H., Zander, A., Barge, A., 1996. Randomised trial of filgrastim-mobilised peripheral blood progenitor cell transplantation versus autologous bone-marrow transplantation in lymphoma patients.Lancet,347:353-357.

[13] Sheridan, W., Begley, C., Juttner, C., Szer, J., To, L.B., Maher, D., McGrath, K.M., Morstyn, G., Fox, R.M., 1992. Effect of peripheral blood progenitor cells mobilized by figrastim (G-CSF) on platelet recovery after high-dose chemotherapy.Lancet,339:640-644.

[14] Shpall, E.J., Quinones, R., Jones, R., Zeng, C., Baron, A.E., Jones, R.B., Bearman, S.I., Nieto, Y., Freed, B., Madinger, N., Hogan, C.J., Slat-Vasquez, V., Russell, P., Blunk, B., Schissel, D., Hild, E., Malcolm, J., Ward, W., McNiece, I.K., 1999. Transplantation of adult and pediatric cancer patients with cord blood progenitors expanded ex vivo.Blood,94(sup.):712a.

[15] To, L.B., Roberts, M.M., Haylock, D.N., Dyson, P.G., Branford, A.L., Thorp, D., Ho, J.Q., Dart, G.W., Horvath, N., Davy, M.L., 1992. Comparison of haematological recovery times and supportive care requirements of autologous recovery phase peripheral blood stem cell transplants, autologous bone marrow transplants and allogeneic bone marrow transplants.Bone Marrow Transplant,9:277-284.

[16] Wang, J.F., Wang, L.J., Harrintong, J., McNiece, I.K., 2003. Effects of bone marrow-derived mesenchymal stem cell layer on expansion and differentiation of hematopoietic stem cells from cord blood.J Zhejiang University(Sci. edtion),30:93-97(in Chinese).

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE