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Received: 2004-09-26

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.4 P.242~248


Human bone marrow-derived mesenchymal stem cells transplanted into damaged rabbit heart to improve heart function

Author(s):  WANG Jian-an, FAN You-qi, LI Chang-ling, HE Hong, SUN Yong, LV Bin-jian

Affiliation(s):  Department of Cardiology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China

Corresponding email(s):   fanyouqi@tom.com

Key Words:  Bone marrow-derived mesenchymal stem cells, Transplantation, Myocardial infarction (MI)

WANG Jian-an, FAN You-qi, LI Chang-ling, HE Hong, SUN Yong, LV Bin-jian. Human bone marrow-derived mesenchymal stem cells transplanted into damaged rabbit heart to improve heart function[J]. Journal of Zhejiang University Science B, 2005, 6(4): 242~248.

@article{title="Human bone marrow-derived mesenchymal stem cells transplanted into damaged rabbit heart to improve heart function",
author="WANG Jian-an, FAN You-qi, LI Chang-ling, HE Hong, SUN Yong, LV Bin-jian",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Human bone marrow-derived mesenchymal stem cells transplanted into damaged rabbit heart to improve heart function
%A WANG Jian-an
%A FAN You-qi
%A LI Chang-ling
%A HE Hong
%A SUN Yong
%A LV Bin-jian
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 4
%P 242~248
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0242

T1 - Human bone marrow-derived mesenchymal stem cells transplanted into damaged rabbit heart to improve heart function
A1 - WANG Jian-an
A1 - FAN You-qi
A1 - LI Chang-ling
A1 - HE Hong
A1 - SUN Yong
A1 - LV Bin-jian
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 4
SP - 242
EP - 248
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0242

Objective: The present study was designed to test whether transplantation of human bone marrow-derived mesenchymal stem cells (hMSCs) in New Zealand rabbits with myocardial infarction can improve heart function; and whether engrafted donor cells can survive and transdifferentiated into cardiomyocytes. Methods: Twenty milliliters bone marrow was obtained from healthy men by bone biopsy. A gradient centrifugation method was used to separate bone marrow cells (BMCs) and red blood cells. BMCs were incubated for 48 h and then washed with phosphate-buffered saline (PBS). The culture medium was changed twice a week for 28 d. Finally, hematopoietic cells were washed away to leave only MSCs. Human MSCs (hMSCs) were premarked by BrdU 72 h before the transplantation. Thirty-four New Zealand rabbits were randomly divided into myocardial infarction (MI) control group and cell treated group, which received hMSCs (MI+MSCs) through intramyocardial injection, while the control group received the same volume of PBS. Myocardial infarction was induced by ligation of the left coronary artery. Cell treated rabbits were treated with 5×106 MSCs transplanted into the infarcted region after ligation of the coronary artery for 1 h, and the control group received the same volume of PBS. Cyclosporin A (oral solution; 10 mg/kg) was provided alone, 24 h before surgery and once a day after MI for 4 weeks. Echocardiography was measured in each group before the surgery and 4 weeks after the surgery to test heart function change. The hearts were harvested for HE staining and immunohistochemical studies after MI and cell transplantation for 4 weeks. Results: Our data showed that cardiac function was significantly improved by hMSC transplantation in rabbit infarcted hearts 4 weeks after MI (ejection fraction: 0.695±0.038 in the cell treated group (n=12) versus 0.554±0.065 in the control group (n=13) (P<0.05). Surviving hMSCs were identified by BrdU positive spots in infarcted region and transdifferentiated into cardiomyocytes characterized with a positive cardiac phenotype: troponin I. Conclusion: transplantation of hMSCs could transdifferentiate into cardiomyocytes and regenerate vascular structures, contributing to functional improvement.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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